PMID- 26144236
OWN - NLM
STAT- MEDLINE
DCOM- 20160408
LR  - 20240323
IS  - 2053-230X (Electronic)
IS  - 2053-230X (Linking)
VI  - 71
IP  - Pt 7
DP  - 2015 Jul
TI  - Synthesis, purification and crystallographic studies of the C-terminal sterol 
      carrier protein type 2 (SCP-2) domain of human hydroxysteroid dehydrogenase-like 
      protein 2.
PG  - 901-5
LID - 10.1107/S2053230X15008559 [doi]
AB  - Human hydroxysteroid dehydrogenase-like protein 2 (HSDL2) is a member of the 
      short-chain dehydrogenase/reductase (SDR) subfamily of oxidoreductases and 
      contains an N-terminal catalytic domain and a C-termianl sterol carrier protein 
      type 2 (SCP-2) domain. In this study, the C-terminal SCP-2 domain of human HSDL2, 
      including residues Lys318-Arg416, was produced in Escherichia coli, purified and 
      crystallized. X-ray diffraction data were collected to 2.10 A resolution. The 
      crystal belonged to the trigonal space group P3(1)21 (or P3(2)21), with unit-cell 
      parameters a = b = 70.4, c = 60.6 A, alpha = beta = 90, gamma = 120 degrees . Two protein molecules 
      are present in the asymmetric unit, resulting in a Matthews coefficient of 
      2.16 A(3) Da(-1) and an approximate solvent content of 43%.
FAU - Cheng, Zhong
AU  - Cheng Z
AD  - Key Laboratory of Bioactive Substances and Resources Utilization of Chinese 
      Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, 
      Chinese Academy of Medical Sciences and Peking Union Medical College, 151 
      Malianwa North Road, Haidian District, Beijing 100193, People's Republic of 
      China.
FAU - Li, Yao
AU  - Li Y
AD  - Key Laboratory of Bioactive Substances and Resources Utilization of Chinese 
      Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, 
      Chinese Academy of Medical Sciences and Peking Union Medical College, 151 
      Malianwa North Road, Haidian District, Beijing 100193, People's Republic of 
      China.
FAU - Sui, Chun
AU  - Sui C
AD  - Key Laboratory of Bioactive Substances and Resources Utilization of Chinese 
      Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, 
      Chinese Academy of Medical Sciences and Peking Union Medical College, 151 
      Malianwa North Road, Haidian District, Beijing 100193, People's Republic of 
      China.
FAU - Sun, Xiaobo
AU  - Sun X
AD  - Key Laboratory of Bioactive Substances and Resources Utilization of Chinese 
      Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, 
      Chinese Academy of Medical Sciences and Peking Union Medical College, 151 
      Malianwa North Road, Haidian District, Beijing 100193, People's Republic of 
      China.
FAU - Xie, Yong
AU  - Xie Y
AD  - Key Laboratory of Bioactive Substances and Resources Utilization of Chinese 
      Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, 
      Chinese Academy of Medical Sciences and Peking Union Medical College, 151 
      Malianwa North Road, Haidian District, Beijing 100193, People's Republic of 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150627
PL  - United States
TA  - Acta Crystallogr F Struct Biol Commun
JT  - Acta crystallographica. Section F, Structural biology communications
JID - 101620319
RN  - 0 (Carrier Proteins)
RN  - 0 (sterol carrier proteins)
RN  - EC 1.1.- (HSDL2 protein, human)
RN  - EC 1.1.- (Hydroxysteroid Dehydrogenases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Carrier Proteins/*chemical synthesis/genetics/*isolation & purification
MH  - Crystallization
MH  - Crystallography, X-Ray/methods
MH  - Humans
MH  - Hydroxysteroid Dehydrogenases/*chemical synthesis/genetics/*isolation & 
      purification
MH  - Molecular Sequence Data
MH  - Protein Structure, Tertiary
PMC - PMC4498712
OTO - NOTNLM
OT  - SCP-2 domian
OT  - X-ray diffraction
OT  - crystallization
OT  - human HSDL2
EDAT- 2015/07/07 06:00
MHDA- 2016/04/09 06:00
PMCR- 2017/07/01
CRDT- 2015/07/07 06:00
PHST- 2015/01/27 00:00 [received]
PHST- 2015/05/01 00:00 [accepted]
PHST- 2015/07/07 06:00 [entrez]
PHST- 2015/07/07 06:00 [pubmed]
PHST- 2016/04/09 06:00 [medline]
PHST- 2017/07/01 00:00 [pmc-release]
AID - S2053230X15008559 [pii]
AID - rl5097 [pii]
AID - 10.1107/S2053230X15008559 [doi]
PST - ppublish
SO  - Acta Crystallogr F Struct Biol Commun. 2015 Jul;71(Pt 7):901-5. doi: 
      10.1107/S2053230X15008559. Epub 2015 Jun 27.