PMID- 2615757 OWN - NLM STAT- MEDLINE DCOM- 19900315 LR - 20240109 IS - 0026-8925 (Print) IS - 0026-8925 (Linking) VI - 219 IP - 1-2 DP - 1989 Oct TI - The gene family encoding the Arabidopsis thaliana translation elongation factor EF-1 alpha: molecular cloning, characterization and expression. PG - 106-12 AB - The gene family encoding the Arabidopsis thaliana translation elongation factor (EF-1 alpha) was analysed. This family contains four genes (A1-A4) organized in a similar manner in different varieties of Arabidopsis. Based upon both their physical separation and a comparison of their sequences, it is suggested that the A4 gene and the A1, A2, and A3 genes constitute two distinct subfamilies within the genome. By introducing chimaeric gene constructs into Arabidopsis cells, we showed that the A1 gene promoter mediates a transient expression about twofold higher than that obtained using the CaMV 35 S promoter. This expression depends on a 348 bp DNA fragment extending from -982 to -634 bp upstream of the initiation codon. This element contains a characteristic telomeric sequence (AACCCTAA) which is also found in the promoters of the A2 and A4 genes as well as in the promoters of the Drosophila EF-1 alpha F1 gene and of several highly expressed plant genes. FAU - Axelos, M AU - Axelos M AD - Laboratoire des Relations Plantes-Microorganismes, CNRS-INRA, Castanet-Tolosan, France. FAU - Bardet, C AU - Bardet C FAU - Liboz, T AU - Liboz T FAU - Le Van Thai, A AU - Le Van Thai A FAU - Curie, C AU - Curie C FAU - Lescure, B AU - Lescure B LA - eng SI - GENBANK/X16430 PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Germany TA - Mol Gen Genet JT - Molecular & general genetics : MGG JID - 0125036 RN - 0 (Peptide Elongation Factor 1) RN - 0 (Peptide Elongation Factors) SB - IM MH - Amino Acid Sequence MH - Base Sequence MH - Chimera MH - Cloning, Molecular MH - Exons MH - *Genes, Plant MH - Introns MH - Molecular Sequence Data MH - *Multigene Family MH - Peptide Elongation Factor 1 MH - Peptide Elongation Factors/*genetics MH - Plants/*genetics MH - Promoter Regions, Genetic MH - Protoplasts/metabolism MH - Restriction Mapping MH - Sequence Homology, Nucleic Acid MH - Transcription, Genetic EDAT- 1989/10/01 00:00 MHDA- 1989/10/01 00:01 CRDT- 1989/10/01 00:00 PHST- 1989/10/01 00:00 [pubmed] PHST- 1989/10/01 00:01 [medline] PHST- 1989/10/01 00:00 [entrez] AID - 10.1007/BF00261164 [doi] PST - ppublish SO - Mol Gen Genet. 1989 Oct;219(1-2):106-12. doi: 10.1007/BF00261164.