PMID- 26167184
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150713
LR  - 20201001
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2015
DP  - 2015
TI  - Role of Bone Marrow-Derived Stem Cells in Polyps Development in Mice with 
      Apc(Min/+) Mutation.
PG  - 354193
LID - 10.1155/2015/354193 [doi]
LID - 354193
AB  - We explored the hypothesis that an altered microenvironment (intestinal 
      adenomatous polyp) could modify the differentiation program of bone 
      marrow-derived stem cells (BMSCs), involving them in colon carcinogenesis. 
      Sublethally irradiated 8-week-old female Apc(Min/+) mice were transplanted with 
      bone marrow (BM) cells obtained from either male age-matched Apc(Min/+) 
      (Apc-Tx-Apc) or wild type (WT) (WT-Tx-Apc) mice. At 4 and 7 weeks after 
      transplantation, BM-derived colonocytes were recognized by colocalization of 
      Y-chromosome and Cdx2 protein (specific colonocyte marker). Polyp number, volume, 
      and grade of dysplasia were not influenced by irradiation/transplantation 
      procedures since they were similar in both untreated female Apc(Min/+) and 
      Apc-Tx-Apc mice. At 4 and 7 weeks after transplantation, a progressive 
      significant reduction of polyp number and volume was observed in WT-Tx-Apc mice. 
      Moreover, the number of WT-Tx-Apc mice with a high-grade dysplastic polyps 
      significantly decreased as compared to Apc-Tx-Apc mice. Finally, at 4 and 7 weeks 
      after transplantation, WT-Tx-Apc mice showed a progressive significant increase 
      of Y+/Cdx2+ cells in "normal" mucosa, whereas, in the adenomatous tissue, 
      Y+/Cdx2+ cells remained substantially unvaried. Our findings demonstrate that WT 
      BMSCs do not participate in polyp development but rather inhibit their growth. 
      The substitution of genotypically altered colonocytes with Y+/Cdx2+ cells 
      probably contributes to this process.
FAU - Barone, Michele
AU  - Barone M
AD  - Gastroenterology Unit, Department of Emergency and Organ Transplantation, 
      University of Bari, Piazza G. Cesare 11, 70124 Bari, Italy.
FAU - Scavo, Maria Principia
AU  - Scavo MP
AD  - Gastroenterology Unit, Department of Emergency and Organ Transplantation, 
      University of Bari, Piazza G. Cesare 11, 70124 Bari, Italy ; Methodist Research 
      Institute, 6670 Bertner Avenue, Houston, TX 77030, USA.
FAU - Licinio, Raffaele
AU  - Licinio R
AD  - Gastroenterology Unit, Department of Emergency and Organ Transplantation, 
      University of Bari, Piazza G. Cesare 11, 70124 Bari, Italy.
FAU - Piombino, Michele
AU  - Piombino M
AD  - Radiotherapy Unit, Diagnostic Imaging Department, Polyclinic Hospital, Piazza G. 
      Cesare 11, 70124 Bari, Italy.
FAU - De Tullio, Nicola
AU  - De Tullio N
AD  - Gastroenterology Unit, Department of Emergency and Organ Transplantation, 
      University of Bari, Piazza G. Cesare 11, 70124 Bari, Italy.
FAU - Mallamaci, Rosanna
AU  - Mallamaci R
AD  - Department of Bioscience, Biotechnology and Biopharmaceutics, University of Bari, 
      Via E. Orabona 4, 70124 Bari, Italy.
FAU - Di Leo, Alfredo
AU  - Di Leo A
AD  - Gastroenterology Unit, Department of Emergency and Organ Transplantation, 
      University of Bari, Piazza G. Cesare 11, 70124 Bari, Italy.
LA  - eng
PT  - Journal Article
DEP - 20150616
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC4488009
EDAT- 2015/07/15 06:00
MHDA- 2015/07/15 06:01
PMCR- 2015/06/16
CRDT- 2015/07/14 06:00
PHST- 2015/03/29 00:00 [received]
PHST- 2015/05/30 00:00 [accepted]
PHST- 2015/07/14 06:00 [entrez]
PHST- 2015/07/15 06:00 [pubmed]
PHST- 2015/07/15 06:01 [medline]
PHST- 2015/06/16 00:00 [pmc-release]
AID - 10.1155/2015/354193 [doi]
PST - ppublish
SO  - Stem Cells Int. 2015;2015:354193. doi: 10.1155/2015/354193. Epub 2015 Jun 16.