PMID- 26155202
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150709
LR  - 20201001
IS  - 1738-6055 (Print)
IS  - 2233-7660 (Electronic)
IS  - 1738-6055 (Linking)
VI  - 31
IP  - 2
DP  - 2015 Jun
TI  - Tumor necrosis factor-alpha deficiency impairs host defense against Streptococcus 
      pneumoniae.
PG  - 78-85
LID - 10.5625/lar.2015.31.2.78 [doi]
AB  - Streptococcus pneumoniae is a major human pathogen that is involved in 
      community-acquired pneumonia. Tumor necrosis factor-alpha (TNF-alpha) is a 
      pro-inflammatory cytokine that activates immune responses against infection, 
      invasion, injury, or inflammation. To study the role of TNF-alpha during S. 
      pneumoniae infection, a murine pneumococcal pneumonia model was used. We 
      intranasally infected C57BL/6J wild-type (WT) and TNF-alpha knockout (KO) mice with 
      S. pneumoniae D39 serotype 2. In TNF-alpha KO mice, continuous and distinct loss of 
      body weight, and low survival rates were observed. Bacterial counts in the lungs 
      and blood of TNF-alpha KO mice were significantly higher than those in WT mice. 
      Histopathological lesions in the spleen of TNF-alpha KO mice were more severe than 
      those in WT mice. In TNF-alpha KO mice, severe depletion of white pulp was observed 
      and the number of apoptotic cells was significantly increased. Interferon-gamma 
      (IFN-gamma), IL-12p70 and IL-10 levels in serum were significantly increased in TNF-alpha 
      KO mice. TNF-alpha is clearly involved in the regulation of S. pneumoniae infections. 
      Early death and low survival rates of TNF-alpha KO mice were likely caused by a 
      combination of impaired bacterial clearance and damage to the spleen. Our 
      findings suggest that TNF-alpha plays a critical role in protecting the host from 
      systemic S. pneumoniae infection.
FAU - Jeong, Dong-Gu
AU  - Jeong DG
AD  - Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk 
      University, Seoul, Korea.
FAU - Seo, Jin-Hee
AU  - Seo JH
AD  - Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk 
      University, Seoul, Korea. ; Laboratory Animal Facility, Korea Institute of 
      Radiological and Medical Sciences, Seoul, Korea.
FAU - Heo, Seung-Ho
AU  - Heo SH
AD  - Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk 
      University, Seoul, Korea. ; Asan Institute for Life Sciences, University of Ulsan 
      College of Medicine, Seoul, Korea.
FAU - Choi, Yang-Kyu
AU  - Choi YK
AD  - Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk 
      University, Seoul, Korea.
FAU - Jeong, Eui-Suk
AU  - Jeong ES
AD  - Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk 
      University, Seoul, Korea. ; Laboratory Animal Center, Daegu-Gyeongbuk Medical 
      Innovation Foundation, Daegu, Korea.
LA  - eng
PT  - Journal Article
DEP - 20150626
PL  - England
TA  - Lab Anim Res
JT  - Laboratory animal research
JID - 101560684
PMC - PMC4490149
OTO - NOTNLM
OT  - Streptococcus pneumoniae
OT  - Tumor necrosis factor-alpha knockout
OT  - pneumonia
COIS- Conflict of interests: The authors declare that there is no financial conflict of 
      interests to publish these results.
EDAT- 2015/07/15 06:00
MHDA- 2015/07/15 06:01
PMCR- 2015/06/01
CRDT- 2015/07/09 06:00
PHST- 2015/04/23 00:00 [received]
PHST- 2015/05/21 00:00 [revised]
PHST- 2015/05/31 00:00 [accepted]
PHST- 2015/07/09 06:00 [entrez]
PHST- 2015/07/15 06:00 [pubmed]
PHST- 2015/07/15 06:01 [medline]
PHST- 2015/06/01 00:00 [pmc-release]
AID - 10.5625/lar.2015.31.2.78 [doi]
PST - ppublish
SO  - Lab Anim Res. 2015 Jun;31(2):78-85. doi: 10.5625/lar.2015.31.2.78. Epub 2015 Jun 
      26.