PMID- 26189884
OWN - NLM
STAT- MEDLINE
DCOM- 20160422
LR  - 20151023
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 231
IP  - 2
DP  - 2016 Feb
TI  - D-Aspartate Induces Proliferative Pathways in Spermatogonial GC-1 Cells.
PG  - 490-5
LID - 10.1002/jcp.25095 [doi]
AB  - D-aspartate (D-Asp) is an endogenous amino acid present in vertebrate tissues, 
      with particularly high levels in the testis. In vivo studies indicate that D-Asp 
      indirectly stimulates spermatogenesis through the hypothalamic-pituitary-gonadal 
      axis. Moreover, in vitro studies have demonstrated that D-Asp up-regulates 
      testosterone production in Leydig cells by enhancing expression of the 
      steroidogenic acute regulatory protein. In this study, a cell line derived from 
      immortalized type-B mouse spermatogonia retaining markers of mitotic germ cells 
      (GC-1) was employed to explore more direct involvement of D-Asp in 
      spermatogenesis. Activity and protein expression of markers of cell proliferation 
      were determined at intervals during incubation in D-Asp-containing medium. D-Asp 
      induced phosphorylation of ERK and Akt proteins, stimulated expression of PCNA 
      and Aurora B, and enhanced mRNA synthesis and protein expression of P450 
      aromatase and protein expression of Estrogen Receptor beta (ERbeta). These results are 
      the first demonstration of a direct effect of D-Asp on spermatogonial mitotic 
      activity. Considering that spermatogonia express the NR1 subunit of the 
      N-Methyl-D-Aspartic Acid receptor (NMDAR), we suggest that their response to 
      D-Asp depends on NMDAR-mediated activation of the ERK and Akt pathways and is 
      further enhanced by activation of the P450 aromatase/ERbeta pathway.
CI  - (c) 2015 Wiley Periodicals, Inc.
FAU - Santillo, Alessandra
AU  - Santillo A
AD  - Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, 
      Seconda Universita di Napoli, Caserta, Italy.
FAU - Falvo, Sara
AU  - Falvo S
AD  - Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, 
      Seconda Universita di Napoli, Caserta, Italy.
FAU - Chieffi, Paolo
AU  - Chieffi P
AD  - Dipartimento di Psicologia, Seconda Universita di Napoli, Caserta, Italy.
FAU - Di Fiore, Maria Maddalena
AU  - Di Fiore MM
AD  - Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, 
      Seconda Universita di Napoli, Caserta, Italy.
FAU - Senese, Rosalba
AU  - Senese R
AD  - Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, 
      Seconda Universita di Napoli, Caserta, Italy.
FAU - Chieffi Baccari, Gabriella
AU  - Chieffi Baccari G
AD  - Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, 
      Seconda Universita di Napoli, Caserta, Italy.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 0 (RNA, Messenger)
RN  - 4SR0Q8YD1X (D-Aspartic Acid)
RN  - EC 1.14.14.1 (Aromatase)
RN  - EC 2.7.11.1 (Aurkb protein, mouse)
RN  - EC 2.7.11.1 (Aurora Kinase B)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Animals
MH  - Aromatase/genetics/metabolism
MH  - Aurora Kinase B/metabolism
MH  - Cell Line
MH  - Cell Proliferation/drug effects/genetics/physiology
MH  - D-Aspartic Acid/*metabolism/*pharmacology
MH  - MAP Kinase Signaling System
MH  - Male
MH  - Mice
MH  - Models, Biological
MH  - Proliferating Cell Nuclear Antigen/metabolism
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Signal Transduction/drug effects
MH  - Spermatogenesis/*drug effects/genetics/*physiology
MH  - Spermatogonia/*cytology/*drug effects/metabolism
EDAT- 2015/07/21 06:00
MHDA- 2016/04/23 06:00
CRDT- 2015/07/21 06:00
PHST- 2015/06/03 00:00 [received]
PHST- 2015/07/17 00:00 [accepted]
PHST- 2015/07/21 06:00 [entrez]
PHST- 2015/07/21 06:00 [pubmed]
PHST- 2016/04/23 06:00 [medline]
AID - 10.1002/jcp.25095 [doi]
PST - ppublish
SO  - J Cell Physiol. 2016 Feb;231(2):490-5. doi: 10.1002/jcp.25095.