PMID- 26142428
OWN - NLM
STAT- MEDLINE
DCOM- 20160309
LR  - 20181202
IS  - 1574-6941 (Electronic)
IS  - 0168-6496 (Linking)
VI  - 91
IP  - 8
DP  - 2015 Aug
TI  - In situ identification and quantification of starch-hydrolyzing bacteria attached 
      to barley and corn grain in the rumen of cows fed barley-based diets.
PG  - fiv077
LID - fiv077 [pii]
LID - 10.1093/femsec/fiv077 [doi]
AB  - Cereal grains rich in starch are widely used to meet the energy demands of 
      high-producing beef and dairy cattle. Bacteria are important players in starch 
      digestion in the rumen, and thus play an important role in the hydrolysis and 
      fermentation of cereal grains. However, our understanding of the composition of 
      the rumen starch-hydrolyzing bacteria (SHB) is limited. In this study, BODIPY FL 
      DQ starch staining combined with fluorescence in situ hybridization (FISH) and 
      quantitative FISH were applied to label, identify and quantify SHB possessing 
      active cell-surface-associated (CSA) alpha-amylase activity in the rumen of heifers 
      fed barley-based diets. When individual cells of SHB with active CSA alpha-amylase 
      activity were enumerated, they constituted 19-23% of the total bacterial cells 
      attached to particles of four different cultivars of barley grain and corn. 
      Quantitative FISH revealed that up to 70-80% of these SHB were members of 
      Ruminococcaceae in the phylum Firmicutes but were not Streptococcus bovis, 
      Ruminobacter amylophilus, Succinomonas amylolytica, Bifidobacterium spp. or 
      Butyrivibrio fibrisolvens, all of whose amylolytic activities have been 
      demonstrated previously in vitro. The proportion of barley grain in the diet had 
      a large impact on the percentage abundance of total SHB and Ruminococcaceae SHB 
      in these animals.
CI  - (c) FEMS 2015. All rights reserved. For permissions, please e-mail: 
      journals.permissions@oup.com.
FAU - Xia, Yun
AU  - Xia Y
AD  - Key laboratory of Special Biological Resource Development and Utilization of 
      Universities of Yunnan Province, Kunming University, Kunming 750021, China 
      Lethbridge Research Centre, Agriculture and Agri-Food Canada, 5403 1st Avenue 
      South, Lethbridge, Alberta T1J 4B1, Canada.
FAU - Kong, Yunhong
AU  - Kong Y
AD  - Key laboratory of Special Biological Resource Development and Utilization of 
      Universities of Yunnan Province, Kunming University, Kunming 750021, China.
FAU - Seviour, Robert
AU  - Seviour R
AD  - Department of Microbiology, La Trobe University, Bundoora, VIC 3228, Australia.
FAU - Yang, Hee-Eun
AU  - Yang HE
AD  - Lethbridge Research Centre, Agriculture and Agri-Food Canada, 5403 1st Avenue 
      South, Lethbridge, Alberta T1J 4B1, Canada.
FAU - Forster, Robert
AU  - Forster R
AD  - Lethbridge Research Centre, Agriculture and Agri-Food Canada, 5403 1st Avenue 
      South, Lethbridge, Alberta T1J 4B1, Canada.
FAU - Vasanthan, Thavaratnam
AU  - Vasanthan T
AD  - Department of Food and Nutritional Sciences, University of Alberta, Edmonton, 
      Alberta T6G 2P5, Canada.
FAU - McAllister, Tim
AU  - McAllister T
AD  - Lethbridge Research Centre, Agriculture and Agri-Food Canada, 5403 1st Avenue 
      South, Lethbridge, Alberta T1J 4B1, Canada Tim.McAllister@agr.gc.ca.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150702
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
RN  - 9005-25-8 (Starch)
RN  - EC 3.2.1.1 (alpha-Amylases)
SB  - IM
MH  - *Animal Feed
MH  - Animal Nutritional Physiological Phenomena
MH  - Animals
MH  - Cattle
MH  - Diet/veterinary
MH  - Digestion
MH  - Edible Grain/*metabolism
MH  - Female
MH  - Fermentation
MH  - Hordeum/metabolism
MH  - Hydrolysis
MH  - In Situ Hybridization, Fluorescence
MH  - Rumen/*microbiology
MH  - Ruminococcus/enzymology/*metabolism
MH  - Starch/metabolism
MH  - Zea mays/metabolism
MH  - alpha-Amylases/*metabolism
OTO - NOTNLM
OT  - BODIPY FL DQTM starch staining combined with FISH
OT  - cell-surface-associated amylase activity
OT  - in situ identification
OT  - ruminal starch-hydrolyzing organisms
EDAT- 2015/07/05 06:00
MHDA- 2016/03/10 06:00
CRDT- 2015/07/05 06:00
PHST- 2015/06/29 00:00 [accepted]
PHST- 2015/07/05 06:00 [entrez]
PHST- 2015/07/05 06:00 [pubmed]
PHST- 2016/03/10 06:00 [medline]
AID - fiv077 [pii]
AID - 10.1093/femsec/fiv077 [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2015 Aug;91(8):fiv077. doi: 10.1093/femsec/fiv077. Epub 2015 
      Jul 2.