PMID- 26150947
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150707
LR  - 20201001
IS  - 2215-0161 (Print)
IS  - 2215-0161 (Electronic)
IS  - 2215-0161 (Linking)
VI  - 1
DP  - 2014
TI  - The GCaMP3 - A GFP-based calcium sensor for imaging calcium dynamics in the human 
      malaria parasite Plasmodium falciparum.
PG  - 151-4
LID - 10.1016/j.mex.2014.08.005 [doi]
AB  - Calcium (Ca(2+)) signaling pathways are vital for all eukaryotic cells. It is 
      well established that changes in Ca(2+) concentration can modulate several 
      physiological processes such as muscle contraction, neurotransmitter secretion 
      and metabolic regulation (Giacomello et al. (2007) [1], Rizzuto and Pozzan (2003) 
      [2]). In the complex life cycle of Plasmodium falciparum, the causative agent of 
      human malaria, Ca(2+) is involved in the processes of protein secretion, 
      motility, cell invasion, cell progression and parasite egress from red blood 
      cells (RBCs) (Koyama et al. (2009) [3]). The generation of P. falciparum 
      expressing genetically encoded calcium indicators (GECIs) represents an 
      innovation in the study of calcium signaling. This development will provide new 
      insight on calcium homeostasis and signaling in P. falciparum. In addition, these 
      novel transgenic parasites, PfGCaMP3, is a useful tool for screening and 
      identifying new classes of compounds with anti-malarial activity. This represents 
      a possibility of interfering with signaling pathways controlling parasite growth 
      and development. Our new method differs from previous loading protocols (Garcia 
      et al. (1996) [4]; Beraldo et al. (2007) [5]) since:*It provides a novel method 
      for imaging calcium fluctuations in the cytosol of P. falciparum, without signal 
      interference from the host cell and invasive loading protocols.*This technique 
      could also be expanded for imaging calcium in different subcellular 
      compartments.*It will be helpful in the development of novel antimalarials 
      capable of disrupting calcium homeostasis during the intraerythrocytic cycle of 
      P. falciparum.
FAU - Borges-Pereira, Lucas
AU  - Borges-Pereira L
AD  - Departamento de Fisiologia, Instituto de Biociencias, Universidade de Sao Paulo, 
      Sao Paulo, Brazil ; Departamento de Parasitologia, Instituto de Ciencias 
      Biomedicas, Universidade de Sao Paulo, Brazil.
FAU - Campos, Bruna R K L
AU  - Campos BR
AD  - Departamento de Fisiologia, Instituto de Biociencias, Universidade de Sao Paulo, 
      Sao Paulo, Brazil.
FAU - Garcia, Celia R S
AU  - Garcia CR
AD  - Departamento de Fisiologia, Instituto de Biociencias, Universidade de Sao Paulo, 
      Sao Paulo, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20140827
PL  - Netherlands
TA  - MethodsX
JT  - MethodsX
JID - 101639829
PMC - PMC4472923
OTO - NOTNLM
OT  - Calcium
OT  - Drug screening
OT  - GCaMP3
OT  - GECIs
OT  - GFP
OT  - Malaria
OT  - Plasmodium falciparum
EDAT- 2014/01/01 00:00
MHDA- 2014/01/01 00:01
PMCR- 2014/08/27
CRDT- 2015/07/08 06:00
PHST- 2014/08/06 00:00 [received]
PHST- 2014/08/08 00:00 [accepted]
PHST- 2015/07/08 06:00 [entrez]
PHST- 2014/01/01 00:00 [pubmed]
PHST- 2014/01/01 00:01 [medline]
PHST- 2014/08/27 00:00 [pmc-release]
AID - S2215-0161(14)20066-5 [pii]
AID - 10.1016/j.mex.2014.08.005 [doi]
PST - epublish
SO  - MethodsX. 2014 Aug 27;1:151-4. doi: 10.1016/j.mex.2014.08.005. eCollection 2014.