PMID- 26166570
OWN - NLM
STAT- MEDLINE
DCOM- 20160512
LR  - 20150723
IS  - 2211-1247 (Electronic)
VI  - 12
IP  - 3
DP  - 2015 Jul 21
TI  - A Single Templating RNA in Yeast Telomerase.
PG  - 441-8
LID - S2211-1247(15)00657-9 [pii]
LID - 10.1016/j.celrep.2015.06.045 [doi]
AB  - The number of essential telomerase components in the active ribonucleoprotein 
      (RNP) has important implications for its mechanism of action yet is by and large 
      unknown. We report that two differentially tagged TLC1 RNAs endogenously 
      expressed in a heterozygous diploid and simultaneously detected via multi-color 
      fluorescence in situ hybridization (FISH) experiments do not co-localize. 
      Probabilistic calculations combined with direct quantification of FISH signals 
      demonstrate that the TLC1 RNA indeed occurs as a single molecule in these RNPs. 
      In addition, two differentially tagged reverse-transcriptase subunits could not 
      be co-immunoprecipitated. These results therefore show that, in yeast cells, 
      telomerase is assembled and matured and occurs as a monomer when not on 
      telomeres. Finally, combining these findings with previous evidence leads us to 
      propose that the enzyme also acts as a monomer when elongating telomeres.
CI  - Copyright (c) 2015 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Bajon, Emmanuel
AU  - Bajon E
AD  - Department of Microbiology and Infectious Diseases, Faculty of Medicine, 
      Universite de Sherbrooke, 3201, rue Jean Mignault, Sherbrooke, QC J1E 4K8, 
      Canada.
FAU - Laterreur, Nancy
AU  - Laterreur N
AD  - Department of Microbiology and Infectious Diseases, Faculty of Medicine, 
      Universite de Sherbrooke, 3201, rue Jean Mignault, Sherbrooke, QC J1E 4K8, 
      Canada.
FAU - Wellinger, Raymund J
AU  - Wellinger RJ
AD  - Department of Microbiology and Infectious Diseases, Faculty of Medicine, 
      Universite de Sherbrooke, 3201, rue Jean Mignault, Sherbrooke, QC J1E 4K8, 
      Canada. Electronic address: raymund.wellinger@usherbrooke.ca.
LA  - eng
GR  - MOP97874/Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150709
PL  - United States
TA  - Cell Rep
JT  - Cell reports
JID - 101573691
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - RNA/*genetics/metabolism
MH  - Saccharomyces cerevisiae/genetics/*metabolism
MH  - Saccharomyces cerevisiae Proteins/genetics/*metabolism
MH  - Telomerase/genetics/*metabolism
EDAT- 2015/07/15 06:00
MHDA- 2016/05/14 06:00
CRDT- 2015/07/14 06:00
PHST- 2015/04/15 00:00 [received]
PHST- 2015/06/04 00:00 [revised]
PHST- 2015/06/13 00:00 [accepted]
PHST- 2015/07/14 06:00 [entrez]
PHST- 2015/07/15 06:00 [pubmed]
PHST- 2016/05/14 06:00 [medline]
AID - S2211-1247(15)00657-9 [pii]
AID - 10.1016/j.celrep.2015.06.045 [doi]
PST - ppublish
SO  - Cell Rep. 2015 Jul 21;12(3):441-8. doi: 10.1016/j.celrep.2015.06.045. Epub 2015 
      Jul 9.