PMID- 26188295 OWN - NLM STAT- MEDLINE DCOM- 20160524 LR - 20181202 IS - 1879-0003 (Electronic) IS - 0141-8130 (Linking) VI - 80 DP - 2015 Sep TI - PEG-based ultrasound-assisted enzymatic extraction of polysaccharides from Ginkgo biloba leaves. PG - 644-50 LID - S0141-8130(15)00484-5 [pii] LID - 10.1016/j.ijbiomac.2015.07.023 [doi] AB - In this study, one kind of environmentally friendly solvents named polyethylene glycol (PEG) was developed for the ultrasound-assisted enzymatic extraction (UAEE) of polysaccharides from Gingko biloba leaves (GBLP). Box-Behnken design (BBD) was used to optimize the UAEE conditions of GBLP. Results showed that the optimal extraction conditions were: a pH of 4.34, an extraction temperature of 51.88 degrees C and an extraction time of 37.13 min. Under these optimal extraction conditions, the GBLP yield was 7.29+/-0.21%, which was well in agreement with the value predicted by the mathematical model. CI - Copyright (c) 2015 Elsevier B.V. All rights reserved. FAU - Zhang, Lijin AU - Zhang L AD - Department of Food Science and Nutrition, School of Hotel Management, University of Jinan, No. 13, Shungeng Road, Jinan, Shandong 250002, PR China. FAU - Guo, Shanshan AU - Guo S AD - Department of Food Science and Nutrition, School of Hotel Management, University of Jinan, No. 13, Shungeng Road, Jinan, Shandong 250002, PR China. FAU - Wang, Maoshan AU - Wang M AD - Department of Food Science and Nutrition, School of Hotel Management, University of Jinan, No. 13, Shungeng Road, Jinan, Shandong 250002, PR China. FAU - He, Lei AU - He L AD - Department of Food Science and Nutrition, School of Hotel Management, University of Jinan, No. 13, Shungeng Road, Jinan, Shandong 250002, PR China. Electronic address: helei0508@sina.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150716 PL - Netherlands TA - Int J Biol Macromol JT - International journal of biological macromolecules JID - 7909578 RN - 0 (Plant Extracts) RN - 0 (Polysaccharides) RN - 0 (Solvents) RN - 3WJQ0SDW1A (Polyethylene Glycols) SB - IM MH - Chemical Fractionation/*methods MH - Ginkgo biloba/*chemistry MH - Models, Theoretical MH - Plant Extracts/*chemistry MH - Plant Leaves/*chemistry MH - Polyethylene Glycols/*chemistry MH - Polysaccharides/*chemistry MH - Solvents MH - *Sound MH - Temperature OTO - NOTNLM OT - Box-Behnken design OT - Ginkgo biloba leaves OT - Polyethylene glycol-based ultrasound-assisted enzymatic extraction OT - Polysaccharides OT - Response surface methodology EDAT- 2015/07/19 06:00 MHDA- 2016/05/25 06:00 CRDT- 2015/07/19 06:00 PHST- 2015/04/10 00:00 [received] PHST- 2015/06/24 00:00 [revised] PHST- 2015/07/11 00:00 [accepted] PHST- 2015/07/19 06:00 [entrez] PHST- 2015/07/19 06:00 [pubmed] PHST- 2016/05/25 06:00 [medline] AID - S0141-8130(15)00484-5 [pii] AID - 10.1016/j.ijbiomac.2015.07.023 [doi] PST - ppublish SO - Int J Biol Macromol. 2015 Sep;80:644-50. doi: 10.1016/j.ijbiomac.2015.07.023. Epub 2015 Jul 16.