PMID- 26138884
OWN - NLM
STAT- MEDLINE
DCOM- 20160328
LR  - 20190212
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 36
IP  - 3
DP  - 2015
TI  - Regulation of Na(+)/H(+) Exchanger in Dendritic Cells by Akt1.
PG  - 1237-49
LID - 10.1159/000430293 [doi]
AB  - BACKGROUND/AIMS: Dendritic cells (DCs), antigen-presenting cells critically 
      important for primary immune response and establishment of immunological memory, 
      are activated by bacterial lipopolysaccharides (LPS) resulting in stimulation of 
      Na(+)/H(+) exchanger, ROS formation and migration. The effects are dependent on 
      phosphoinositide 3 (PI3) kinase and paralleled by Akt phosphorylation. The 
      present study explored the contribution of the Akt isoform Akt1. METHODS: 
      Cytosolic pH (pH(i)) (2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein 
      [BCECF] fluorescence), Na(+)/H(+) exchanger activity (Na(+) dependent 
      realkalinization after an ammonium pulse), cell volume (forward scatter in FACS 
      analysis), and ROS production (2',7'-dichlorodihydrofluorescein diacetate [DCFDA] 
      fluorescence) were determined in DCs isolated from bone marrow of mice lacking 
      functional Akt1/PKBalpha (akt1(-/-)) and their wild type littermates (akt1(+/+)). 
      RESULTS: Forward scatter was lower in akt1(-/-) than in akt1(+/+) DCs, whereas 
      pH(i), Na(+)/H(+) exchanger activity and ROS formation were less in untreated 
      akt1(-/-) and akt1(+/+) DCs. Exposure of DCs to LPS was followed by increase of 
      forward scatter and ROS formation to a similar extent in akt1(-/-) and in 
      akt1(+/+) DCs. A 4 hours treatment with either LPS (1microg/ml) or 
      tert-butylhydroperoxide (tBOOH, 5 microM) significantly stimulated Na(+)/H(+) 
      exchanger activity in both genotypes, effects, however, significantly blunted in 
      akt1(-/-) DCs. CONCLUSION: The present observations demonstrate that Akt1 is 
      required for the full stimulation of Na(+)/H(+) exchanger activity by LPS or 
      oxidative stress in dendritic cells.
CI  - (c) 2015 S. Karger AG, Basel.
FAU - Zhou, Yuetao
AU  - Zhou Y
AD  - Department of Physiology, University of Tubingen, Germany.
FAU - Pasham, Venkanna
AU  - Pasham V
FAU - Chatterjee, Soumya
AU  - Chatterjee S
FAU - Rotte, Anand
AU  - Rotte A
FAU - Yang, Wenting
AU  - Yang W
FAU - Bhandaru, Madhuri
AU  - Bhandaru M
FAU - Singh, Yogesh
AU  - Singh Y
FAU - Lang, Florian
AU  - Lang F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150630
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Fluoresceins)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Sodium-Hydrogen Exchangers)
RN  - 85138-49-4 (2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein)
RN  - 955VYL842B (tert-Butylhydroperoxide)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.1 (Akt1 protein, mouse)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology/drug effects/*metabolism
MH  - Cell Movement/drug effects
MH  - Cell Size/drug effects
MH  - Cytosol/drug effects/*metabolism
MH  - Dendritic Cells/cytology/drug effects/*metabolism
MH  - Female
MH  - Fluoresceins
MH  - Fluorescent Dyes
MH  - Gene Expression Regulation
MH  - Hydrogen-Ion Concentration
MH  - Lipopolysaccharides/pharmacology
MH  - Mice
MH  - Mice, Knockout
MH  - Oxidative Stress
MH  - Phosphatidylinositol 3-Kinases/genetics/metabolism
MH  - Phosphorylation/drug effects
MH  - Primary Cell Culture
MH  - Proto-Oncogene Proteins c-akt/deficiency/*genetics
MH  - Reactive Oxygen Species/metabolism
MH  - Signal Transduction
MH  - Sodium-Hydrogen Exchangers/*genetics/metabolism
MH  - tert-Butylhydroperoxide/pharmacology
EDAT- 2015/07/04 06:00
MHDA- 2016/03/29 06:00
CRDT- 2015/07/04 06:00
PHST- 2015/05/05 00:00 [accepted]
PHST- 2015/07/04 06:00 [entrez]
PHST- 2015/07/04 06:00 [pubmed]
PHST- 2016/03/29 06:00 [medline]
AID - 000430293 [pii]
AID - 10.1159/000430293 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2015;36(3):1237-49. doi: 10.1159/000430293. Epub 2015 Jun 
      30.