PMID- 26187925
OWN - NLM
STAT- MEDLINE
DCOM- 20160808
LR  - 20151217
IS  - 1945-239X (Electronic)
IS  - 0021-9665 (Linking)
VI  - 54
IP  - 1
DP  - 2016 Jan
TI  - Simultaneous Determination of Five Alkaloid Compounds in a Drug Based on a 
      Hydrophilic Monolithic Column by Capillary Electrochromatography.
PG  - 88-95
LID - 10.1093/chromsci/bmv096 [doi]
AB  - A novel capillary electrochromatography (CEC) method was developed by using a 
      hydrophilic monolithic column for the simultaneous determination of five 
      alkaloids in a drug. The monolithic stationary phase was first prepared via in 
      situ polymerization of acrylamide (AM), glycidyl methacrylate (GMA), 
      N,N'-methylenebisacrylamide (MBA) and 2-acrylamido-2-methyl-1-propane-sulfonic 
      acid (AMPS) in a ternary porogen solvent system consisting of cyclohexanol, 
      dodecanol and toluene. The obtained monolithic stationary phase was subsequently 
      modified by 0.1 mol/L ammonia water for opening epoxide groups of GMA. The 
      separation performance and efficiency of the resulting monolithic column were 
      investigated by the use of five compounds (thiourea, aniline, naphthylamine, 
      diphenylamine and dimethyl acetamide) by CEC. The optimized monolithic column has 
      obtained high column efficiencies with 74,000-121,000 theoretical plates/m. 
      Finally, the prepared monolithic column was used to separate and determine five 
      alkaloids (piperine, nuciferine, kukoline, berberine and tetrandrine) using CEC. 
      Under the conditions of acetonitrile/10 mM phosphate buffer solution (65/35, v/v, 
      pH 8.5) and 15 kV applied voltage, the baseline separation of five alkaloids was 
      achieved. The proposed method has been successfully applied to the determination 
      of berberine in a tablet sample. The percentage of recovery of spiked tablet 
      samples ranged from 93.4 to 108.0% with relative standard deviations (RSDs) 
      <9.20%.
CI  - (c) The Author 2015. Published by Oxford University Press. All rights reserved. For 
      Permissions, please email: journals.permissions@oup.com.
FAU - Chen, Zongbao
AU  - Chen Z
AD  - Key Laboratory of Applied Organic Chemistry, College of Jiangxi Province, 
      Department of Chemistry, Shangrao Normal University, Shangrao, Jiangxi 334001, PR 
      China Ministry of Education Key Laboratory of Analysis and Detection for Food 
      Safety, Fuzhou University, Fuzhou, Fujian 335002, PR China 
      chenzongbao19780920@126.com.
FAU - Ye, Qing
AU  - Ye Q
AD  - Key Laboratory of Applied Organic Chemistry, College of Jiangxi Province, 
      Department of Chemistry, Shangrao Normal University, Shangrao, Jiangxi 334001, PR 
      China.
FAU - Liu, Linghai
AU  - Liu L
AD  - Key Laboratory of Applied Organic Chemistry, College of Jiangxi Province, 
      Department of Chemistry, Shangrao Normal University, Shangrao, Jiangxi 334001, PR 
      China.
FAU - Dong, Hongxia
AU  - Dong H
AD  - Key Laboratory of Applied Organic Chemistry, College of Jiangxi Province, 
      Department of Chemistry, Shangrao Normal University, Shangrao, Jiangxi 334001, PR 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150716
PL  - United States
TA  - J Chromatogr Sci
JT  - Journal of chromatographic science
JID - 0173225
RN  - 0 (Alkaloids)
RN  - 0 (Buffers)
SB  - IM
MH  - Alkaloids/*analysis
MH  - Buffers
MH  - Capillary Electrochromatography/*methods
MH  - Hydrogen-Ion Concentration
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Microscopy, Electron, Scanning
MH  - Reproducibility of Results
EDAT- 2015/07/19 06:00
MHDA- 2016/08/09 06:00
CRDT- 2015/07/19 06:00
PHST- 2015/04/09 00:00 [received]
PHST- 2015/07/19 06:00 [entrez]
PHST- 2015/07/19 06:00 [pubmed]
PHST- 2016/08/09 06:00 [medline]
AID - bmv096 [pii]
AID - 10.1093/chromsci/bmv096 [doi]
PST - ppublish
SO  - J Chromatogr Sci. 2016 Jan;54(1):88-95. doi: 10.1093/chromsci/bmv096. Epub 2015 
      Jul 16.