PMID- 26158004
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150709
LR  - 20201001
IS  - 2329-423X (Print)
IS  - 2329-4248 (Electronic)
IS  - 2329-423X (Linking)
VI  - 2
IP  - 2
DP  - 2015 Apr
TI  - Comparison of genetically encoded calcium indicators for monitoring action 
      potentials in mammalian brain by two-photon excitation fluorescence microscopy.
PG  - 021014
LID - 10.1117/1.NPh.2.2.021014 [doi]
LID - 021014
AB  - Imaging calcium transients associated with neuronal activity has yielded 
      important insights into neural physiology. Genetically encoded calcium indicators 
      (GECIs) offer conspicuous potential advantages for this purpose, including 
      exquisite targeting. While the catalogue of available GECIs is steadily growing, 
      many newly developed sensors that appear promising in vitro or in model cells 
      appear to be less useful when expressed in mammalian neurons. We have, therefore, 
      evaluated the performance of GECIs from two of the most promising families of 
      sensors, G-CaMPs [Nat. Biotechnol.2(2), 137-141 (2001)] and GECOs [Science2(2), 
      1888-1891 (2011)], for monitoring action potentials in rat brain. Specifically, 
      we used two-photon excitation fluorescence microscopy to compare calcium 
      transients detected by G-CaMP3; GCaMP6f; G-CaMP7; Green-GECO1.0, 1.1 and 1.2; 
      Blue-GECO; Red-GECO; Rex-GECO0.9; Rex-GECO1; Carmine-GECO; Orange-GECO; and 
      Yellow-GECO1s. After optimizing excitation wavelengths, we monitored fluorescence 
      signals associated with increasing numbers of action potentials evoked by current 
      injection in CA1 pyramidal neurons in rat organotypic hippocampal slices. Some 
      GECIs, particularly Green-GECO1.2, GCaMP6f, and G-CaMP7, were able to detect 
      single action potentials with high reliability. By virtue of greatest sensitivity 
      and fast kinetics, G-CaMP7 may be the best currently available GECI for 
      monitoring calcium transients in mammalian neurons.
FAU - Podor, Borbala
AU  - Podor B
AD  - Dalhousie University , Department of Physiology and Biophysics, Halifax, Nova 
      Scotia B3H 4R2, Canada.
FAU - Hu, Yi-Ling
AU  - Hu YL
AD  - Dalhousie University , Department of Physiology and Biophysics, Halifax, Nova 
      Scotia B3H 4R2, Canada.
FAU - Ohkura, Masamichi
AU  - Ohkura M
AD  - Saitama University , Brain Science Institute, Saitama 338-8570, Japan.
FAU - Nakai, Junichi
AU  - Nakai J
AD  - Saitama University , Brain Science Institute, Saitama 338-8570, Japan.
FAU - Croll, Roger
AU  - Croll R
AD  - Dalhousie University , Department of Physiology and Biophysics, Halifax, Nova 
      Scotia B3H 4R2, Canada.
FAU - Fine, Alan
AU  - Fine A
AD  - Dalhousie University , Department of Physiology and Biophysics, Halifax, Nova 
      Scotia B3H 4R2, Canada.
LA  - eng
PT  - Journal Article
DEP - 20150430
PL  - United States
TA  - Neurophotonics
JT  - Neurophotonics
JID - 101632875
PMC - PMC4478877
OTO - NOTNLM
OT  - G-CaMP
OT  - GECO
OT  - calcium transients
OT  - genetically encoded calcium indicators
OT  - organotypic hippocampal slice
OT  - two-photon microscopy
EDAT- 2015/07/15 06:00
MHDA- 2015/07/15 06:01
PMCR- 2016/04/30
CRDT- 2015/07/10 06:00
PHST- 2014/12/01 00:00 [received]
PHST- 2015/03/31 00:00 [accepted]
PHST- 2015/07/10 06:00 [entrez]
PHST- 2015/07/15 06:00 [pubmed]
PHST- 2015/07/15 06:01 [medline]
PHST- 2016/04/30 00:00 [pmc-release]
AID - 14080SSR [pii]
AID - 10.1117/1.NPh.2.2.021014 [doi]
PST - ppublish
SO  - Neurophotonics. 2015 Apr;2(2):021014. doi: 10.1117/1.NPh.2.2.021014. Epub 2015 
      Apr 30.