PMID- 26155181
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150709
LR  - 20220408
IS  - 1426-3912 (Print)
IS  - 1644-4124 (Electronic)
IS  - 1426-3912 (Linking)
VI  - 40
IP  - 1
DP  - 2015
TI  - Proteomic and immunological identification of two new allergens from silkworm 
      (Bombyx mori L.) pupae.
PG  - 30-4
LID - 10.5114/ceji.2015.50830 [doi]
AB  - This study explored food allergy caused by eating silkworm (Bombyx mori L.) 
      pupae, a traditionally accepted food and animal feed in East and Southeast Asia, 
      and identified two new allergens by proteomic and immunological methods. Proteins 
      isolated from silkworm pupae were separated by two-dimensional gel 
      electrophoresis (2-DE); pooled sera from patients allergic to silkworm pupa 
      proteins were used to detect immunoglobulin E (IgE)-binding proteins by western 
      blotting, and allergens specific for silkworm pupa consumption-caused allergy 
      were visualised with the ECL reagents. The selected allergen proteins were 
      further identified by matrix-assisted laser desorption ionisation time-of-flight 
      mass spectrometry (MALDI-TOF-MS) analysis. Finally, chitinase and paramyosin were 
      identified as silkworm pupa proteins showing strong immunoglobulin (IgE)-binding 
      reaction. Analysis of the sequence homology of the two proteins using the 
      AllergenOnline database indicated that chitinase and paramyosin shared 24.8% and 
      62.8% sequence homology with known allergens Der f 18 (Dermatophagoides farinae) 
      and Der p 11 (Dermatophagoides pteronyssinus), respectively. Our results shed 
      light on the understanding and treatment of silkworm pupa allergy.
FAU - Zhao, Xiangjie
AU  - Zhao X
AD  - College of Light Industry and Food Sciences, South China University of 
      Technology, Guangdong, P.R. of China ; Sericultural & Agri-Food Research 
      Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, P.R. of China ; 
      School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, 
      Jiangsu, P.R. of China.
FAU - Li, Lin
AU  - Li L
AD  - College of Light Industry and Food Sciences, South China University of 
      Technology, Guangdong, P.R. of China.
FAU - Kuang, Zheshi
AU  - Kuang Z
AD  - Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural 
      Sciences, Guangzhou, P.R. of China.
FAU - Luo, Guoqing
AU  - Luo G
AD  - Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural 
      Sciences, Guangzhou, P.R. of China.
FAU - Li, Bing
AU  - Li B
AD  - College of Light Industry and Food Sciences, South China University of 
      Technology, Guangdong, P.R. of China.
LA  - eng
PT  - Journal Article
DEP - 20150422
PL  - Poland
TA  - Cent Eur J Immunol
JT  - Central-European journal of immunology
JID - 9702239
PMC - PMC4472537
OTO - NOTNLM
OT  - MALDI-TOF-MS
OT  - allergen
OT  - chitinase
OT  - paramyosin
OT  - silkworm (Bombyx mori L.) pupa
OT  - western blot
EDAT- 2015/07/15 06:00
MHDA- 2015/07/15 06:01
PMCR- 2015/01/01
CRDT- 2015/07/09 06:00
PHST- 2014/10/29 00:00 [received]
PHST- 2015/02/10 00:00 [accepted]
PHST- 2015/07/09 06:00 [entrez]
PHST- 2015/07/15 06:00 [pubmed]
PHST- 2015/07/15 06:01 [medline]
PHST- 2015/01/01 00:00 [pmc-release]
AID - 50830 [pii]
AID - 10.5114/ceji.2015.50830 [doi]
PST - ppublish
SO  - Cent Eur J Immunol. 2015;40(1):30-4. doi: 10.5114/ceji.2015.50830. Epub 2015 Apr 
      22.