PMID- 26179065
OWN - NLM
STAT- MEDLINE
DCOM- 20160211
LR  - 20181202
IS  - 1472-6882 (Electronic)
IS  - 1472-6882 (Linking)
VI  - 15
DP  - 2015 Jul 16
TI  - Effects of kolaviron on hepatic oxidative stress in streptozotocin induced 
      diabetes.
PG  - 236
LID - 10.1186/s12906-015-0760-y [doi]
LID - 236
AB  - BACKGROUND: Alteration in antioxidant defence and increase in oxidative stress 
      that results in tissue injury is characteristic of diabetes. We evaluated the 
      protective effects of kolaviron (a flavonoid complex extracted from the seeds of 
      Garcinia kola) on hepatic antioxidants, lipid peroxidation and apoptosis in 
      diabetic rats. METHODS: To induce diabetes, rats were injected with 
      streptozotocin intraperitoneally at a single dose of 50 mg/kg. Kolaviron 
      (100 mg/kg) was administered orally for 6 weeks (5 times weekly). Activities of 
      liver antioxidant enzymes was analysed with Multiskan Spectrum plate reader. High 
      performance liquid chromatography (HPLC) was used in the analysis of MDA 
      (malondialdehyde), a product of lipid peroxidation. Apoptosis was assessed by 
      terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) 
      assay. RESULT: Diabetic rats exhibited a significant increase in the peroxidation 
      of hepatic lipids as observed from the elevated level of malondialdehyde (MDA). 
      In addition, Oxygen Radical Absorbance Capacity (ORAC), level of reduced 
      glutathione (GSH), ratio of reduced to oxidized glutathione (GSH: GSSG) and 
      catalase (CAT) activity were decreased in the liver of diabetic rats. The 
      activities of GPX (glutathione peroxidase) and SOD (superoxide dismutase) were 
      unaltered in diabetic rats. TUNEL assay revealed increased apoptotic cell death 
      in the liver. Kolaviron attenuated lipid peroxidation and apoptosis, increased 
      CAT activity, GSH levels and GSH: GSSG ratio. The ORAC of kolaviron-treated 
      diabetic liver was restored to near-normal values. CONCLUSION: Kolaviron protects 
      the liver against oxidative and apoptotic damage induced by hyperglycemia.
FAU - Oyenihi, Omolola R
AU  - Oyenihi OR
AD  - Department of Biochemistry, Bowen University, Iwo, Nigeria.
FAU - Brooks, Nicole L
AU  - Brooks NL
AD  - Department of Wellness Sciences, Cape Peninsula University of Technology, Cape 
      Town, South Africa.
FAU - Oguntibeju, Oluwafemi O
AU  - Oguntibeju OO
AD  - Nutrition and Chronic Diseases Unit, Oxidative Stress Research Centre, Department 
      of Biomedical Sciences, Cape Peninsula University of Technology, Bellville, South 
      Africa. oguntibejuo@cput.ac.za.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150716
PL  - England
TA  - BMC Complement Altern Med
JT  - BMC complementary and alternative medicine
JID - 101088661
RN  - 0 (Antioxidants)
RN  - 0 (Flavonoids)
RN  - 0 (Plant Extracts)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - GAN16C9B8O (Glutathione)
RN  - PX7M0YV62G (kolaviron)
SB  - IM
MH  - Animals
MH  - Antioxidants/metabolism/pharmacology/*therapeutic use
MH  - Apoptosis/drug effects
MH  - Catalase/metabolism
MH  - Diabetes Mellitus, Experimental/*drug therapy/metabolism/pathology
MH  - Flavonoids/pharmacology/*therapeutic use
MH  - Garcinia kola/*chemistry
MH  - Glutathione/metabolism
MH  - Glutathione Peroxidase/metabolism
MH  - Hyperglycemia/drug therapy
MH  - Lipid Peroxidation/drug effects
MH  - Liver/*drug effects/metabolism/pathology
MH  - Male
MH  - Malondialdehyde/metabolism
MH  - Oxidative Stress/*drug effects
MH  - *Phytotherapy
MH  - Plant Extracts/pharmacology/therapeutic use
MH  - Rats
MH  - Rats, Wistar
MH  - Seeds/chemistry
MH  - Superoxide Dismutase/metabolism
PMC - PMC4502612
EDAT- 2015/07/17 06:00
MHDA- 2016/02/13 06:00
PMCR- 2015/07/16
CRDT- 2015/07/17 06:00
PHST- 2015/01/30 00:00 [received]
PHST- 2015/07/02 00:00 [accepted]
PHST- 2015/07/17 06:00 [entrez]
PHST- 2015/07/17 06:00 [pubmed]
PHST- 2016/02/13 06:00 [medline]
PHST- 2015/07/16 00:00 [pmc-release]
AID - 10.1186/s12906-015-0760-y [pii]
AID - 760 [pii]
AID - 10.1186/s12906-015-0760-y [doi]
PST - epublish
SO  - BMC Complement Altern Med. 2015 Jul 16;15:236. doi: 10.1186/s12906-015-0760-y.