PMID- 26170859
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150714
LR  - 20200930
IS  - 1734-1922 (Print)
IS  - 1896-9151 (Electronic)
IS  - 1734-1922 (Linking)
VI  - 11
IP  - 3
DP  - 2015 Jun 19
TI  - Influence of different intensities of vibration on proliferation and 
      differentiation of human periodontal ligament stem cells.
PG  - 638-46
LID - 10.5114/aoms.2015.52370 [doi]
AB  - INTRODUCTION: To understand the effects of low-magnitude, high-frequency (LMHF) 
      mechanical vibration at different intensities on human periodontal ligament stem 
      cell (hPDLSC) proliferation and osteogenic differentiation. MATERIAL AND METHODS: 
      The effect of vibration on hPDLSC proliferation, osteogenic differentiation, 
      tenogenic differentiation and cytoskeleton was assessed at the cellular, genetic 
      and protein level. RESULTS: The PDLSC proliferation was decreased after different 
      magnitudes of mechanical vibration; however, there were no obvious senescent 
      cells in the experimental and the static control group. Expression of 
      osteogenesis markers was increased. The expression of alkaline phosphatase (ALP) 
      and osteocalcin (OCN) mRNA was up-regulated at 0.1 g, 0.3 g, 0.6 g and 0.9 g 
      magnitude, with the peak at 0.3 g. The type I collagen (Col-I) level was 
      increased after vibration exposure at 0.1 g, 0.3 g, and 0.6 g, peaking at 0.3 g. 
      The expression levels of both mRNA and protein of Runx2 and osterix (OSX) 
      significantly increased at a magnitude of 0.1 g to 0.9 g, reached a peak at 0.3 g 
      and then decreased slowly. The scleraxis, tenogenic markers, and mRNA expression 
      decreased at 0.05 g, 0.1 g, and 0.3 g, and significantly increased at 0.6 g and 
      0.9 g. Compared with the static group, the F-actin stress fibers of hPDLSCs 
      became thicker and clearer following vibration. CONCLUSIONS: The LMHF mechanical 
      vibration promotes PDLSC osteogenic differentiation and implies the existence of 
      a magnitude-dependent effect of vibration on determining PDLSC commitment to the 
      osteoblast lineage. Changes in the cytoskeleton of hPDLSCs after vibration may be 
      one of the mechanisms of the biological effects.
FAU - Zhang, Chunxiang
AU  - Zhang C
AD  - Tianjin Stomatological Hospital of Nankai University, Tianjin, China.
FAU - Lu, Yanqin
AU  - Lu Y
AD  - Xiangya Stomatology Hospital, Central South University, Changsha, Hunan, China.
FAU - Zhang, Linkun
AU  - Zhang L
AD  - Tianjin Stomatological Hospital of Nankai University, Tianjin, China.
FAU - Liu, Yang
AU  - Liu Y
AD  - State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, 
      Sichuan University, Chengdu, China.
FAU - Zhou, Yi
AU  - Zhou Y
AD  - Affiliated Hospital of Stomatology, Medical College, Zhejiang University, 
      Hangzhou, China.
FAU - Chen, Yangxi
AU  - Chen Y
AD  - State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, 
      Sichuan University, Chengdu, China.
FAU - Yu, Haiyang
AU  - Yu H
AD  - State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, 
      Sichuan University, Chengdu, China.
LA  - eng
PT  - Journal Article
PL  - Poland
TA  - Arch Med Sci
JT  - Archives of medical science : AMS
JID - 101258257
PMC - PMC4495159
OTO - NOTNLM
OT  - magnitude
OT  - mechanical vibration
OT  - osteogenesis
OT  - periodontal ligament stem cells
OT  - proliferation
EDAT- 2015/07/15 06:00
MHDA- 2015/07/15 06:01
PMCR- 2015/06/19
CRDT- 2015/07/15 06:00
PHST- 2013/11/07 00:00 [received]
PHST- 2014/01/02 00:00 [revised]
PHST- 2014/03/06 00:00 [accepted]
PHST- 2015/07/15 06:00 [entrez]
PHST- 2015/07/15 06:00 [pubmed]
PHST- 2015/07/15 06:01 [medline]
PHST- 2015/06/19 00:00 [pmc-release]
AID - 25319 [pii]
AID - 10.5114/aoms.2015.52370 [doi]
PST - ppublish
SO  - Arch Med Sci. 2015 Jun 19;11(3):638-46. doi: 10.5114/aoms.2015.52370.