PMID- 26193851
OWN - NLM
STAT- MEDLINE
DCOM- 20151120
LR  - 20150904
IS  - 1432-0584 (Electronic)
IS  - 0939-5555 (Linking)
VI  - 94
IP  - 10
DP  - 2015 Oct
TI  - Early detection of cytomegalovirus-specific cytotoxic T lymphocytes against 
      cytomegalovirus antigenemia in human leukocyte antigen haploidentical 
      hematopoietic stem cell transplantation.
PG  - 1707-15
LID - 10.1007/s00277-015-2446-4 [doi]
AB  - Human leukocyte antigen (HLA)-haploidentical stem cell transplantation 
      (haplo-SCT) is associated with a high incidence of cytomegalovirus (CMV) 
      infection, probably originating from the delayed reconstitution of CMV-specific T 
      cell immunity. There have been few reports on the presence of CMV-specific 
      cytotoxic T lymphocytes (CMV-CTLs) after haplo-SCT. We have studied CMV-specific 
      immune reconstitution by measuring the absolute number of CMV-CTLs using a flow 
      cytometry method with HLA-A2-restricted NLVPMVATV peptide dextramers. We examined 
      the association between reconstitution patterns of CMV-CTLs and the duration of 
      CMV antigenemia in 15 patients who underwent first allogeneic SCT from 
      HLA-haploidentical-related donors with HLA-A2. In seven and eight patients, CMV 
      antigenemia consecutively resolved for more than 4 weeks (the CMV antigenemia 
      'resolved' group) and intermittently persisted (the CMV antigenemia 'persistent' 
      group) during a 100-day observation period, respectively. The group of the seven 
      patients, in whom levels of CMV antigenemia were reduced to zero, had a 
      significantly lower maximum level of CMV antigenemia than the CMV antigenemia 
      persistent group. In contrast, the CMV antigenemia persistent group had a 
      significantly higher maximum level of CMV-CTLs, but the levels took longer to 
      peak. Despite no difference in general lymphocyte recovery between the two 
      groups, the CMV antigenemia resolved group had significantly higher median 
      CMV-CTL counts than the CMV antigenemia persistent group at 6 weeks after onset 
      of CMV infection. Flow cytometry analysis of CMV-CTLs is a convenient method of 
      monitoring reconstitution of CMV-specific lymphocyte immunity following 
      haplo-SCT.
FAU - Kato, Ruri
AU  - Kato R
AD  - Division of Hematology, Department of Internal Medicine, Hyogo College of 
      Medicine, 1-1 Mukogawa-cho, Nishinomiya City, Hyogo, 663-8501, Japan, 
      kato.ruri@k.nissay-hp.or.jp.
FAU - Tamaki, Hiroya
AU  - Tamaki H
FAU - Ikegame, Kazuhiro
AU  - Ikegame K
FAU - Yoshihara, Satoshi
AU  - Yoshihara S
FAU - Kaida, Katsuji
AU  - Kaida K
FAU - Taniguchi, Kyoko
AU  - Taniguchi K
FAU - Inoue, Takayuki
AU  - Inoue T
FAU - Ishii, Shinichi
AU  - Ishii S
FAU - Nakata, Jun
AU  - Nakata J
FAU - Fujioka, Tatsuya
AU  - Fujioka T
FAU - Eguchi, Ryoji
AU  - Eguchi R
FAU - Soma, Toshihiro
AU  - Soma T
FAU - Okada, Masaya
AU  - Okada M
FAU - Ogawa, Hiroyasu
AU  - Ogawa H
LA  - eng
PT  - Journal Article
DEP - 20150722
PL  - Germany
TA  - Ann Hematol
JT  - Annals of hematology
JID - 9107334
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Adult
MH  - Cytomegalovirus/*immunology/isolation & purification
MH  - Cytomegalovirus Infections/*diagnosis/*immunology/mortality
MH  - Female
MH  - Follow-Up Studies
MH  - HLA Antigens/*immunology
MH  - Haplotypes
MH  - Hematopoietic Stem Cell Transplantation/*adverse effects
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Survival Rate/trends
MH  - T-Lymphocytes, Cytotoxic/*immunology/virology
MH  - Transplantation, Homologous
EDAT- 2015/07/22 06:00
MHDA- 2015/12/15 06:00
CRDT- 2015/07/22 06:00
PHST- 2014/10/15 00:00 [received]
PHST- 2015/07/07 00:00 [accepted]
PHST- 2015/07/22 06:00 [entrez]
PHST- 2015/07/22 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - 10.1007/s00277-015-2446-4 [doi]
PST - ppublish
SO  - Ann Hematol. 2015 Oct;94(10):1707-15. doi: 10.1007/s00277-015-2446-4. Epub 2015 
      Jul 22.