PMID- 26155179
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150709
LR  - 20200930
IS  - 1426-3912 (Print)
IS  - 1644-4124 (Electronic)
IS  - 1426-3912 (Linking)
VI  - 40
IP  - 1
DP  - 2015
TI  - Comparison between magnetic activated cell sorted monocytes and monocyte 
      adherence techniques for in vitro generation of immature dendritic cells: an 
      Egyptian trial.
PG  - 18-24
LID - 10.5114/ceji.2015.50828 [doi]
AB  - INTRODUCTION: Dendritic cells (DCs) are the most efficient antigen presenting 
      cells, which are considered a central component of the immune system for their 
      extraordinary capacity to initiate and modulate the immune responses elicited 
      upon recognition of infectious agents. This has made them a major focus of 
      interest in the conception of immunotherapeutic vaccine strategies. AIM OF THE 
      STUDY: To standardise a protocol for in vitro differentiation of human peripheral 
      blood monocytes into immature DCs (iDCs) upon treatment with specific growth 
      factors and to compare two monocyte isolation methods including magnetic 
      activated cell sorted (MACS) monocytes by CD14(+) immuno-magnetic beads and 
      monocytes separated by adherence. MATERIAL AND METHODS: Immature DCs were 
      generated from monocytes of human peripheral blood in the presence of 
      granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-4 
      after in vitro culture for seven days. Cultured cells were stained with surface 
      markers of iDCs: FITC-anti-CD14, PE-anti-CD11c, PE-anti-CD1a, PE-Cy5-anti-HLA-DR, 
      and PE-anti-CD83 for flow cytometry analysis. RESULTS: We found that the 
      viability of MACS-DCs was higher than DCs derived from monocytes separated by 
      adherence (median 50 and interquartile range 45-50 vs. 25 and 10-30, 
      respectively; p < 0.001). Flow cytometry analysis revealed that the median 
      interquartile percentages of MACS-DCs expressing CD14(-) was significantly higher 
      compared to the DCs derived from monocytes separated by adherence (median 80.2 
      and interquartile range 77.7-80.7 vs. 40.2 and 30.4-40.6, respectively; p < 
      0.001). However, MACS-DCs expressed the same levels of CD11c, CD1a, and HLA-DR as 
      well as CD83 compared to the DCs derived from monocytes separated by adherence 
      with p value > 0.05. CONCLUSIONS: Both positively selected monocytes and 
      monocytes separated by adherence procedure gave the same results as regards cell 
      surface marker expression, although the DCs purity and viability using MACS 
      separated monocytes were better.
FAU - El-Sahrigy, Sally Ahmed
AU  - El-Sahrigy SA
AD  - Pediatrics Medical Research Division, National Research Centre, Egypt.
FAU - Mohamed, Nesrine Aly
AU  - Mohamed NA
AD  - Clinical Pathology and Immunology Faculty of Medicine, Ain Shams University, 
      Egypt.
FAU - Talkhan, Hala Ahmed
AU  - Talkhan HA
AD  - Clinical Pathology and Immunology Faculty of Medicine, Ain Shams University, 
      Egypt.
FAU - Rahman, Azza M Abdel
AU  - Rahman AM
AD  - Pediatrics Medical Research Division, National Research Centre, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20150422
PL  - Poland
TA  - Cent Eur J Immunol
JT  - Central-European journal of immunology
JID - 9702239
PMC - PMC4472535
OTO - NOTNLM
OT  - adherence
OT  - cell separation
OT  - dendritic cell
OT  - phenotype
EDAT- 2015/07/15 06:00
MHDA- 2015/07/15 06:01
PMCR- 2015/01/01
CRDT- 2015/07/09 06:00
PHST- 2014/12/22 00:00 [received]
PHST- 2015/02/04 00:00 [accepted]
PHST- 2015/07/09 06:00 [entrez]
PHST- 2015/07/15 06:00 [pubmed]
PHST- 2015/07/15 06:01 [medline]
PHST- 2015/01/01 00:00 [pmc-release]
AID - 50828 [pii]
AID - 10.5114/ceji.2015.50828 [doi]
PST - ppublish
SO  - Cent Eur J Immunol. 2015;40(1):18-24. doi: 10.5114/ceji.2015.50828. Epub 2015 Apr 
      22.