PMID- 26170030
OWN - NLM
STAT- MEDLINE
DCOM- 20160428
LR  - 20210202
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Linking)
VI  - 126
IP  - 10
DP  - 2015 Sep 3
TI  - Inflammation rapidly reorganizes mouse bone marrow B cells and their environment 
      in conjunction with early IgM responses.
PG  - 1184-92
LID - 10.1182/blood-2015-03-635805 [doi]
AB  - Systemic inflammation perturbs the bone marrow environment by evicting resident B 
      cells and favoring granulopoiesis over lymphopoiesis. Despite these conditions, a 
      subset of marrow B cell remains to become activated and produce potent acute 
      immunoglobulin M (IgM) responses. This discrepancy is currently unresolved and a 
      complete characterization of early perturbations in the B-cell niche has not been 
      undertaken. Here, we show that within a few hours of challenging mice with 
      adjuvant or cecal puncture, B cells accumulate in the bone marrow redistributed 
      away from sinusoid vessels. This response correlates with enhanced sensitivity to 
      CXC chemokine ligand 12 (CXCL12) but not CXCL13 or CC chemokine ligand 21. 
      Concurrently, a number of B-cell survival and differentiation factors are 
      elevated to produce a transiently supportive milieu. Disrupting homing dynamics 
      with a CXC chemokine receptor 4 inhibitor reduced the formation of IgM-secreting 
      cells. These data highlight the rapidity with which peripheral inflammation 
      modifies the marrow compartment, and demonstrate that such modifications regulate 
      acute IgM production within this organ. Furthermore, our study indicates that 
      conversion to a state of emergency granulopoiesis is temporally delayed, allowing 
      B cells opportunity to respond to antigen.
CI  - (c) 2015 by The American Society of Hematology.
FAU - Moreau, Joshua M
AU  - Moreau JM
AD  - Department of Immunology, University of Toronto, Toronto, ON, Canada; Ontario 
      Cancer Institute, Princess Margaret Cancer Centre, University Health Network, 
      Toronto, ON, Canada;
FAU - Berger, Alexandra
AU  - Berger A
AD  - Ontario Cancer Institute, Princess Margaret Cancer Centre, University Health 
      Network, Toronto, ON, Canada;
FAU - Nelles, Megan E
AU  - Nelles ME
AD  - Ontario Cancer Institute, Princess Margaret Cancer Centre, University Health 
      Network, Toronto, ON, Canada;
FAU - Mielnik, Michael
AU  - Mielnik M
AD  - Ontario Cancer Institute, Princess Margaret Cancer Centre, University Health 
      Network, Toronto, ON, Canada; Department of Medical Biophysics and.
FAU - Furlonger, Caren
AU  - Furlonger C
AD  - Ontario Cancer Institute, Princess Margaret Cancer Centre, University Health 
      Network, Toronto, ON, Canada;
FAU - Cen, Selena Y
AU  - Cen SY
AD  - Department of Immunology, University of Toronto, Toronto, ON, Canada; Ontario 
      Cancer Institute, Princess Margaret Cancer Centre, University Health Network, 
      Toronto, ON, Canada;
FAU - Besla, Rickvinder
AU  - Besla R
AD  - Department of Laboratory Medicine and Pathobiology, University of Toronto, 
      Toronto, ON, Canada; and.
FAU - Robbins, Clinton S
AU  - Robbins CS
AD  - Department of Immunology, University of Toronto, Toronto, ON, Canada; Department 
      of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, 
      Canada; and Peter Munk Cardiac Centre, Toronto General Research Institute, 
      Toronto, ON, Canada.
FAU - Paige, Christopher J
AU  - Paige CJ
AD  - Department of Immunology, University of Toronto, Toronto, ON, Canada; Ontario 
      Cancer Institute, Princess Margaret Cancer Centre, University Health Network, 
      Toronto, ON, Canada; Department of Medical Biophysics and.
LA  - eng
GR  - Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150713
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
SB  - IM
MH  - Adoptive Transfer
MH  - Animals
MH  - B-Lymphocytes/*immunology
MH  - Bone Marrow/immunology
MH  - Bone Marrow Cells/*immunology
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Flow Cytometry
MH  - Inflammation/*immunology
MH  - Lymphocyte Activation/*immunology
MH  - Lymphopoiesis/*immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
EDAT- 2015/07/15 06:00
MHDA- 2016/04/29 06:00
CRDT- 2015/07/15 06:00
PHST- 2015/03/20 00:00 [received]
PHST- 2015/07/03 00:00 [accepted]
PHST- 2015/07/15 06:00 [entrez]
PHST- 2015/07/15 06:00 [pubmed]
PHST- 2016/04/29 06:00 [medline]
AID - S0006-4971(20)30953-8 [pii]
AID - 10.1182/blood-2015-03-635805 [doi]
PST - ppublish
SO  - Blood. 2015 Sep 3;126(10):1184-92. doi: 10.1182/blood-2015-03-635805. Epub 2015 
      Jul 13.