PMID- 26152792
OWN - NLM
STAT- MEDLINE
DCOM- 20151026
LR  - 20150812
IS  - 1600-0463 (Electronic)
IS  - 0903-4641 (Linking)
VI  - 123
IP  - 9
DP  - 2015 Sep
TI  - Identification of Toxoplasma gondii protein fractions induce immune response 
      against melanoma in mice.
PG  - 800-9
LID - 10.1111/apm.12420 [doi]
AB  - Dendritic cells (DCs) play a crucial role in the initiation of adaptive immune 
      responses against tumor cells. We recently found that protein components of 
      Toxoplasma gondii (T. gondii) could mature DCs efficiently. Therefore, in this 
      study, we aimed to find the most effective protein components of T. gondii which 
      are able to mature DCs and consequently instruct immune responses in 
      tumor-bearing mice. Soluble tachyzoite antigens (STAgs) were fractionated by 
      ammonium sulfate precipitation and subsequently by anion-exchange HPLC. Immature 
      DCs (iDCs) were treated by these protein fractions and were monitored for 
      IL-12p70 and IL-10 production. Moreover, the capacity of mature DCs (mDCs) to 
      induce lymphocyte proliferation was investigated. Ultimately, we analyzed the 
      ability of mDCs in instructing immune responses in tumor-bearing mice. We found 
      that ammonium sulfate fraction one (A1) matured-DCs produced higher IL-12 level 
      and IL-12/IL-10 ratio; therefore, this fraction was selected for further 
      fractionation by anion-exchange HPLC. The results showed that anion-exchange HPLC 
      fraction 14 (C14) matured-DCs secrete higher levels of IL-12p70 and 
      IL-12p70/IL-10 ratio. Survival of the mice matured by A1 fraction increased 
      significantly compared to other groups. Moreover, SDS-PAGE electrophoresis showed 
      that different obtained fractions have distinct proteins based on their size. 
      These results demonstrate that two protein fractions of T. gondii are able to 
      mature DCs more efficient.
CI  - (c) 2015 APMIS. Published by John Wiley & Sons Ltd.
FAU - Boghozian, Roobina
AU  - Boghozian R
AD  - Department of Immunology, School of Medicine, Tehran University of Medical 
      Sciences, Tehran, Iran.
FAU - Saei, Azad
AU  - Saei A
AD  - Department of Immunology, School of Medicine, Tehran University of Medical 
      Sciences, Tehran, Iran.
FAU - Mirzaei, Reza
AU  - Mirzaei R
AD  - Department of Immunology, School of Medicine, Tehran University of Medical 
      Sciences, Tehran, Iran.
FAU - Jamali, Arezoo
AU  - Jamali A
AD  - Department of Laboratory Sciences, School of Paramedicine, Tehran University of 
      Medical Sciences, Tehran, Iran.
FAU - Vaziri, Behrooz
AU  - Vaziri B
AD  - Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
FAU - Razavi, Alireza
AU  - Razavi A
AD  - Department of Immunology, School of Public Health, Tehran University of Medical 
      Sciences, Tehran, Iran.
FAU - Hadjati, Jamshid
AU  - Hadjati J
AD  - Department of Immunology, School of Medicine, Tehran University of Medical 
      Sciences, Tehran, Iran.
LA  - eng
PT  - Journal Article
DEP - 20150708
PL  - Denmark
TA  - APMIS
JT  - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
JID - 8803400
RN  - 0 (Protozoan Proteins)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Cell Proliferation/physiology
MH  - Dendritic Cells/immunology
MH  - Female
MH  - Interleukin-10/immunology
MH  - Interleukin-12/immunology
MH  - Lymphocyte Activation/immunology
MH  - Melanoma/*immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Protozoan Proteins/*immunology
MH  - Toxoplasma/*immunology
OTO - NOTNLM
OT  - Dendritic cells
OT  - Toxoplasma gondii
OT  - immune response
OT  - tumor
EDAT- 2015/07/15 06:00
MHDA- 2015/10/27 06:00
CRDT- 2015/07/09 06:00
PHST- 2014/09/30 00:00 [received]
PHST- 2015/06/04 00:00 [accepted]
PHST- 2015/07/09 06:00 [entrez]
PHST- 2015/07/15 06:00 [pubmed]
PHST- 2015/10/27 06:00 [medline]
AID - 10.1111/apm.12420 [doi]
PST - ppublish
SO  - APMIS. 2015 Sep;123(9):800-9. doi: 10.1111/apm.12420. Epub 2015 Jul 8.