PMID- 26176550
OWN - NLM
STAT- MEDLINE
DCOM- 20160603
LR  - 20211203
IS  - 1557-8666 (Electronic)
IS  - 1066-5277 (Print)
IS  - 1066-5277 (Linking)
VI  - 22
IP  - 9
DP  - 2015 Sep
TI  - Defining the Domain Arrangement of the Mammalian Target of Rapamycin Complex 
      Component Rictor Protein.
PG  - 876-86
LID - 10.1089/cmb.2015.0103 [doi]
AB  - Mammalian target of rapamycin (mTOR) complexes play a pivotal role in the cell. 
      Raptor and Rictor proteins interact with mTOR to form two distinct complexes, 
      mTORC1 and mTORC2, respectively. While the domain structure of Raptor is known, 
      current bioinformatics tools failed to classify the domains in Rictor. Here we 
      focus on identifying specific domains in Rictor by searching for conserved 
      regions. We scanned the pdb structural database and constructed three protein 
      domain datasets. Next we carried out multiple pairwise sequence alignments of the 
      proteins in the domain dataset. By analyzing the z-scores of Rictor sequence 
      similarity to protein sequences in the dataset, we assigned the structural and 
      functional domains of Rictor. We found that, like Raptor, Rictor also has HEAT 
      and WD40 domains, which could be the common motif binding to mTORC. Rictor may 
      also have pleckstrin homology domains, which mediate cellular localization and 
      transmit signals to downstream targets, as well as a domain that is homologous to 
      50S protein L17 and human 39S protein L17. This putative ribosome binding domain 
      could mediate mTORC2-ribosome interaction.
FAU - Zhou, Ping
AU  - Zhou P
AD  - 1 Research Center of Basic Medical Sciences and Cancer Institute and Hospital, 
      Tianjin Medical University , Tianjin, China .
FAU - Zhang, Ning
AU  - Zhang N
AD  - 1 Research Center of Basic Medical Sciences and Cancer Institute and Hospital, 
      Tianjin Medical University , Tianjin, China .
FAU - Nussinov, Ruth
AU  - Nussinov R
AD  - 2 Basic Science Program, Leidos Biomedical Research, Inc., Cancer and 
      Inflammation Program, National Cancer Institute , Frederick, Maryland.
AD  - 3 Department of Human Genetics and Molecular Medicine, Sackler Institute of 
      Molecular Medicine, Sackler School of Medicine, Tel Aviv University , Tel Aviv, 
      Israel .
FAU - Ma, Buyong
AU  - Ma B
AD  - 2 Basic Science Program, Leidos Biomedical Research, Inc., Cancer and 
      Inflammation Program, National Cancer Institute , Frederick, Maryland.
LA  - eng
GR  - Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, N.I.H., Intramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20150715
PL  - United States
TA  - J Comput Biol
JT  - Journal of computational biology : a journal of computational molecular cell 
      biology
JID - 9433358
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Carrier Proteins)
RN  - 0 (Multiprotein Complexes)
RN  - 0 (RICTOR protein, human)
RN  - 0 (RPTOR protein, human)
RN  - 0 (Rapamycin-Insensitive Companion of mTOR Protein)
RN  - 0 (Regulatory-Associated Protein of mTOR)
RN  - EC 2.7.11.1 (Mechanistic Target of Rapamycin Complex 1)
RN  - EC 2.7.11.1 (Mechanistic Target of Rapamycin Complex 2)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/metabolism
MH  - Amino Acid Sequence
MH  - Carrier Proteins/*metabolism
MH  - Computational Biology/methods
MH  - Conserved Sequence/genetics
MH  - Humans
MH  - Mechanistic Target of Rapamycin Complex 1
MH  - Mechanistic Target of Rapamycin Complex 2
MH  - Multiprotein Complexes/*metabolism
MH  - Protein Binding/genetics
MH  - Protein Interaction Domains and Motifs/genetics
MH  - Protein Structure, Tertiary/genetics
MH  - Rapamycin-Insensitive Companion of mTOR Protein
MH  - Regulatory-Associated Protein of mTOR
MH  - Ribosomes
MH  - Sequence Alignment/methods
MH  - Signal Transduction/genetics
MH  - TOR Serine-Threonine Kinases/*metabolism
PMC - PMC4575542
OTO - NOTNLM
OT  - Raptor
OT  - Rictor
OT  - mTOR
OT  - protein domain
OT  - protein-protein interactions
EDAT- 2015/07/16 06:00
MHDA- 2016/06/04 06:00
PMCR- 2016/09/01
CRDT- 2015/07/16 06:00
PHST- 2015/07/16 06:00 [entrez]
PHST- 2015/07/16 06:00 [pubmed]
PHST- 2016/06/04 06:00 [medline]
PHST- 2016/09/01 00:00 [pmc-release]
AID - 10.1089/cmb.2015.0103 [pii]
AID - 10.1089/cmb.2015.0103 [doi]
PST - ppublish
SO  - J Comput Biol. 2015 Sep;22(9):876-86. doi: 10.1089/cmb.2015.0103. Epub 2015 Jul 
      15.