PMID- 26189024
OWN - NLM
STAT- MEDLINE
DCOM- 20160721
LR  - 20220311
IS  - 1879-0712 (Electronic)
IS  - 0014-2999 (Linking)
VI  - 764
DP  - 2015 Oct 5
TI  - Effect of silodosin, a selective alpha(1A)-adrenoceptor antagonist, on voiding 
      behavior and bladder blood flow in a rat model of bladder outlet obstruction.
PG  - 489-496
LID - S0014-2999(15)30165-5 [pii]
LID - 10.1016/j.ejphar.2015.07.037 [doi]
AB  - This study was performed to investigate the effects of silodosin (selective 
      alpha1A-adrenoceptor antagonist) on bladder blood flow (BBF) and bladder function in 
      a rat model of bladder outlet obstruction (BOO) and to determine the expression 
      of alpha1-adrenoceptor subtype mRNA in human and rat bladder microvessels. BOO was 
      produced by partial ligature of the proximal urethra, which was maintained for 2 
      weeks. The BOO rats received either silodosin at a rate of 0.3mg/kg/day or 
      vehicle subcutaneously via an osmotic pump for 2 weeks after BOO surgery. A 
      metabolic cage study was performed in conscious animals. BBF was measured using a 
      Laser Speckle Blood Flow Imager. Urinary levels of 8-hydroxy-2'-deoxyguanosine 
      (8-OHdG) and nerve growth factor (NGF) were measured. Immunohistological 
      examinations of nerve distribution and NGF expression in the rat bladder were 
      conducted. The expression of each alpha1-adrenoceptor subtype mRNA in human and rat 
      bladder microvessels was determined by in situ hybridization. Silodosin 
      ameliorated the increase in voiding frequency and decrease in mean voided volume 
      in BOO rats in the metabolic cage study. Silodosin also abrogated the decrease in 
      BBF in BOO rats. The levels of 8-OHdG and NGF in BOO rats were significantly 
      decreased by administration of silodosin. Silodosin prevented the decrease in 
      nerve distribution and increase in NGF expression. Human and rat bladder 
      microvessels showed expression of all alpha1-adrenoceptor subtype mRNAs. The results 
      presented here suggest that silodosin improves voiding behavior in rat models 
      with BOO by inducing recovery of BBF.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Goi, Yoshiaki
AU  - Goi Y
AD  - Pharmacology Research Laboratory, Kissei Pharmaceutical Co. Ltd., Azumino City, 
      Japan. Electronic address: yoshiaki_goi@pharm.kissei.co.jp.
FAU - Tomiyama, Yoshitaka
AU  - Tomiyama Y
AD  - Pharmacology Research Laboratory, Kissei Pharmaceutical Co. Ltd., Azumino City, 
      Japan.
FAU - Yokoyama, Ayaka
AU  - Yokoyama A
AD  - Pharmacology Research Laboratory, Kissei Pharmaceutical Co. Ltd., Azumino City, 
      Japan.
FAU - Tatemichi, Satoshi
AU  - Tatemichi S
AD  - Pharmacology Research Laboratory, Kissei Pharmaceutical Co. Ltd., Azumino City, 
      Japan.
FAU - Maruyama, Kazuyasu
AU  - Maruyama K
AD  - Pharmacology Research Laboratory, Kissei Pharmaceutical Co. Ltd., Azumino City, 
      Japan.
FAU - Kobayashi, Mamoru
AU  - Kobayashi M
AD  - Pharmacology Research Laboratory, Kissei Pharmaceutical Co. Ltd., Azumino City, 
      Japan.
FAU - Yamaguchi, Osamu
AU  - Yamaguchi O
AD  - Division of Bioengineering and LUTD Research, Nihon University School of 
      Engineering, Koriyama City, Japan.
LA  - eng
PT  - Journal Article
DEP - 20150717
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (ADRA1A protein, human)
RN  - 0 (Adra1a protein, rat)
RN  - 0 (Adrenergic alpha-1 Receptor Antagonists)
RN  - 0 (Indoles)
RN  - 0 (Receptors, Adrenergic, alpha-1)
RN  - 0 (Urological Agents)
RN  - 88847-89-6 (8-Hydroxy-2'-Deoxyguanosine)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - CUZ39LUY82 (silodosin)
RN  - G9481N71RO (Deoxyguanosine)
SB  - IM
MH  - 8-Hydroxy-2'-Deoxyguanosine
MH  - Adrenergic alpha-1 Receptor Antagonists/*pharmacology
MH  - Animals
MH  - Blood Flow Velocity
MH  - Deoxyguanosine/analogs & derivatives/urine
MH  - Disease Models, Animal
MH  - Female
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Indoles/*pharmacology
MH  - Laser-Doppler Flowmetry
MH  - Microcirculation/*drug effects
MH  - Nerve Growth Factor/urine
MH  - Rats, Sprague-Dawley
MH  - Receptors, Adrenergic, alpha-1/*drug effects/genetics/metabolism
MH  - Regional Blood Flow
MH  - Time Factors
MH  - Ureteral Obstruction/complications
MH  - Urinary Bladder/blood supply/*drug effects/innervation/metabolism
MH  - Urinary Bladder Neck Obstruction/*drug 
      therapy/etiology/genetics/metabolism/physiopathology
MH  - Urination/*drug effects
MH  - Urodynamics/*drug effects
MH  - Urological Agents/*pharmacology
OTO - NOTNLM
OT  - Bladder blood flow
OT  - Bladder outlet obstruction
OT  - Ischemia
OT  - Nerve growth factor
OT  - Silodosin
OT  - Silodosin (PubChem CID: 5312125)
OT  - alpha(1A)-Adrenoceptor
EDAT- 2015/07/21 06:00
MHDA- 2016/07/22 06:00
CRDT- 2015/07/20 06:00
PHST- 2015/03/24 00:00 [received]
PHST- 2015/06/18 00:00 [revised]
PHST- 2015/07/15 00:00 [accepted]
PHST- 2015/07/20 06:00 [entrez]
PHST- 2015/07/21 06:00 [pubmed]
PHST- 2016/07/22 06:00 [medline]
AID - S0014-2999(15)30165-5 [pii]
AID - 10.1016/j.ejphar.2015.07.037 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 2015 Oct 5;764:489-496. doi: 10.1016/j.ejphar.2015.07.037. Epub 
      2015 Jul 17.