PMID- 26161038
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1445-5781 (Print)
IS  - 1447-0578 (Electronic)
IS  - 1445-5781 (Linking)
VI  - 14
IP  - 3
DP  - 2015
TI  - Tumor necrosis factor alpha inhibits ovulation and induces granulosa cell death 
      in rat ovaries.
PG  - 107-115
AB  - PURPOSE: We evaluated the role of tumor necrosis factor alpha (TNFalpha) in rat 
      ovulation and granulosa cell death of ovarian follicles during the periovulatory 
      stage. METHODS: Immature rats primed with pregnant mare serum gonadotropin were 
      injected intraperitoneally with human chorionic gonadotropin (hCG), and TNFalpha was 
      injected into the bursa 48 h later. The total number of released oocytes was 
      counted. Apoptosis was measured with terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) and the expression of cleaved 
      caspase 3 and Bax/Bcl-2. Autophagy was assessed by the expression of light chain 
      protein 3 (LC3) and autophagosomes under transmission electron microscopy. 
      RESULTS: TNFalpha significantly decreased the number of released oocytes, and many 
      unruptured follicles were observed. TUNEL analysis revealed a larger number of 
      apoptotic cells, and the cleaved caspase 3 and Bax/Bcl-2 increased more than that 
      of the control 12 h after hCG administration. Furthermore, the expression of LC3 
      wwas significantly higher than that of the control, and autophagosomes were 
      observed in the cytoplasm. CONCLUSIONS: Our data indicated that TNFalpha is an 
      important mediator of ovulation in terms of decreasing the number of released 
      oocytes and inducing granulosa cell death of unruptured follicles via apoptosis 
      and autophagy for remodeling ovarian tissues.
FAU - Yamamoto, Yuri
AU  - Yamamoto Y
AD  - Department of Obstetrics and Gynecology, The University of Tokushima, Institute 
      for Health Biosciences, 3-18-15 Kuramoto-cho, Tokushima, 770-8503 Japan.
FAU - Kuwahara, Akira
AU  - Kuwahara A
AD  - Department of Obstetrics and Gynecology, The University of Tokushima, Institute 
      for Health Biosciences, 3-18-15 Kuramoto-cho, Tokushima, 770-8503 Japan.
FAU - Taniguchi, Yuka
AU  - Taniguchi Y
AD  - Department of Obstetrics and Gynecology, The University of Tokushima, Institute 
      for Health Biosciences, 3-18-15 Kuramoto-cho, Tokushima, 770-8503 Japan.
FAU - Yamasaki, Mikio
AU  - Yamasaki M
AD  - Department of Obstetrics and Gynecology, The University of Tokushima, Institute 
      for Health Biosciences, 3-18-15 Kuramoto-cho, Tokushima, 770-8503 Japan.
FAU - Tanaka, Yu
AU  - Tanaka Y
AD  - Department of Obstetrics and Gynecology, The University of Tokushima, Institute 
      for Health Biosciences, 3-18-15 Kuramoto-cho, Tokushima, 770-8503 Japan.
FAU - Mukai, Yukari
AU  - Mukai Y
AD  - Department of Obstetrics and Gynecology, The University of Tokushima, Institute 
      for Health Biosciences, 3-18-15 Kuramoto-cho, Tokushima, 770-8503 Japan.
FAU - Yamashita, Mizuho
AU  - Yamashita M
AD  - Department of Obstetrics and Gynecology, The University of Tokushima, Institute 
      for Health Biosciences, 3-18-15 Kuramoto-cho, Tokushima, 770-8503 Japan.
FAU - Matsuzaki, Toshiya
AU  - Matsuzaki T
AD  - Department of Obstetrics and Gynecology, The University of Tokushima, Institute 
      for Health Biosciences, 3-18-15 Kuramoto-cho, Tokushima, 770-8503 Japan.
FAU - Yasui, Toshiyuki
AU  - Yasui T
AD  - Department of Obstetrics and Gynecology, The University of Tokushima, Institute 
      for Health Biosciences, 3-18-15 Kuramoto-cho, Tokushima, 770-8503 Japan.
FAU - Irahara, Minoru
AU  - Irahara M
AD  - Department of Obstetrics and Gynecology, The University of Tokushima, Institute 
      for Health Biosciences, 3-18-15 Kuramoto-cho, Tokushima, 770-8503 Japan.
LA  - eng
PT  - Journal Article
DEP - 20141213
PL  - Japan
TA  - Reprod Med Biol
JT  - Reproductive medicine and biology
JID - 101213278
PMC - PMC4490172
OTO - NOTNLM
OT  - Apoptosis
OT  - Autophagy
OT  - Granulosa cell death
OT  - Ovulation
OT  - TNF-alpha
COIS- Yuri Yamamoto, Akira Kuwahara, Yuka Taniguchi, Mikio Yamasaki, Yu Tanaka, Toshiya 
      Matsuzaki, Toshiyuki Yasui, Minoru Irahara declare that they have no competing 
      interests.
EDAT- 2015/07/15 06:00
MHDA- 2015/07/15 06:01
PMCR- 2014/12/13
CRDT- 2015/07/11 06:00
PHST- 2014/06/27 00:00 [received]
PHST- 2014/11/26 00:00 [accepted]
PHST- 2015/07/11 06:00 [entrez]
PHST- 2015/07/15 06:00 [pubmed]
PHST- 2015/07/15 06:01 [medline]
PHST- 2014/12/13 00:00 [pmc-release]
AID - 201 [pii]
AID - RMB20201 [pii]
AID - 10.1007/s12522-014-0201-5 [doi]
PST - ppublish
SO  - Reprod Med Biol. 2015;14(3):107-115. doi: 10.1007/s12522-014-0201-5. Epub 2014 
      Dec 13.