PMID- 26187161
OWN - NLM
STAT- MEDLINE
DCOM- 20160525
LR  - 20150831
IS  - 1096-1208 (Electronic)
IS  - 0882-4010 (Linking)
VI  - 87
DP  - 2015 Oct
TI  - Respiratory and neurological disease in rabbits experimentally infected with 
      equid herpesvirus 1.
PG  - 45-50
LID - S0882-4010(15)00107-2 [pii]
LID - 10.1016/j.micpath.2015.07.007 [doi]
AB  - Equid herpesvirus type 1 (EHV-1) is an important pathogen of horses worldwide, 
      associated with respiratory, reproductive and/or neurological disease. A mouse 
      model for EHV-1 infection has been established but fails to reproduce some 
      important aspects of the viral pathogenesis. Then, we investigated the 
      susceptibility of rabbits to EHV-1 aiming at proposing this species as an 
      alternative model for EHV-1 infection. Weanling rabbits inoculated intranasal 
      with EHV-1 Kentucky D (10(7) TCID50/animal) shed virus in nasal secretions up to 
      day 8-10 post-inoculation (pi), presented viremia up to day 14 pi and 
      seroconverted to EHV-1 (virus neutralizing titers 4 to 64). Most rabbits (75%) 
      developed respiratory disease, characterized by serous to hemorrhagic nasal 
      discharge and mild to severe dyspnea. Some animals (20%) presented neurological 
      signs as circling, bruxism and opisthotonus. Six animals died during acute 
      disease (days 3-6); infectious virus and/or viral DNA were detected in the lungs, 
      trigeminal ganglia (TG), olfactory bulbs (OBs) and cerebral cortex/brain (CC). 
      Histological examination showed necrohemorrhagic, multifocal to coalescent 
      bronchointerstitial pneumonia and diffuse alveolar edema. In two rabbits 
      euthanized at day 50 pi, latent EHV-1 DNA was detected in the OBs. Dexamethasone 
      administration at day 50 pi resulted in virus reactivation, demonstrated by virus 
      shedding, viremia, clinical signs, and increase in VN titers and/or by detection 
      of virus DNA in lungs, OBs, TGs and/or CC. These results demonstrate that rabbits 
      are susceptible to EHV-1 infection and develop respiratory and neurological signs 
      upon experimental inoculation. Thus, rabbits may be used to study selected 
      aspects of EHV-1 biology and pathogenesis, extending and complementing the mouse 
      model.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Kanitz, Fabio A
AU  - Kanitz FA
AD  - Setor de Virologia, Departamento de Medicina Veterinaria Preventiva, Universidade 
      Federal de Santa Maria, Av. Roraima, 1000, predio 20, sala 4200, Santa Maria, RS, 
      CEP 97105-900, Brazil; 9(a) Supervisao Regional da Secretaria da Agricultura e 
      Pecuaria do Rio Grande do Sul, Rua Mario Santo Dani, 100, Osorio, RS, CEP 
      95520-000, Brazil.
FAU - Cargnelutti, Juliana F
AU  - Cargnelutti JF
AD  - Setor de Virologia, Departamento de Medicina Veterinaria Preventiva, Universidade 
      Federal de Santa Maria, Av. Roraima, 1000, predio 20, sala 4200, Santa Maria, RS, 
      CEP 97105-900, Brazil.
FAU - Anziliero, Deniz
AU  - Anziliero D
AD  - Setor de Virologia, Departamento de Medicina Veterinaria Preventiva, Universidade 
      Federal de Santa Maria, Av. Roraima, 1000, predio 20, sala 4200, Santa Maria, RS, 
      CEP 97105-900, Brazil; Escola de Medicina, Faculdade Meridional - IMED, Senador 
      Pinheiro, 304, Passo Fundo, RS, CEP 99070-220, Brazil.
FAU - Goncalves, Kelley V
AU  - Goncalves KV
AD  - Setor de Virologia, Departamento de Medicina Veterinaria Preventiva, Universidade 
      Federal de Santa Maria, Av. Roraima, 1000, predio 20, sala 4200, Santa Maria, RS, 
      CEP 97105-900, Brazil.
FAU - Masuda, Eduardo K
AU  - Masuda EK
AD  - Axys Analises Laboratorio Veterinario e Consultoria Diagnostica, Rua Pedro Chaves 
      Barcelos, 475, Bairro Montserrat, Porto Alegre, RS, CEP 90450-010, Brazil.
FAU - Weiblen, Rudi
AU  - Weiblen R
AD  - Setor de Virologia, Departamento de Medicina Veterinaria Preventiva, Universidade 
      Federal de Santa Maria, Av. Roraima, 1000, predio 20, sala 4200, Santa Maria, RS, 
      CEP 97105-900, Brazil.
FAU - Flores, Eduardo F
AU  - Flores EF
AD  - Setor de Virologia, Departamento de Medicina Veterinaria Preventiva, Universidade 
      Federal de Santa Maria, Av. Roraima, 1000, predio 20, sala 4200, Santa Maria, RS, 
      CEP 97105-900, Brazil. Electronic address: eduardofurtadoflores@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150714
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Brain/virology
MH  - Central Nervous System Infections/pathology/virology
MH  - Dexamethasone/administration & dosage
MH  - *Disease Models, Animal
MH  - Herpesviridae Infections/*pathology/virology
MH  - Herpesvirus 1, Equid/*isolation & purification
MH  - Lung/virology
MH  - Olfactory Bulb/virology
MH  - Rabbits
MH  - Respiratory Tract Infections/pathology/virology
MH  - Survival Analysis
MH  - Trigeminal Ganglion/virology
MH  - Virus Activation/drug effects
MH  - Virus Shedding
OTO - NOTNLM
OT  - Acute disease
OT  - Animal model
OT  - EHV-1
OT  - Latency
OT  - New Zealand rabbits
EDAT- 2015/07/19 06:00
MHDA- 2016/05/26 06:00
CRDT- 2015/07/19 06:00
PHST- 2015/03/15 00:00 [received]
PHST- 2015/05/28 00:00 [revised]
PHST- 2015/07/11 00:00 [accepted]
PHST- 2015/07/19 06:00 [entrez]
PHST- 2015/07/19 06:00 [pubmed]
PHST- 2016/05/26 06:00 [medline]
AID - S0882-4010(15)00107-2 [pii]
AID - 10.1016/j.micpath.2015.07.007 [doi]
PST - ppublish
SO  - Microb Pathog. 2015 Oct;87:45-50. doi: 10.1016/j.micpath.2015.07.007. Epub 2015 
      Jul 14.