PMID- 26151606
OWN - NLM
STAT- MEDLINE
DCOM- 20160817
LR  - 20151110
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 210
DP  - 2015 Dec 2
TI  - Endogenization of mouse mammary tumor virus (MMTV)-like elements in genomes of 
      pikas (Ochotona sp.).
PG  - 22-6
LID - S0168-1702(15)30003-4 [pii]
LID - 10.1016/j.virusres.2015.06.021 [doi]
AB  - Despite the finding in European rabbit and other leporid genomes of the first 
      ever described endogenous lentivirus and of a European rabbit exclusive 
      endogenous gammaretrovirus, until now no exogenous retroviruses have been 
      isolated in Lagomorpha species. Nevertheless, looking for the presence of 
      endogenous retroviruses (ERVs) in the species genomes could lead to the discovery 
      of retroviral lineages yet to be found in Lagomorpha. Different mammalian genomes 
      harbor endogenous viral sequences phylogenetically close to the betaretrovirus 
      mouse mammary tumor virus (MMTV), propelling us to look for such retroviral 
      "fossil" in American pika (Ochotona princeps) and European rabbit (Oryctolagus 
      cuniculus) genomes. By performing genomic mining using MMTV gag and LTR as query 
      sequences, we found that such viral elements were absent from the European rabbit 
      genome. Oppositely, significant matches were found in American pika, and more 
      importantly, a nearly complete MMTV-like virus (Pika-BERV) was identified. Using 
      Pika-BERV gag and LTR as templates, we found similar sequences endogenized in 
      different pika (Ochotona sp.) species. The orthology of the LTR flanking region 
      between some pika species supported shared ancestry of specific endogenous 
      betaretroviruses, while in other pika species similar sequences, but not 
      orthologous, should have resulted from independent insertions. Our study supports 
      the possible existence of infecting exogenous betaretroviruses for a long term, 
      after the divergence of Ochotonidae from Leporidae, but yet to be identified.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Lemos de Matos, Ana
AU  - Lemos de Matos A
AD  - CIBIO - Centro de Investigacao em Biodiversidade e Recursos Geneticos/InBIO 
      Laboratorio Associado, Universidade do Porto, Campus Agrario de Vairao, Rua Padre 
      Armando Quintas, 4485-661 Vairao, Portugal; Departamento de Biologia, Faculdade 
      de Ciencias, Universidade do Porto, 4169-007 Porto, Portugal. Electronic address: 
      anamatos@ufl.edu.
FAU - de Sousa-Pereira, Patricia
AU  - de Sousa-Pereira P
AD  - CIBIO - Centro de Investigacao em Biodiversidade e Recursos Geneticos/InBIO 
      Laboratorio Associado, Universidade do Porto, Campus Agrario de Vairao, Rua Padre 
      Armando Quintas, 4485-661 Vairao, Portugal; Departamento de Biologia, Faculdade 
      de Ciencias, Universidade do Porto, 4169-007 Porto, Portugal.
FAU - Lissovsky, Andrey A
AU  - Lissovsky AA
AD  - Zoological Museum, Moscow State Lomonosov University, 125009, B. Nikitskaya 6, 
      Moscow, Russia.
FAU - van der Loo, Wessel
AU  - van der Loo W
AD  - CIBIO - Centro de Investigacao em Biodiversidade e Recursos Geneticos/InBIO 
      Laboratorio Associado, Universidade do Porto, Campus Agrario de Vairao, Rua Padre 
      Armando Quintas, 4485-661 Vairao, Portugal; Departamento de Biologia, Faculdade 
      de Ciencias, Universidade do Porto, 4169-007 Porto, Portugal.
FAU - Melo-Ferreira, Jose
AU  - Melo-Ferreira J
AD  - CIBIO - Centro de Investigacao em Biodiversidade e Recursos Geneticos/InBIO 
      Laboratorio Associado, Universidade do Porto, Campus Agrario de Vairao, Rua Padre 
      Armando Quintas, 4485-661 Vairao, Portugal.
FAU - Cui, Jie
AU  - Cui J
AD  - Program in Emerging Infectious Diseases, Duke-NUS Graduate Medical School, 
      Singapore 169857, Singapore.
FAU - Esteves, Pedro J
AU  - Esteves PJ
AD  - CIBIO - Centro de Investigacao em Biodiversidade e Recursos Geneticos/InBIO 
      Laboratorio Associado, Universidade do Porto, Campus Agrario de Vairao, Rua Padre 
      Armando Quintas, 4485-661 Vairao, Portugal; Departamento de Biologia, Faculdade 
      de Ciencias, Universidade do Porto, 4169-007 Porto, Portugal; CITS - Centro de 
      Investigacao em Tecnologias da Saude, IPSN, CESPU, 4585-116 Gandra, Portugal. 
      Electronic address: pjesteves@cibio.up.pt.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150704
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
SB  - IM
MH  - Animals
MH  - Endogenous Retroviruses/classification/*genetics/*isolation & purification
MH  - Lagomorpha/*virology
MH  - Mammary Tumor Virus, Mouse/classification/*genetics
MH  - Rabbits/virology
OTO - NOTNLM
OT  - Endogenous betaretrovirus (EnBV)
OT  - Endogenous retrovirus (ERV)
OT  - Mouse mammary tumor virus (MMTV)
OT  - genomic mining
OT  - genus Ochotona
EDAT- 2015/07/08 06:00
MHDA- 2016/08/18 06:00
CRDT- 2015/07/08 06:00
PHST- 2015/05/09 00:00 [received]
PHST- 2015/06/24 00:00 [revised]
PHST- 2015/06/25 00:00 [accepted]
PHST- 2015/07/08 06:00 [entrez]
PHST- 2015/07/08 06:00 [pubmed]
PHST- 2016/08/18 06:00 [medline]
AID - S0168-1702(15)30003-4 [pii]
AID - 10.1016/j.virusres.2015.06.021 [doi]
PST - ppublish
SO  - Virus Res. 2015 Dec 2;210:22-6. doi: 10.1016/j.virusres.2015.06.021. Epub 2015 
      Jul 4.