PMID- 2613923
OWN - NLM
STAT- MEDLINE
DCOM- 19900309
LR  - 20190501
IS  - 0021-9746 (Print)
IS  - 1472-4146 (Electronic)
IS  - 0021-9746 (Linking)
VI  - 42
IP  - 12
DP  - 1989 Dec
TI  - Assessment of immunoglobulin-M immunosorbent agglutination assay (ISAGA) for 
      detecting toxoplasma specific IgM.
PG  - 1291-5
AB  - An immunoglobulin-M immunosorbent agglutination assay (ISAGA) was introduced to 
      detect toxoplasma specific IgM. This assay incorporates mu chain capture and use 
      of entire toxoplasma trophozoites as an antigen source. The performance of the 
      ISAGA was compared with that of a double sandwich enzyme linked immunosorbent 
      assay (DS-ELISA) currently used in the Public Health Laboratory Service 
      Toxoplasma Reference Laboratories. The ISAGA was found to be more sensitive than 
      DS-ELISA but there was no demonstrable difference in the specificity or 
      reproducibility between the two assays. The ISAGA is suitable for the diagnosis 
      of acute toxoplasmosis in immunocompetent patients and as a screening test for 
      recent infection in pregnant women. The persistence of ISAGA reactivity, however, 
      is such that additional serological assessment is required to define the risk of 
      congenital infection.
FAU - Duffy, K T
AU  - Duffy KT
AD  - Public Health Laboratory Service, St George's Hospital, London.
FAU - Wharton, P J
AU  - Wharton PJ
FAU - Johnson, J D
AU  - Johnson JD
FAU - New, L
AU  - New L
FAU - Holliman, R E
AU  - Holliman RE
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Clin Pathol
JT  - Journal of clinical pathology
JID - 0376601
RN  - 0 (Antibodies, Protozoan)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Immunosorbents)
SB  - IM
MH  - *Agglutination Tests
MH  - Animals
MH  - Antibodies, Protozoan/*analysis
MH  - Antibody Specificity
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Immunoglobulin M/*analysis
MH  - Immunosorbents
MH  - Toxoplasma/*immunology
PMC - PMC502063
EDAT- 1989/12/01 00:00
MHDA- 1989/12/01 00:01
PMCR- 1992/12/01
CRDT- 1989/12/01 00:00
PHST- 1989/12/01 00:00 [pubmed]
PHST- 1989/12/01 00:01 [medline]
PHST- 1989/12/01 00:00 [entrez]
PHST- 1992/12/01 00:00 [pmc-release]
AID - 10.1136/jcp.42.12.1291 [doi]
PST - ppublish
SO  - J Clin Pathol. 1989 Dec;42(12):1291-5. doi: 10.1136/jcp.42.12.1291.