PMID- 26174122
OWN - NLM
STAT- MEDLINE
DCOM- 20161215
LR  - 20161230
IS  - 1600-0625 (Electronic)
IS  - 0906-6705 (Linking)
VI  - 24
IP  - 12
DP  - 2015 Dec
TI  - 7-Hydroxydehydronuciferine induces human melanoma death via triggering autophagy 
      and apoptosis.
PG  - 930-5
LID - 10.1111/exd.12805 [doi]
AB  - Melanoma is the deadliest cancer. We identified 7-hydroxydehydronuciferine 
      (7-HDNF) isolated from the leaves of Nelumbo nucifera Gaertn cv. Rosa-plena to be 
      a bio-active agent that antagonizes melanoma tumor growth in mice xenograft model 
      in vivo. Cell proliferation assay demonstrated strong anticancer effects of 
      7-HDNF to exhibit a dose-dependent behaviour and displayed minor cytotoxicities 
      on normal human skin cells, including epidermal keratinocytes and melanocytes, 
      and dermal fibroblasts. With acridine orange (AO) staining and flow analysis, we 
      found 7-HDNF induced the formation of intracellular vacuoles and the augmentation 
      of acidic vesicular organelles (AVO). The apoptotic cell death ratio was measured 
      via two-dimensional flow cytometry by annexin V-fluorescein isothiocyanate 
      (FITC)/propidium iodide (PI) double stained to confirm the cellular membrane 
      asymmetry lost. One-dimensional flow cytometric analysis showed 7-HDNF increased 
      the cellular arrest in cell cycle at the G2/M phase. Through Western blot 
      examinations, protein expressions were discovered to verify autophagy and 
      apoptosis response mechanisms sharing the associated pathways. Finally, 7-HDNF 
      presented a high-quality antimigratory activity in wound-healing assay. Overall, 
      7-HDNF presented high-quality anticancer bio-functions and inhibited melanoma 
      tumor growth in vivo and in vitro.
CI  - (c) 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Wu, Pei-Fang
AU  - Wu PF
AD  - Department of Fragrance and Cosmetic Science, Kaohsiung Medical University, 
      Kaohsiung, Taiwan, ROC.
FAU - Chiu, Chien-Chih
AU  - Chiu CC
AD  - Department of Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan, 
      ROC.
FAU - Chen, Chung-Yi
AU  - Chen CY
AD  - School of Medical and Health Sciences, Fooyin University, Kaohsiung, Taiwan, ROC.
FAU - Wang, Hui-Min David
AU  - Wang HM
AD  - Department of Fragrance and Cosmetic Science, Kaohsiung Medical University, 
      Kaohsiung, Taiwan, ROC.
AD  - Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung, 
      Taiwan, ROC.
AD  - Center for Stem Cell Research, Kaohsiung Medical University, Kaohsiung, Taiwan, 
      ROC.
AD  - Department of Marine Biotechnology and Resources, National Sun Yat-Sen 
      University, Kaohsiung, Taiwan, ROC.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150821
PL  - Denmark
TA  - Exp Dermatol
JT  - Experimental dermatology
JID - 9301549
RN  - 0 (7-hydroxydehydronuciferine)
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Aporphines)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Mitochondrial Proteins)
RN  - W26UEB90B7 (nuciferine)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents, Phytogenic/chemistry/*pharmacology
MH  - Apoptosis/drug effects
MH  - Aporphines/chemistry/*pharmacology
MH  - Autophagy/drug effects
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Drugs, Chinese Herbal/chemistry/pharmacology
MH  - Female
MH  - G2 Phase Cell Cycle Checkpoints/drug effects
MH  - Humans
MH  - Keratinocytes/cytology/drug effects
MH  - Medicine, Chinese Traditional
MH  - Melanoma/*drug therapy/metabolism/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Microtubule-Associated Proteins/metabolism
MH  - Mitochondrial Proteins/metabolism
MH  - Nelumbo/chemistry
MH  - Plants, Medicinal/chemistry
MH  - Xenograft Model Antitumor Assays
OTO - NOTNLM
OT  - 7-hydroxydehydronuciferine
OT  - apoptosis
OT  - autophagy
OT  - melanoma
OT  - metastasis
EDAT- 2015/07/16 06:00
MHDA- 2016/12/16 06:00
CRDT- 2015/07/16 06:00
PHST- 2015/07/07 00:00 [accepted]
PHST- 2015/07/16 06:00 [entrez]
PHST- 2015/07/16 06:00 [pubmed]
PHST- 2016/12/16 06:00 [medline]
AID - 10.1111/exd.12805 [doi]
PST - ppublish
SO  - Exp Dermatol. 2015 Dec;24(12):930-5. doi: 10.1111/exd.12805. Epub 2015 Aug 21.