PMID- 26168260 OWN - NLM STAT- MEDLINE DCOM- 20160425 LR - 20181113 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 10 IP - 7 DP - 2015 TI - The Complete Genome of a New Betabaculovirus from Clostera anastomosis. PG - e0132792 LID - 10.1371/journal.pone.0132792 [doi] LID - e0132792 AB - Clostera anastomosis (Lepidoptera: Notodontidae) is a defoliating forest insect pest. Clostera anastomosis granulovirus-B (ClasGV-B) belonging to the genus Betabaculovirus of family Baculoviridae has been used for biological control of the pest. Here we reported the full genome sequence of ClasGV-B and compared it to other previously sequenced baculoviruses. The circular double-stranded DNA genome is 107,439 bp in length, with a G+C content of 37.8% and contains 123 open reading frames (ORFs) representing 93% of the genome. ClasGV-B contains 37 baculovirus core genes, 25 lepidopteran baculovirus specific genes, 19 betabaculovirus specific genes, 39 other genes with homologues to baculoviruses and 3 ORFs unique to ClasGV-B. Hrs appear to be absent from the ClasGV-B genome, however, two non-hr repeats were found. Phylogenetic tree based on 37 core genes from 73 baculovirus genomes placed ClasGV-B in the clade b of betabaculoviruses and was most closely related to Erinnyis ello GV (ErelGV). The gene arrangement of ClasGV-B also shared the strongest collinearity with ErelGV but differed from Clostera anachoreta GV (ClanGV), Clostera anastomosis GV-A (ClasGV-A, previously also called CaLGV) and Epinotia aporema GV (EpapGV) with a 20 kb inversion. ClasGV-B genome contains three copies of polyhedron envelope protein gene (pep) and phylogenetic tree divides the PEPs of betabaculoviruses into three major clades: PEP-1, PEP-2 and PEP/P10. ClasGV-B also contains three homologues of P10 which all harbor an N-terminal coiled-coil domain and a C-terminal basic sequence. ClasGV-B encodes three fibroblast growth factor (FGF) homologues which are conserved in all sequenced betabaculoviruses. Phylogenetic analysis placed these three FGFs into different groups and suggested that the FGFs were evolved at the early stage of the betabaculovirus expansion. ClasGV-B is different from previously reported ClasGV-A and ClanGV isolated from Notodontidae in sequence and gene arrangement, indicating the virus is a new notodontid betabaculovirus. FAU - Yin, Feifei AU - Yin F AD - State Key Laboratory of Virology and China Center for Virus Culture Collection, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, PR China; School of Tropical and Laboratory Medicine, Hainan Medical University, Haikou, 571101, PR China. FAU - Zhu, Zheng AU - Zhu Z AD - State Key Laboratory of Virology and China Center for Virus Culture Collection, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, PR China. FAU - Liu, Xiaoping AU - Liu X AD - State Key Laboratory of Virology and China Center for Virus Culture Collection, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, PR China. FAU - Hou, Dianhai AU - Hou D AD - State Key Laboratory of Virology and China Center for Virus Culture Collection, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, PR China. FAU - Wang, Jun AU - Wang J AD - State Key Laboratory of Virology and China Center for Virus Culture Collection, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, PR China. FAU - Zhang, Lei AU - Zhang L AD - State Key Laboratory of Virology and China Center for Virus Culture Collection, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, PR China. FAU - Wang, Manli AU - Wang M AD - State Key Laboratory of Virology and China Center for Virus Culture Collection, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, PR China. FAU - Kou, Zheng AU - Kou Z AD - State Key Laboratory of Virology and China Center for Virus Culture Collection, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, PR China. FAU - Wang, Hualin AU - Wang H AD - State Key Laboratory of Virology and China Center for Virus Culture Collection, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, PR China. FAU - Deng, Fei AU - Deng F AD - State Key Laboratory of Virology and China Center for Virus Culture Collection, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, PR China. FAU - Hu, Zhihong AU - Hu Z AD - State Key Laboratory of Virology and China Center for Virus Culture Collection, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, PR China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150713 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - Amino Acid Sequence MH - Animals MH - Baculoviridae/*genetics/pathogenicity MH - Base Sequence MH - *Genome, Viral MH - Lepidoptera/*virology MH - Molecular Sequence Data MH - Open Reading Frames MH - Phylogeny MH - Sequence Homology, Amino Acid MH - Sequence Homology, Nucleic Acid PMC - PMC4500397 COIS- Competing Interests: The authors have declared that no competing interests exist. EDAT- 2015/07/15 06:00 MHDA- 2016/04/26 06:00 PMCR- 2015/07/13 CRDT- 2015/07/14 06:00 PHST- 2015/05/12 00:00 [received] PHST- 2015/06/19 00:00 [accepted] PHST- 2015/07/14 06:00 [entrez] PHST- 2015/07/15 06:00 [pubmed] PHST- 2016/04/26 06:00 [medline] PHST- 2015/07/13 00:00 [pmc-release] AID - PONE-D-15-20521 [pii] AID - 10.1371/journal.pone.0132792 [doi] PST - epublish SO - PLoS One. 2015 Jul 13;10(7):e0132792. doi: 10.1371/journal.pone.0132792. eCollection 2015.