PMID- 26182477
OWN - NLM
STAT- MEDLINE
DCOM- 20150901
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 33
IP  - 3
DP  - 2015 Mar
TI  - [Simultaneous determination of five hypertoxic rodenticides in serum by gas 
      chromatography-mass spectrometry].
PG  - 323-8
AB  - A fast analytical method based on gas chromatography-mass spectrometry (GC-MS) 
      was established for the simultaneous determination of tetramine, fluoroacetamide, 
      sodium fluoroacetate, gliftor I and gliftor II in serum. At pH 2.0, sodium 
      fluoroacetate was derivatizated at room temperature for 5 min by using 
      N,N-diethyl-p-phenylenediamine as the derivatization reagent and N, 
      N'-dicyclohexylcarbodiimide as the catalyst. The derivative and other 
      rodenticides were extracted with ethyl acetate and concentrated with nitrogen at 
      50  degrees C, then determined by GC-MS in selected ion monitoring (SIM) mode, and 
      quantified with matrix-match standard solutions. The analysis was carried out on 
      an ionic liquid chromatographic capillary column (SLB-IL59, 30 m x 0.25 mm x 0.20 
      microm, maximum temperature 300  degrees C) at a flow rate of 1.0 mL/min, and the five 
      rodenticides were successfully separated in 15 min when temperature programming 
      was used. The results showed that the calibration curves were linear in the range 
      of 0.01-1.0 mg/L, except for fluoroacetamide (0.02-2.0 mg/L) and tetramine 
      (0.02-10 mg/L), with correlation coefficients (R2) greater than 0.995, and the 
      limits of detection (LODs) were 0.001-0.002 mg/L (S/N = 3). The recoveries were 
      84.0%-110.0% at three different spiked levels, and the relative standard 
      deviations (RSDs) were 2.9%-7.5% (n = 6). The method is simple, accurate, highly 
      sensitive and suitable for the detection of the five hypertoxic rodenticides in 
      serum for toxicological purposes.
FAU - Huang, Huiqiu
AU  - Huang H
FAU - Huang, Xun
AU  - Huang X
FAU - Yu, Jingsun
AU  - Yu J
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Bridged-Ring Compounds)
RN  - 0 (Drug Combinations)
RN  - 0 (Fluoroacetates)
RN  - 0 (Phenylenediamines)
RN  - 0 (Propanols)
RN  - 0 (Rodenticides)
RN  - 8065-71-2 (gliftor)
RN  - B18R611M38 (fluoroacetamide)
RN  - F6TS3WME05 (tetramethylenedisulfotetramine)
RN  - U770QIT64J (4-phenylenediamine)
SB  - IM
MH  - Bridged-Ring Compounds
MH  - Drug Combinations
MH  - Fluoroacetates
MH  - *Gas Chromatography-Mass Spectrometry
MH  - Phenylenediamines
MH  - Propanols
MH  - Rodenticides/*blood
EDAT- 2015/07/18 06:00
MHDA- 2015/09/02 06:00
CRDT- 2015/07/18 06:00
PHST- 2015/07/18 06:00 [entrez]
PHST- 2015/07/18 06:00 [pubmed]
PHST- 2015/09/02 06:00 [medline]
AID - 10.3724/sp.j.1123.2014.11024 [doi]
PST - ppublish
SO  - Se Pu. 2015 Mar;33(3):323-8. doi: 10.3724/sp.j.1123.2014.11024.