PMID- 26143318
OWN - NLM
STAT- MEDLINE
DCOM- 20160913
LR  - 20150914
IS  - 1873-3557 (Electronic)
IS  - 1386-1425 (Linking)
VI  - 151
DP  - 2015
TI  - Petal-like CdS nanospheres-based electrochemiluminescence aptasensor for 
      detection of IgE with gold nanoparticles amplification.
PG  - 274-9
LID - S1386-1425(15)30027-5 [pii]
LID - 10.1016/j.saa.2015.06.104 [doi]
AB  - A facile label-free electrochemiluminescence (ECL) aptasensor was designed for 
      sensitive detection of human immunoglobulin E (IgE) using petal-like CdS 
      nanospheres and Au nanoparticles (AuNPs) as sensing platform. To construct the 
      aptasensor, petal-like CdS nanospheres as ECL emitter were firstly synthesized 
      and immobilized on the chitosan-coated glassy carbon electrode (GCE) surface. 
      Chitosan was coated on the CdS/CS/GCE again with two coating numbers to produce a 
      stable ECL signal and facilitate subsequent AuNPs immobilization. The 
      construction of aptasensor was achieved after IgE aptamer was adsorbed onto the 
      AuNPs. The detection of IgE was performed upon the incubation of the interface 
      with target protein IgE. Under the optimum conditions, the ECL signal decreased 
      depending linearly on the logarithmic value of IgE concentration ranging from 
      5.0x10(-13) to 1.0x10(-9)M with a regression equation of I=-15254.8-2129.3 logc 
      (R(2)=0.996). The detection limit was experimentally found to be 8.0x10(-14)M. 
      The applicability of the constructed aptasensor was demonstrated in the 
      determination of IgE in human serum samples.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Cao, Juntao
AU  - Cao J
AD  - College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 
      464000, China; State Key Laboratory of Analytical Chemistry for Life Science, 
      School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, 
      China.
FAU - Wang, Hui
AU  - Wang H
AD  - College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 
      464000, China.
FAU - Liu, Yanming
AU  - Liu Y
AD  - College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 
      464000, China. Electronic address: liuym9518@sina.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150629
PL  - England
TA  - Spectrochim Acta A Mol Biomol Spectrosc
JT  - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
JID - 9602533
RN  - 0 (Cadmium Compounds)
RN  - 0 (Sulfides)
RN  - 057EZR4Z7Q (cadmium sulfide)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Biosensing Techniques/methods
MH  - Cadmium Compounds/*chemistry
MH  - Electrochemical Techniques/methods
MH  - *Electrodes
MH  - Gold/*chemistry
MH  - Humans
MH  - Immunoglobulin E/*blood
MH  - Luminescent Measurements/methods
MH  - Metal Nanoparticles/*chemistry
MH  - Nanospheres/*chemistry
MH  - Sulfides/*chemistry
OTO - NOTNLM
OT  - Electrochemiluminescence
OT  - IgE
OT  - Label-free aptasensor
OT  - Petal-like CdS nanospheres
EDAT- 2015/07/06 06:00
MHDA- 2016/09/14 06:00
CRDT- 2015/07/06 06:00
PHST- 2015/03/01 00:00 [received]
PHST- 2015/06/02 00:00 [revised]
PHST- 2015/06/25 00:00 [accepted]
PHST- 2015/07/06 06:00 [entrez]
PHST- 2015/07/06 06:00 [pubmed]
PHST- 2016/09/14 06:00 [medline]
AID - S1386-1425(15)30027-5 [pii]
AID - 10.1016/j.saa.2015.06.104 [doi]
PST - ppublish
SO  - Spectrochim Acta A Mol Biomol Spectrosc. 2015;151:274-9. doi: 
      10.1016/j.saa.2015.06.104. Epub 2015 Jun 29.