PMID- 26184188
OWN - NLM
STAT- MEDLINE
DCOM- 20160419
LR  - 20181113
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 16
IP  - 7
DP  - 2015 Jul 15
TI  - Identification of Rhopalosiphum Padi Virus 5' Untranslated Region Sequences 
      Required for Cryptic Promoter Activity and Internal Ribosome Entry.
PG  - 16053-66
LID - 10.3390/ijms160716053 [doi]
AB  - The 579-nucleotide 5' untranslated region (5'UTR) of the Rhopalosiphum padi virus 
      (RhPV) possesses a cross-kingdom internal ribosome entry site (IRES) activity 
      that functions in insect, mammalian, and plant-derived in vitro translation 
      systems, and six TAAG motifs within the DNA fragment encoding the RhPV 5'UTR were 
      previously found to confer the RhPV 5'UTR with late promoter activity in 
      baculovirus. In the present study, various truncated RhPV 5'UTR sequences were 
      produced, and among them, a fragment of 110 bp ranging from nucleotides 309 to 
      418 was identified to be the shortest fragment responsible for the late promoter 
      activity in baculovirus infected Sf21 cells. This 110 bp fragment contains a TAAG 
      tandem repeat that retains more than 60% of the late promoter activity of the 
      full length RhPV 5'UTR sequence. Further, IRES activity remained unchanged in all 
      truncated RhPV 5'UTR constructs. Taken together, this novel 110 bp fragment 
      having late promoter activity in baculovirus as well as IRES activity in 
      mammalian cell, renders it a useful tool for the development of a "shuttle" 
      bi-cistronic baculovirus gene expression and/or delivery vector.
FAU - Liu, Ming-Kun
AU  - Liu MK
AD  - Institute of Bioinformatics and Structural Biology, National Tsing Hua 
      University, Hsinchu 300, Taiwan. lmk33@hotmail.com.
FAU - Lin, Jie-Zue
AU  - Lin JZ
AD  - Institute of Bioinformatics and Structural Biology, National Tsing Hua 
      University, Hsinchu 300, Taiwan. blackeye514@hotmail.com.
FAU - Jinn, Tzyy-Rong
AU  - Jinn TR
AD  - School of Chinese Medicine, China Medical University, Taichung 40402, Taiwan. 
      jinn@mail.cmu.edu.tw.
FAU - Chan, Hong-Lin
AU  - Chan HL
AD  - Institute of Bioinformatics and Structural Biology, National Tsing Hua 
      University, Hsinchu 300, Taiwan. hlchan@life.nthu.edu.tw.
FAU - Wu, Tzong-Yuan
AU  - Wu TY
AD  - Department of Bioscience Technology, Chung Yuan Christian University, Chungli 
      320, Taiwan. tywu@cycu.edu.tw.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150715
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (5' Untranslated Regions)
SB  - IM
MH  - 5' Untranslated Regions
MH  - Animals
MH  - Baculoviridae/*genetics
MH  - CHO Cells
MH  - Cricetinae
MH  - Cricetulus
MH  - Genes, Viral
MH  - Genetic Vectors/genetics/metabolism
MH  - Promoter Regions, Genetic
MH  - Ribosomes/*metabolism
MH  - Sf9 Cells
MH  - Spodoptera
PMC - PMC4519938
OTO - NOTNLM
OT  - IRES
OT  - RhPV
OT  - baculovirus
OT  - cryptic promoter
OT  - dicistroviruses
EDAT- 2015/07/18 06:00
MHDA- 2016/04/20 06:00
PMCR- 2015/07/01
CRDT- 2015/07/18 06:00
PHST- 2015/04/04 00:00 [received]
PHST- 2015/06/15 00:00 [revised]
PHST- 2015/07/02 00:00 [accepted]
PHST- 2015/07/18 06:00 [entrez]
PHST- 2015/07/18 06:00 [pubmed]
PHST- 2016/04/20 06:00 [medline]
PHST- 2015/07/01 00:00 [pmc-release]
AID - ijms160716053 [pii]
AID - ijms-16-16053 [pii]
AID - 10.3390/ijms160716053 [doi]
PST - epublish
SO  - Int J Mol Sci. 2015 Jul 15;16(7):16053-66. doi: 10.3390/ijms160716053.