PMID- 26164455
OWN - NLM
STAT- MEDLINE
DCOM- 20160420
LR  - 20150713
IS  - 1473-558X (Electronic)
IS  - 0959-4965 (Linking)
VI  - 26
IP  - 12
DP  - 2015 Aug 19
TI  - Auditory stimulation modulates CXCL12/CXCR4 expression in postnatal development 
      of the newborn rat cochlea.
PG  - 681-7
LID - 10.1097/WNR.0000000000000408 [doi]
AB  - Sensorineural hearing loss is one of the most common sensory deficits. Recently, 
      inner-ear stem cell therapy has been proposed for auditory afferent 
      rehabilitation. CXCR4 is the primary physiologic receptor for CXC chemokine 
      ligand 12 (CXCL12) and the CXCL12-CXCR4 pathway has been implicated in the 
      process of migration, differentiation, and maturation of vertebrate neural stem 
      cells. In this study, we examined changes in the auditory brainstem response and 
      CXCL12/CXCR4 expression in newborn rat cochleae under different acoustic 
      environments by quantitative real-time PCR, western blot, enzyme-linked 
      immunosorbent assay, immunohistochemistry, and immunofluorescence analyses. Rats 
      were divided randomly into three groups: the augmented acoustic environment (AAE) 
      group, the auditory deprivation (AD) group, and the control group. Auditory 
      brainstem response thresholds were markedly increased in the AAE group and in the 
      AD group. Compared with postnatal day 1, the expression of CXCL12/CXCR4 mRNA and 
      protein under normal acoustic conditions was increased on postnatal day 14 and 
      then decreased on postnatal day 28 in the cochlea. However, on postnatal day 28, 
      CXCL12/CXCR4 expression, as well as its spatiotemporal distribution as detected 
      by immunohistochemistry and immunofluorescence assays, was augmented by AAE 
      treatment and inhibited by AD treatment. Therefore, our results confirmed that 
      auditory stimulation influenced the spatiotemporal expression of CXCL12/CXCR4 in 
      newborn rat cochlea, which might help to unravel the role of the CXCL12-CXCR4 
      pathway in the synaptic contacts and hearing function establishment in rat 
      cochlea development.
CI  - Copyright (c) 2015 Wolters Kluwer Health, Inc. All rights reserved.
FAU - Zhang, Wen
AU  - Zhang W
AD  - aDepartment of Surgery, The Second Affiliated Hospital, Xi'an Jiaotong University 
      bDepartment of Otolaryngology, The Fourth People's Hospital of Shaanxi Province 
      cDepartment of Otolaryngology, Xijing Hospital, Fourth Military Medical 
      University dDepartment of Otolaryngology, Shaanxi Provincial People's Hospital, 
      Xi'an, Shaanxi, People's Republic of China.
FAU - Zhang, Fangjun
AU  - Zhang F
FAU - Han, Yu
AU  - Han Y
FAU - Liu, Hui
AU  - Liu H
FAU - Wang, Ye
AU  - Wang Y
FAU - Yue, Bo
AU  - Yue B
FAU - Chen, Jun
AU  - Chen J
FAU - Chen, Yang
AU  - Chen Y
FAU - Gao, Ya
AU  - Gao Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 0 (CXCL12 protein, rat)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Cxcr4 protein, rat)
RN  - 0 (Receptors, CXCR4)
SB  - IM
MH  - Acoustic Stimulation/*methods
MH  - Animals
MH  - Animals, Newborn
MH  - Chemokine CXCL12/*biosynthesis/genetics
MH  - Cochlea/*growth & development/*metabolism
MH  - *Gene Expression Regulation, Developmental
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, CXCR4/*biosynthesis/genetics
EDAT- 2015/07/15 06:00
MHDA- 2016/04/21 06:00
CRDT- 2015/07/13 06:00
PHST- 2015/07/13 06:00 [entrez]
PHST- 2015/07/15 06:00 [pubmed]
PHST- 2016/04/21 06:00 [medline]
AID - 00001756-201508020-00004 [pii]
AID - 10.1097/WNR.0000000000000408 [doi]
PST - ppublish
SO  - Neuroreport. 2015 Aug 19;26(12):681-7. doi: 10.1097/WNR.0000000000000408.