PMID- 26172211
OWN - NLM
STAT- MEDLINE
DCOM- 20161013
LR  - 20191210
IS  - 1751-7370 (Electronic)
IS  - 1751-7362 (Print)
IS  - 1751-7362 (Linking)
VI  - 10
IP  - 2
DP  - 2016 Feb
TI  - Linking metabolite production to taxonomic identity in environmental samples by 
      (MA)LDI-FISH.
PG  - 527-31
LID - 10.1038/ismej.2015.122 [doi]
AB  - One of the greatest challenges in microbial ecology remains to link the metabolic 
      activity of individual cells to their taxonomic identity and localization within 
      environmental samples. Here we combined mass-spectrometric imaging (MSI) through 
      (matrix-assisted) laser desorption ionization time-of-flight MSI 
      ([MA]LDI-TOF/MSI) with fluorescence in situ hybridization (FISH) to monitor 
      antibiotic production in the defensive symbiosis between beewolf wasps and 
      'Streptomyces philanthi' bacteria. Our results reveal similar distributions of 
      the different symbiont-produced antibiotics across the surface of beewolf 
      cocoons, which colocalize with the producing cell populations. Whereas FISH 
      achieves single-cell resolution, MSI is currently limited to a step size of 
      20-50 mum in the combined approach because of the destructive effects of high 
      laser intensities that are associated with tighter laser beam focus at higher 
      lateral resolution. However, on the basis of the applicability of (MA)LDI-MSI to 
      a broad range of small molecules, its combination with FISH provides a powerful 
      tool for studying microbial interactions in situ, and further modifications of 
      this technique could allow for linking metabolic profiling to gene expression.
FAU - Kaltenpoth, Martin
AU  - Kaltenpoth M
AD  - Max Planck Institute for Chemical Ecology, Research Group Insect Symbiosis, 
      Hans-Knoll-Strasse 8, Jena, Germany.
FAU - Strupat, Kerstin
AU  - Strupat K
AD  - Life Science Mass Spectrometry, Thermo Fisher Scientific, Hanna-Kunath-Strasse 
      11, Bremen, Germany.
FAU - Svatos, Ales
AU  - Svatos A
AD  - Max Planck Institute for Chemical Ecology, Research Group Mass Spectrometry, 
      Hans-Knoll-Strasse 8, Jena, Germany.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150714
PL  - England
TA  - ISME J
JT  - The ISME journal
JID - 101301086
RN  - 0 (Anti-Bacterial Agents)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/chemistry/metabolism
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Mass Spectrometry/*methods
MH  - Metabolomics/*methods
MH  - Streptomyces/chemistry/classification/*isolation & purification/*metabolism
MH  - *Symbiosis
MH  - Wasps/*microbiology/physiology
PMC - PMC4737932
EDAT- 2015/07/15 06:00
MHDA- 2016/10/14 06:00
PMCR- 2017/02/01
CRDT- 2015/07/15 06:00
PHST- 2015/02/23 00:00 [received]
PHST- 2015/05/06 00:00 [revised]
PHST- 2015/06/10 00:00 [accepted]
PHST- 2015/07/15 06:00 [entrez]
PHST- 2015/07/15 06:00 [pubmed]
PHST- 2016/10/14 06:00 [medline]
PHST- 2017/02/01 00:00 [pmc-release]
AID - ismej2015122 [pii]
AID - 10.1038/ismej.2015.122 [doi]
PST - ppublish
SO  - ISME J. 2016 Feb;10(2):527-31. doi: 10.1038/ismej.2015.122. Epub 2015 Jul 14.