PMID- 26142204
OWN - NLM
STAT- MEDLINE
DCOM- 20160503
LR  - 20150804
IS  - 1874-1754 (Electronic)
IS  - 0167-5273 (Linking)
VI  - 197
DP  - 2015 Oct 15
TI  - Role of vascular peroxidase 1 in senescence of endothelial cells in diabetes 
      rats.
PG  - 182-91
LID - S0167-5273(15)30035-8 [pii]
LID - 10.1016/j.ijcard.2015.06.098 [doi]
AB  - BACKGROUND: Reactive oxygen species (ROS) is thought as a major reason of 
      vascular injury in diabetes. Vascular peroxidase 1 (VPO1) is a newly found 
      peroxidase playing an important role in inducing oxidative stress. In the present 
      experiment, we tested the role of VPO1 in senescence of endothelial cells in 
      streptozotocin (STZ)-induced diabetic rats and cultured endothelial cells. 
      METHODS: Blood samples were collected from carotid arteries. Vasodilator 
      responses to acetylcholine (Ach) in the isolated aortic rings were measured, 
      serum concentration of glucose, tumor necrosis factor-alpha (TNF-alpha) and monocyte 
      chemoattractant protein-1 (MCP-1) and the expression of VPO1 in the aorta were 
      determined. Endothelial cells were treated with high glucose or H2O2, the 
      concentrations of MCP-1, TNF-alpha and hypochlorous acid (HOCl) and the expression of 
      VPO1 were determined. shRNA of VPO1 was used for mechanism research in cultured 
      cells. RESULTS: Vasodilator responses to Ach were impaired markedly and the serum 
      concentrations of glucose, TNF-alpha and MCP-1 were significantly increased in 
      diabetic rats. The expression of VPO1 in the aorta was upregulated in diabetic 
      rats. High glucose treatment significantly decreased cell viability and elevated 
      the levels of MCP-1, TNF-alpha and HOCl and upregulated the expression of VPO1. H2O2 
      treatment significantly induced cellular senescence, inhibited eNOS expression 
      and NO production. The effects of high glucose and H2O2 were attenuated by shRNA 
      interference of VPO1. CONCLUSIONS: VPO1 plays an important role in senescence of 
      endothelial cells and endothelial dysfunction by induction of oxidative stress 
      and inflammatory reaction in type 2 diabetic rats.
CI  - Copyright (c) 2015 Elsevier Ireland Ltd. All rights reserved.
FAU - Liu, Si-Yu
AU  - Liu SY
AD  - Department of Pharmacology, School of Pharmaceutical Science, Central South 
      University, Changsha 410078, China; Department of Pharmacology, Xiangnan College, 
      Chenzhou 423000, China.
FAU - Yuan, Qiong
AU  - Yuan Q
AD  - Department of Pharmacology, School of Pharmaceutical Science, Central South 
      University, Changsha 410078, China; Department of Pharmacology, Medical College, 
      Wuhan University of Science and Technology, Wuhan 430081, China.
FAU - Li, Xiao-Hui
AU  - Li XH
AD  - Department of Pharmacology, School of Pharmaceutical Science, Central South 
      University, Changsha 410078, China.
FAU - Hu, Chang-Ping
AU  - Hu CP
AD  - Department of Pharmacology, School of Pharmaceutical Science, Central South 
      University, Changsha 410078, China.
FAU - Hu, Rong
AU  - Hu R
AD  - Department of Pharmacology, School of Pharmaceutical Science, Central South 
      University, Changsha 410078, China.
FAU - Zhang, Guo-Gang
AU  - Zhang GG
AD  - Department of Cardiovascular Medicine, Xiangya Hospital, Central South 
      University, Changsha 410078, China.
FAU - Li, Dai
AU  - Li D
AD  - Department of Pharmacology, School of Pharmaceutical Science, Central South 
      University, Changsha 410078, China.
FAU - Li, Yuan-Jian
AU  - Li YJ
AD  - Department of Pharmacology, School of Pharmaceutical Science, Central South 
      University, Changsha 410078, China. Electronic address: yuan_jianli@yahoo.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150627
PL  - Netherlands
TA  - Int J Cardiol
JT  - International journal of cardiology
JID - 8200291
RN  - 0 (Hemeproteins)
RN  - 0 (RNA, Messenger)
RN  - EC 1.11.1- (vascular peroxidase, rat)
RN  - EC 1.11.1.- (Peroxidases)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Diabetes Mellitus, Experimental/enzymology/*genetics/physiopathology
MH  - Diabetes Mellitus, Type 2/enzymology/genetics/physiopathology
MH  - Endothelium, Vascular/*enzymology/physiopathology
MH  - *Gene Expression Regulation
MH  - Hemeproteins/biosynthesis/*genetics
MH  - Humans
MH  - Male
MH  - *Oxidative Stress
MH  - Peroxidases/biosynthesis/*genetics
MH  - RNA, Messenger/*genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Real-Time Polymerase Chain Reaction
MH  - *Vasodilation
OTO - NOTNLM
OT  - Endothelial cells
OT  - Senescence
OT  - Type 2 diabetes mellitus
OT  - Vascular peroxidase
EDAT- 2015/07/05 06:00
MHDA- 2016/05/04 06:00
CRDT- 2015/07/05 06:00
PHST- 2015/01/25 00:00 [received]
PHST- 2015/06/06 00:00 [revised]
PHST- 2015/06/23 00:00 [accepted]
PHST- 2015/07/05 06:00 [entrez]
PHST- 2015/07/05 06:00 [pubmed]
PHST- 2016/05/04 06:00 [medline]
AID - S0167-5273(15)30035-8 [pii]
AID - 10.1016/j.ijcard.2015.06.098 [doi]
PST - ppublish
SO  - Int J Cardiol. 2015 Oct 15;197:182-91. doi: 10.1016/j.ijcard.2015.06.098. Epub 
      2015 Jun 27.