PMID- 26159026
OWN - NLM
STAT- MEDLINE
DCOM- 20160722
LR  - 20181202
IS  - 1003-5370 (Print)
IS  - 1003-5370 (Linking)
VI  - 35
IP  - 5
DP  - 2015 May
TI  - [Study on the Mechanism of Three Kinds Extracts of Qingxin Kaiqiao Recipe in 
      Improving Learning and Memory Capabilities of AD Rats].
PG  - 595-602
AB  - OBJECTIVE: To explore the mechanism of three kinds extracts (saponins, volatile 
      components, polysaccharide components) of Qingxin Kaiqiao Recipe (QKR) in 
      improving learning and memory capabilities of Alzheimer's disease (AD) rats. 
      METHODS: A controlled comparison method was used. Totally 56 male SD rats were 
      randomly divided into seven groups, i.e., the normal control group, the 
      sham-operation group, the model group, the Aricept group, the saponin group, the 
      benzene group, and the polysaccharide group, 8 in each group. AD rat model was 
      established by bilateral hippocampus injection of Abeta1-40 (2 microL, 2.5 microg/microL). The 
      next day after modeling rats in the saponin group, the benzene group, and the 
      polysaccharide group, the saponin group, the Aricept group were intragastrically 
      administered with saponin (at the daily dose of 9 mL/kg, 2.1 g/mL) , benzene (at 
      the daily dose of 3.33 mL/kg, 5.7 g/mL) , polysaccharide (at the daily dose of 
      8.33 mL/kg, 2.28 g/mL), Aricept (at the daily dose of 1.67 mg/kg), respectively, 
      once a day for 2 consecutive weeks from 10 am every day. Equal volume of normal 
      saline was intragastrically administered to rats in the normal control group and 
      the model group. Learning and memory capabilities were detected using water maze 
      2 weeks later. Expression levels of synaptotagmin-1 (Syt-1), interleukin-1beta 
      (IL-1beta), glia fibrillary acidic protein (GFAP), and beta-amyloid precursor protein 
      (betaAPP) in the cortex and hippocampus of AD rats were detected using 
      immunohistochemistry. RESULTS: Learning and memory capabilities could be improved 
      by three kinds extracts of QKR. There was no statistical difference in the escape 
      latency between the polysaccharide group and the model group (P >0. 05). The 
      escape lacency was shortened in the rest treatment groups (P < 0.05). The escape 
      latency was obviously prolonged in three kinds extracts of QKR groups, when 
      compared with the Aricept group (P < 0.05, P < 0.01). Compared with the model 
      group, times for crossing platforms were significantly increased in the saponin 
      group and the Aricept group (P < 0.05). Compared with the Aricept group, average 
      times for crossing platforms were significantly lessened in three kinds extracts 
      of QKR groups (P < 0.01). Compared with the sham-operation group, expression 
      levels of Syt-1, IL-1beta, GFAP, and betaAPP in the cortex and hippocampus were 
      increased in the model group (P < 0.01). Compared with the model group, the 
      expression of cortical Syt-1 increased in the saponin group and the benzene 
      group; the expression of cortical IL-1beta increased in the benzene group and the 
      polysaccharide group; the expression of hippocampal GFAP increased in the three 
      kinds extracts of QKR groups; expression levels of Syt-1, IL-1beta, GFAP, and beta-APP 
      in the cortex and hippocampus decreased in the rest treatment groups (all P < 
      0.05, P < 0.01). Compared with the Aricept group, expression levels of Syt-1, 
      IL-1beta, GFAP, and betaAPP in the cortex and hippocampus were significantly increased 
      in three kinds extracts of QKR groups (P < 0.05, P < 0.01). CONCLUSION: Three 
      kinds extracts of QKR might play roles in anti-AD possibly by decreasing 
      expression levels of Syt-1, IL-1beta, GFAP, and betaAPP in the cortex and hippocampus.
FAU - Hu, Hai-yan
AU  - Hu HY
FAU - Chen, Zhi-yu
AU  - Chen ZY
FAU - Xu, Dong-mei
AU  - Xu DM
FAU - Zhang, Yi-hui
AU  - Zhang YH
FAU - Wang, Yi-ru
AU  - Wang YR
FAU - Wang, Wen-hua
AU  - Wang WH
FAU - Zhang, Xiao-yan
AU  - Zhang XY
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhong Xi Yi Jie He Za Zhi
JT  - Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese 
      journal of integrated traditional and Western medicine
JID - 9211576
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Saponins)
SB  - IM
MH  - *Alzheimer Disease
MH  - Amyloid beta-Protein Precursor
MH  - Animals
MH  - *Disease Models, Animal
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Glial Fibrillary Acidic Protein
MH  - Hippocampus
MH  - Interleukin-1beta
MH  - Learning/*drug effects
MH  - Male
MH  - Memory
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Saponins
EDAT- 2015/07/15 06:00
MHDA- 2016/07/23 06:00
CRDT- 2015/07/11 06:00
PHST- 2015/07/11 06:00 [entrez]
PHST- 2015/07/15 06:00 [pubmed]
PHST- 2016/07/23 06:00 [medline]
PST - ppublish
SO  - Zhongguo Zhong Xi Yi Jie He Za Zhi. 2015 May;35(5):595-602.