PMID- 26155677
OWN - NLM
STAT- MEDLINE
DCOM- 20150910
LR  - 20240109
IS  - 0019-5189 (Print)
IS  - 0019-5189 (Linking)
VI  - 53
IP  - 6
DP  - 2015 Jun
TI  - Phlorotannins from Brown Algae: inhibition of advanced glycation end products 
      formation in high glucose induced Caenorhabditis elegans.
PG  - 371-9
AB  - Advanced Glycation End products (AGE) generated in a non enzymatic protein 
      glycation process are frequently associated with diabetes, aging and other 
      chronic diseases. Here, we explored the protective effect of phlorotannins from 
      brown algae Padina pavonica, Sargassum polycystum and Turbinaria ornata against 
      AGEs formation. Phlorotannins were extracted from brown algae with methanol and 
      its purity was analyzed by TLC and RP-HPLC-DAD. Twenty five grams of P. pavonica, 
      S. polycystum, T. ornata yielded 27.6 +/- 0.8 mug/ml, 37.7 mug/ml and 37.1 +/- 0.74 
      mug/ml of phloroglucinol equivalent of phlorotannins, respectively. Antioxidant 
      potentials were examined through DPPH assay and their IC50 values were P. 
      pavonica (30.12 +/- 0.99 mug), S. polycystum (40.9 +/- 1.2 mug) and T. ornata (22.9 +/- 
      1.3 mug), which was comparatively lesser than the control ascorbic acid (46 +/- 0.2 
      mug). Further, anti-AGE activity was examined in vitro by BSA-glucose assay with 
      the extracted phlorotannins of brown algae (P. pavonica, 15.16 +/- 0.26 mug/ml; S. 
      polycystum, 35.245 +/- 2.3 mug/ml; T. ornata, 22.7 +/- 0.3 mug/ml), which revealed the 
      required concentration to inhibit 50% of albumin glycation (IC50) were lower for 
      extracts than controls (phloroglucinol, 222.33 +/- 4.9 mug/ml; thiamine, 263 mug/ml). 
      Furthermore, brown algal extracts containing phlorotannins (100 mul) exhibited 
      protective effects against AGE formation in vivo in C. elegans with induced 
      hyperglycemia.
FAU - Shakambari, Ganeshan
AU  - Shakambari G
FAU - Ashokkumar, Balasubramaniem
AU  - Ashokkumar B
FAU - Varalakshmi, Perumal
AU  - Varalakshmi P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - India
TA  - Indian J Exp Biol
JT  - Indian journal of experimental biology
JID - 0233411
RN  - 0 (Antioxidants)
RN  - 0 (Glycation End Products, Advanced)
RN  - 0 (Tannins)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Antioxidants/chemistry/*pharmacology
MH  - Caenorhabditis elegans/*drug effects/metabolism
MH  - Glucose/*metabolism
MH  - Glycation End Products, Advanced/analysis/*antagonists & inhibitors/metabolism
MH  - Phaeophyceae/*chemistry
MH  - Tannins/chemistry/*pharmacology
EDAT- 2015/07/15 06:00
MHDA- 2015/09/12 06:00
CRDT- 2015/07/10 06:00
PHST- 2015/07/10 06:00 [entrez]
PHST- 2015/07/15 06:00 [pubmed]
PHST- 2015/09/12 06:00 [medline]
PST - ppublish
SO  - Indian J Exp Biol. 2015 Jun;53(6):371-9.