PMID- 26170978
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 10
IP  - 1
DP  - 2015 Jul
TI  - Isolation of dendritic cells from umbilical cord blood using magnetic activated 
      cell sorting or adherence.
PG  - 67-70
AB  - Dendritic cells (DCs) are a highly specialized type of antigen-presenting cell. 
      The present study describes and compares two methods for preparing DCs from 
      umbilical cord blood. The first method involves the isolation of DCs by magnetic 
      activated cell sorting (MACS). This technique isolates CD34(+) cells from cord 
      blood and induces the formation of DCs by the addition of cytokines, granulocyte 
      macrophage colony-stimulating factor and interleukin-4. The second method 
      involves the generation of large numbers of DCs from cord blood using an adherent 
      method, which isolates umbilical cord blood mononuclear cells and induces DCs in 
      the same conditions as those used in MACS. The DCs were harvested following 7 
      days of incubation and observed with an inverted microscope. The phenotype of the 
      cells was then analyzed by flow cytometry. The results revealed that, subsequent 
      to 7 days of incubation, the differentiated DCs obtained using the adherent 
      method were more mature than those isolated using MACS. However, these cells were 
      unable to be maintained in culture for more than 9-10 days. By contrast, the DCs 
      derived from CD34(+) cells by MACS were phenotypically stable and could be 
      maintained for up to 3 weeks in culture. Either method produced DCs from cord 
      blood. However, the DCs isolated using the MACS method demonstrated higher 
      homogeneity, yield and viability than those obtained using the adherent method. 
      Due to the various compositions of the monocyte subsets isolated, isolation 
      methods affect the phenotypes and functions of the resultant DCs.
FAU - Bie, Yachun
AU  - Bie Y
AD  - Department of Obstetrics and Gynecology, Beijng Chao-Yang Hospital, Capital 
      Medical University, Beijing 100020, P.R. China.
FAU - Xu, Qiuxiang
AU  - Xu Q
AD  - Department of Obstetrics and Gynecology, Beijng Chao-Yang Hospital, Capital 
      Medical University, Beijing 100020, P.R. China.
FAU - Zhang, Zhenyu
AU  - Zhang Z
AD  - Department of Obstetrics and Gynecology, Beijng Chao-Yang Hospital, Capital 
      Medical University, Beijing 100020, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20150511
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC4487079
OTO - NOTNLM
OT  - adherence
OT  - dendritic cells
OT  - magnetic activated cell sorting
OT  - umbilical cord blood mononuclear cells
EDAT- 2015/07/15 06:00
MHDA- 2015/07/15 06:01
PMCR- 2015/05/11
CRDT- 2015/07/15 06:00
PHST- 2014/06/30 00:00 [received]
PHST- 2015/03/17 00:00 [accepted]
PHST- 2015/07/15 06:00 [entrez]
PHST- 2015/07/15 06:00 [pubmed]
PHST- 2015/07/15 06:01 [medline]
PHST- 2015/05/11 00:00 [pmc-release]
AID - OL-0-0-3198 [pii]
AID - 10.3892/ol.2015.3198 [doi]
PST - ppublish
SO  - Oncol Lett. 2015 Jul;10(1):67-70. doi: 10.3892/ol.2015.3198. Epub 2015 May 11.