PMID- 26178695
OWN - NLM
STAT- MEDLINE
DCOM- 20160711
LR  - 20181113
IS  - 1613-4133 (Electronic)
IS  - 1613-4125 (Print)
IS  - 1613-4125 (Linking)
VI  - 59
IP  - 10
DP  - 2015 Oct
TI  - Cor a 14, the allergenic 2S albumin from hazelnut, is highly thermostable and 
      resistant to gastrointestinal digestion.
PG  - 2077-86
LID - 10.1002/mnfr.201500071 [doi]
AB  - SCOPE: Allergens from nuts frequently induce severe allergic reactions in 
      sensitive individuals. The aim of this study was to elucidate the physicochemical 
      characteristics of natural Cor a 14, the 2S albumin from hazelnut. METHODS AND 
      RESULTS: Cor a 14 was purified from raw hazelnuts using a combination of 
      precipitation and chromatographic techniques. The protein was analyzed using gel 
      electrophoresis, MS, and far-UV circular dichroism (CD) analyses. The 
      immunoglobulin E (IgE) binding of native, heat-treated, and in vitro digested Cor 
      a 14 was studied. We identified two different Cor a 14 isoforms and showed 
      microclipping at the C-terminus. CD spectra at room temperature showed the 
      typical characteristics of 2S albumins, and temperatures of more than 80 degrees C were 
      required to start unfolding of Cor a 14 demonstrating its high stability to heat 
      treatment. In vitro digestion experiments revealed that Cor a 14 is resistant to 
      proteolytic degradation. Native and heat-treated protein was recognized by sera 
      from hazelnut allergic patients. However, denaturation of the allergen led to 
      significantly reduced IgE binding. CONCLUSION: We identified two different 
      isoforms of Cor a 14 displaying high stability under heating and gastric and 
      duodenal conditions. Data from IgE-binding experiments revealed the existence of 
      both, linear and conformational epitopes.
CI  - (c) 2015 The Authors. Molecular Nutrition & Food Research published by Wiley-VCH 
      Verlag GmbH & Co. KGaA, Weinheim.
FAU - Pfeifer, Sabine
AU  - Pfeifer S
AD  - Department of Pathophysiology and Allergy Research, Center for Pathophysiology, 
      Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
FAU - Bublin, Merima
AU  - Bublin M
AD  - Department of Pathophysiology and Allergy Research, Center for Pathophysiology, 
      Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
FAU - Dubiela, Pawel
AU  - Dubiela P
AD  - Department of Pathophysiology and Allergy Research, Center for Pathophysiology, 
      Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
FAU - Hummel, Karin
AU  - Hummel K
AD  - VetCore Facility, University of Veterinary Medicine, Vienna, Austria.
FAU - Wortmann, Judith
AU  - Wortmann J
AD  - Institute of Molecular Biosciences, Structural Biology, University of Graz, Graz, 
      Austria.
FAU - Hofer, Gerhard
AU  - Hofer G
AD  - Institute of Molecular Biosciences, Structural Biology, University of Graz, Graz, 
      Austria.
FAU - Keller, Walter
AU  - Keller W
AD  - Institute of Molecular Biosciences, Structural Biology, University of Graz, Graz, 
      Austria.
FAU - Radauer, Christian
AU  - Radauer C
AD  - Department of Pathophysiology and Allergy Research, Center for Pathophysiology, 
      Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
FAU - Hoffmann-Sommergruber, Karin
AU  - Hoffmann-Sommergruber K
AD  - Department of Pathophysiology and Allergy Research, Center for Pathophysiology, 
      Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150806
PL  - Germany
TA  - Mol Nutr Food Res
JT  - Molecular nutrition & food research
JID - 101231818
RN  - 0 (Allergens)
RN  - 0 (Antigens, Plant)
RN  - 0 (Cor a 14 allergen, hazelnut)
RN  - 0 (Protein Isoforms)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*chemistry/isolation & purification/*metabolism
MH  - Amino Acid Sequence
MH  - Antigens, Plant/*chemistry/isolation & purification/*metabolism
MH  - *Digestion
MH  - Hot Temperature
MH  - Humans
MH  - Immunoglobulin E/metabolism
MH  - Molecular Sequence Data
MH  - Protein Isoforms/chemistry/metabolism
MH  - Protein Stability
MH  - Protein Structure, Secondary
MH  - Proteolysis
PMC - PMC4973671
OTO - NOTNLM
OT  - 2S albumin
OT  - Allergen
OT  - Cor a 14
OT  - Food allergy
OT  - Hazelnut (Corylus avellana)
EDAT- 2015/07/17 06:00
MHDA- 2016/07/12 06:00
PMCR- 2016/08/04
CRDT- 2015/07/17 06:00
PHST- 2015/01/29 00:00 [received]
PHST- 2015/05/07 00:00 [revised]
PHST- 2015/06/25 00:00 [accepted]
PHST- 2015/07/17 06:00 [entrez]
PHST- 2015/07/17 06:00 [pubmed]
PHST- 2016/07/12 06:00 [medline]
PHST- 2016/08/04 00:00 [pmc-release]
AID - MNFR2442 [pii]
AID - 10.1002/mnfr.201500071 [doi]
PST - ppublish
SO  - Mol Nutr Food Res. 2015 Oct;59(10):2077-86. doi: 10.1002/mnfr.201500071. Epub 
      2015 Aug 6.