PMID- 26178696
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1522-2683 (Electronic)
IS  - 0173-0835 (Linking)
VI  - 36
IP  - 21-22
DP  - 2015 Nov
TI  - On-line concentration and pressurized capillary electrochromatography analysis of 
      five beta-agonists in human urine using a methacrylate monolithic column.
PG  - 2720-2726
LID - 10.1002/elps.201500229 [doi]
AB  - A pressurized capillary electrochromatography (pCEC) method combined with an 
      online concentration was developed for the separation and determination of five 
      beta(2) -blockers (terbutaline, salbutamol, formoterol, procaterol and salmeterol) 
      in human urine. A stearyl methacrylate-based monolithic column was prepared as 
      the separation column. The various separation parameters including acetontrile 
      concentration, applied voltage, pH and concentration of the running buffer were 
      investigated. On-line concentration methods in combination of the chromatographic 
      zone-sharpening effect and field-enhanced sample-stacking effect were utilized to 
      improve detection sensitivity. The on-line concentration parameters including 
      injection voltage, injection time, as well as sample matrix were systematically 
      studied. Compared with the conventional sample injection, the on-line 
      concentration technique appeared to improve the corresponding sensitivities 
      20 reverse similar50-fold. Furthermore, good precision was obtained with relative standard 
      deviations (RSDs) for retention times within 1.09% and for peak areas less than 
      3.55% (n = 5). The established method was successfully applied to the 
      determination of above-mentioned beta(2) -agonists in urine samples. The recoveries 
      of spiked urine samples were between 85.0 and 113.3% with RSDs less than 5.39%.
CI  - (c) 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Chen, Zongbao
AU  - Chen Z
AD  - Key Laboratory of Applied Organic Chemistry, College of Jiangxi Province 
      Department of Chemistry, Shangrao Normal University, Jiangxi, P. R., China.
AD  - Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, 
      Department of Chemistry, Fuzhou University, Fuzhou, Fujian, P. R., China.
FAU - Zhang, Lan
AU  - Zhang L
AD  - Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, 
      Department of Chemistry, Fuzhou University, Fuzhou, Fujian, P. R., China.
FAU - Lu, Qiaomei
AU  - Lu Q
AD  - Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, 
      Department of Chemistry, Fuzhou University, Fuzhou, Fujian, P. R., China.
FAU - Ye, Qing
AU  - Ye Q
AD  - Key Laboratory of Applied Organic Chemistry, College of Jiangxi Province 
      Department of Chemistry, Shangrao Normal University, Jiangxi, P. R., China.
FAU - Zhang, Lin
AU  - Zhang L
AD  - Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, 
      Department of Chemistry, Fuzhou University, Fuzhou, Fujian, P. R., China.
LA  - eng
PT  - Journal Article
DEP - 20150907
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
OTO - NOTNLM
OT  - Monolithic column
OT  - On-line concentration
OT  - Pressurized capillary electrochromatography
OT  - beta2-Agonists
EDAT- 2015/07/17 06:00
MHDA- 2015/07/17 06:01
CRDT- 2015/07/17 06:00
PHST- 2015/01/30 00:00 [received]
PHST- 2015/06/13 00:00 [revised]
PHST- 2015/06/29 00:00 [accepted]
PHST- 2015/07/17 06:00 [pubmed]
PHST- 2015/07/17 06:01 [medline]
PHST- 2015/07/17 06:00 [entrez]
AID - 10.1002/elps.201500229 [doi]
PST - ppublish
SO  - Electrophoresis. 2015 Nov;36(21-22):2720-2726. doi: 10.1002/elps.201500229. Epub 
      2015 Sep 7.