PMID- 26152641
OWN - NLM
STAT- MEDLINE
DCOM- 20160719
LR  - 20150815
IS  - 1099-1492 (Electronic)
IS  - 0952-3480 (Linking)
VI  - 28
IP  - 9
DP  - 2015 Sep
TI  - Multi-echo susceptibility-weighted imaging and histology of open-field 
      blast-induced traumatic brain injury in a rat model.
PG  - 1069-77
LID - 10.1002/nbm.3351 [doi]
AB  - Blast-induced traumatic brain injury is on the rise, predominantly as a result of 
      the use of improvised explosive devices, resulting in undesirable 
      neuropsychological dysfunctions, as demonstrated in both animals and humans. This 
      study investigated the effect of open-field blast injury on the rat brain using 
      multi-echo, susceptibility-weighted imaging (SWI). Multi-echo SWI provided phase 
      maps with better signal-to-noise ratio (SNR) and contrast-to-noise ratio (CNR), 
      making it a sensitive technique for brain injury. Male Sprague-Dawley rats were 
      subjected to a survivable blast of 180 kPa. The visibility of blood vessels of 
      varying sizes improved with multi-echo SWI. Reduced signal intensity from major 
      vessels post-blast indicates increased deoxyhaemoglobin. Relative cerebral blood 
      flow was computed from filtered phase SWI images using inferred changes in oxygen 
      saturation from major blood vessels. Cerebral blood flow decreased significantly 
      at day 3 and day 5 post-blast compared with that pre-blast. This was 
      substantiated by the upregulation of beta-amyloid precursor protein (beta-APP), a 
      marker of ischaemia, in the neuronal perikaya of the cerebral cortex, as observed 
      by immunofluorescence, and in the cortical tissue by western blot analysis. Our 
      findings indicate the presence of brain ischaemia in post-blast acute phase of 
      injury with possible recovery subsequently. Our results from cerebrovascular 
      imaging, histology and staining provide an insight into the ischaemic state of 
      the brain post-blast and may be useful for prognosis and outcome.
CI  - Copyright (c) 2015 John Wiley & Sons, Ltd.
FAU - Verma, Sanjay Kumar
AU  - Verma SK
AD  - Laboratory of Molecular Imaging, Singapore Bioimaging Consortium, Singapore.
FAU - Kan, Enci Mary
AU  - Kan EM
AD  - Defence Medical and Environmental Research Institute, DSO National Laboratories, 
      Singapore.
AD  - Department of Anatomy, Yong Loo Lin School of Medicine, National University of 
      Singapore, Singapore.
FAU - Lu, Jia
AU  - Lu J
AD  - Defence Medical and Environmental Research Institute, DSO National Laboratories, 
      Singapore.
AD  - Department of Anatomy, Yong Loo Lin School of Medicine, National University of 
      Singapore, Singapore.
FAU - Ng, Kian Chye
AU  - Ng KC
AD  - Defence Medical and Environmental Research Institute, DSO National Laboratories, 
      Singapore.
FAU - Ling, Eng Ang
AU  - Ling EA
AD  - Department of Anatomy, Yong Loo Lin School of Medicine, National University of 
      Singapore, Singapore.
FAU - Seramani, Sankar
AU  - Seramani S
AD  - Laboratory of Molecular Imaging, Singapore Bioimaging Consortium, Singapore.
FAU - Kn, Bhanu Prakash
AU  - Kn BP
AD  - Laboratory of Molecular Imaging, Singapore Bioimaging Consortium, Singapore.
FAU - Wong, Yong Chiat
AU  - Wong YC
AD  - Defence Medical and Environmental Research Institute, DSO National Laboratories, 
      Singapore.
FAU - Tan, Mui Hong
AU  - Tan MH
AD  - Defence Medical and Environmental Research Institute, DSO National Laboratories, 
      Singapore.
FAU - Velan, S Sendhil
AU  - Velan SS
AD  - Laboratory of Molecular Imaging, Singapore Bioimaging Consortium, Singapore.
AD  - Clinical Imaging Research Centre, Agency for Science, Technology and Research, 
      Singapore.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150707
PL  - England
TA  - NMR Biomed
JT  - NMR in biomedicine
JID - 8915233
RN  - 0 (Amyloid beta-Protein Precursor)
SB  - IM
MH  - Amyloid beta-Protein Precursor/analysis
MH  - Animals
MH  - Blast Injuries/*pathology
MH  - Brain Injuries/*pathology
MH  - Cerebrovascular Circulation
MH  - Disease Models, Animal
MH  - Magnetic Resonance Imaging/*methods
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal-To-Noise Ratio
OTO - NOTNLM
OT  - blast injury
OT  - cerebral blood flow
OT  - immunohistochemistry
OT  - susceptibility-weighted imaging
OT  - traumatic brain injury
EDAT- 2015/07/15 06:00
MHDA- 2016/07/20 06:00
CRDT- 2015/07/09 06:00
PHST- 2014/07/09 00:00 [received]
PHST- 2015/05/18 00:00 [revised]
PHST- 2015/06/03 00:00 [accepted]
PHST- 2015/07/09 06:00 [entrez]
PHST- 2015/07/15 06:00 [pubmed]
PHST- 2016/07/20 06:00 [medline]
AID - 10.1002/nbm.3351 [doi]
PST - ppublish
SO  - NMR Biomed. 2015 Sep;28(9):1069-77. doi: 10.1002/nbm.3351. Epub 2015 Jul 7.