PMID- 26178669
OWN - NLM
STAT- MEDLINE
DCOM- 20160620
LR  - 20150919
IS  - 1931-8405 (Electronic)
IS  - 0889-2229 (Linking)
VI  - 31
IP  - 10
DP  - 2015 Oct
TI  - Baculovirus Infection of Human Monocyte-Derived Dendritic Cells Restricts HIV-1 
      Replication.
PG  - 1023-31
LID - 10.1089/AID.2015.0060 [doi]
AB  - Acquired immune deficiency syndrome (AIDS) is mainly caused by infection with 
      human immunodeficiency virus-1 (HIV-1) and still poses a global threat for which 
      we lack a protective or therapeutic vaccine. Dendritic cells (DCs) play a major 
      role in the onset of HIV infection, providing one of the primary sites of HIV 
      replication, and also act as viral reservoirs in vivo. Previous studies have 
      shown that baculovirus (BV) induces strong host immune responses against 
      infections and malignancies. In this study, we infected human monocyte-derived 
      DCs with recombinant BV (AcCAG-gag) and showed that AcCAG-gag-infected human DCs 
      underwent maturation and produced interferon alpha and other proinflammatory 
      cytokines accompanied by increases in the mRNA and protein expression levels of 
      APOBEC3 (A3A, A3F, and A3G), proteins associated with the inhibition of HIV-1 
      replication. Surprisingly, HIV-1 inhibition is also observed in human DCs 
      infected with a wild-type BV, as determined by the production of inflammatory 
      cytokines, the expression of A3, and a reduction in the p24 level. Our findings 
      outline the mechanism underlying the inhibition of HIV-1 in BV-infected human DCs 
      and pave the way for the use of BV as an effective tool for immunotherapy against 
      HIV-1.
FAU - Chang, Myint Oo
AU  - Chang MO
AD  - 1 High Technology Research Centre, Chiba Institute of Technology , Chiba, Japan .
FAU - Suzuki, Tomoyuki
AU  - Suzuki T
AD  - 2 Department of Life and Environmental Sciences, Chiba Institute of Technology , 
      Chiba, Japan .
FAU - Kitajima, Masayuki
AU  - Kitajima M
AD  - 2 Department of Life and Environmental Sciences, Chiba Institute of Technology , 
      Chiba, Japan .
AD  - 3 Department of Immunology and Pathology, Research Institute National Center for 
      Global Health and Medicine , Chiba, Japan .
FAU - Takaku, Hiroshi
AU  - Takaku H
AD  - 1 High Technology Research Centre, Chiba Institute of Technology , Chiba, Japan .
AD  - 2 Department of Life and Environmental Sciences, Chiba Institute of Technology , 
      Chiba, Japan .
AD  - 4 Research Institute, Chiba Institute of Technology , Chiba, Japan .
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150806
PL  - United States
TA  - AIDS Res Hum Retroviruses
JT  - AIDS research and human retroviruses
JID - 8709376
SB  - IM
MH  - Baculoviridae/*growth & development
MH  - Cells, Cultured
MH  - Dendritic Cells/*virology
MH  - HIV-1/*growth & development
MH  - Humans
MH  - *Immunity, Innate
MH  - *Viral Interference
EDAT- 2015/07/17 06:00
MHDA- 2016/06/21 06:00
CRDT- 2015/07/17 06:00
PHST- 2015/07/17 06:00 [entrez]
PHST- 2015/07/17 06:00 [pubmed]
PHST- 2016/06/21 06:00 [medline]
AID - 10.1089/AID.2015.0060 [doi]
PST - ppublish
SO  - AIDS Res Hum Retroviruses. 2015 Oct;31(10):1023-31. doi: 10.1089/AID.2015.0060. 
      Epub 2015 Aug 6.