PMID- 26187665
OWN - NLM
STAT- MEDLINE
DCOM- 20151110
LR  - 20150811
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 464
IP  - 3
DP  - 2015 Aug 28
TI  - Long non-coding RNA HOTAIR promotes HLA-G expression via inhibiting miR-152 in 
      gastric cancer cells.
PG  - 807-13
LID - S0006-291X(15)30271-0 [pii]
LID - 10.1016/j.bbrc.2015.07.040 [doi]
AB  - Recent studies have shown that the long non-coding RNA HOTAIR plays critical 
      roles in tumor biology, including cancer progression and metastasis. However, the 
      potential biological role HOTAIR in tumor escape remains undefined. Here, HOTAIR 
      expression was measured in sixty paired gastric cancer (GC) tissue samples by 
      real-time PCR, and then subjected to correlation analysis with human leukocyte 
      antigen (HLA)-G levels which show close links with tumor escape mechanisms. 
      Significant HOTAIR overexpression was observed in GC tissues, as well as strong 
      positive correlations with HLA-G levels in both tissue and peripheral blood 
      samples, detected by real-time PCR and ELISA assays respectively. Further gain- 
      and loss-of-function studies indicated that HLA-G could be upregulated HOTAIR at 
      both mRNA and secretion levels in vitro. On the other hand, bioinformatics 
      analysis indicated the interaction between HOTAIR and miR-152, which shows 
      potential regulation on HLA-G. And, altered miR-152 expression in GC tissues was 
      also identified, and showed negative correlation with HOTAIR expression. 
      Moreover, the negative regulation of miR-152 on HLA-G was verified in GC cells, 
      while miR-152 induced decrease of HLA-G 3'UTR activity could be attenuated by 
      HOTAIR co-overexpression with the assistant of mutation studies. Therefore, it 
      was concluded that HOTAIR overexpression might also get involved in tumor escape 
      mechanisms, involving HLA-G upregulation via inhibiting miR-152. Furthermore, 
      this study recommended the potential application of HOTAIR in GC immunotherapy 
      for better prognosis and improved survival.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Song, Bingtan
AU  - Song B
AD  - Department of General Surgery, Qilu Hospital, Shandong University, Jinan, 
      Shandong, 250012, China; Department of General Surgery, Affiliated Hospital of 
      Binzhou Medical University, Binzhou, Shandong, 256603, China.
FAU - Guan, Zhongzheng
AU  - Guan Z
AD  - Department of General Surgery, Qilu Hospital, Shandong University, Jinan, 
      Shandong, 250012, China; Department of General Surgery, Liaocheng Third People's 
      Hospital, Liaocheng, Shandong, 252000, China.
FAU - Liu, Fengjun
AU  - Liu F
AD  - Department of General Surgery, Qilu Hospital, Shandong University, Jinan, 
      Shandong, 250012, China. Electronic address: fengjliuj@163.com.
FAU - Sun, Dong
AU  - Sun D
AD  - Department of General Surgery, Qilu Hospital, Shandong University, Jinan, 
      Shandong, 250012, China.
FAU - Wang, Kexin
AU  - Wang K
AD  - Department of General Surgery, Qilu Hospital, Shandong University, Jinan, 
      Shandong, 250012, China.
FAU - Qu, Hui
AU  - Qu H
AD  - Department of General Surgery, Qilu Hospital, Shandong University, Jinan, 
      Shandong, 250012, China.
LA  - eng
PT  - Journal Article
DEP - 20150714
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (3' Untranslated Regions)
RN  - 0 (HLA-G Antigens)
RN  - 0 (HOTAIR long untranslated RNA, human)
RN  - 0 (MIRN152 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Case-Control Studies
MH  - Cell Line, Tumor
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - HLA-G Antigens/blood/*genetics
MH  - Humans
MH  - Male
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - RNA, Long Noncoding/*genetics
MH  - Stomach Neoplasms/*genetics/pathology
OTO - NOTNLM
OT  - Gastric cancer
OT  - HLA-G
OT  - HOTAIR
OT  - Tumor escape
OT  - miR-152
EDAT- 2015/07/19 06:00
MHDA- 2015/11/11 06:00
CRDT- 2015/07/19 06:00
PHST- 2015/07/06 00:00 [received]
PHST- 2015/07/07 00:00 [accepted]
PHST- 2015/07/19 06:00 [entrez]
PHST- 2015/07/19 06:00 [pubmed]
PHST- 2015/11/11 06:00 [medline]
AID - S0006-291X(15)30271-0 [pii]
AID - 10.1016/j.bbrc.2015.07.040 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2015 Aug 28;464(3):807-13. doi: 
      10.1016/j.bbrc.2015.07.040. Epub 2015 Jul 14.