PMID- 26194818
OWN - NLM
STAT- MEDLINE
DCOM- 20161028
LR  - 20161230
IS  - 1559-0720 (Electronic)
IS  - 0163-4984 (Linking)
VI  - 170
IP  - 1
DP  - 2016 Mar
TI  - Increased Oxidative DNA Damage in Placenta Contributes to Cadmium-Induced 
      Preeclamptic Conditions in Rat.
PG  - 119-27
LID - 10.1007/s12011-015-0438-9 [doi]
AB  - To explore the possible mechanisms of cadmium (Cd)-induced preeclamptic 
      conditions in rats. In the present study, we introduced the in vivo model of 
      preeclampsia by giving intraperitoneal injections of cadmium chloride (CdCl2) to 
      pregnant rats from gestational day (GD) 4 to 19. Maternal body weights were 
      recorded on GD 0, 14, and 20, while their systolic blood pressures (SBPs) 
      monitored on GD 3, 11, and 18. On GD 20, rats were sacrificed and the specimens 
      were collected. The morphological changes of placenta and kidney tissues of 
      pregnant rats were examined by hematoxylin and eosin staining assay. Blood Cd 
      level was detected by inductively coupled plasma mass spectrometry. Total 
      antioxidant capacity (TAC) was evaluated using FRAP method and total nitrite 
      (NOx) was detected with Griess reagent. Antioxidative factors and DNA 
      damage/repair biomarkers were measured by real-time qPCR, western blot or 
      immunohistochemistry study. The current results showed that CdCl2-treated 
      pregnant rats developed preeclampsia (PE)-like manifestations, such as 
      hypertension, albuminuria, with decreased TAC and increased blood Cd level, and 
      pro-oxidative/antioxidative or DNA damage/repair biomarkers. Our study 
      demonstrated that increased oxidative DNA damage in placenta could contribute to 
      Cd-induced preeclamptic conditions in rat.
FAU - Zhang, Xiaojie
AU  - Zhang X
AD  - Department of Gynecology and Obstetrics, The First Affiliated Hospital of Wenzhou 
      Medical University, Wenzhou, 325000, China.
FAU - Xu, Zhangye
AU  - Xu Z
AD  - Department of Gynecology and Obstetrics, The Second Affiliated Hospital of 
      Wenzhou Medical University, Wenzhou, 325000, China.
FAU - Lin, Feng
AU  - Lin F
AD  - Department of Gynecology and Obstetrics, The First Affiliated Hospital of Wenzhou 
      Medical University, Wenzhou, 325000, China.
FAU - Wang, Fan
AU  - Wang F
AD  - Department of Gynecology and Obstetrics, The Second Affiliated Hospital of 
      Wenzhou Medical University, Wenzhou, 325000, China.
FAU - Ye, Duyun
AU  - Ye D
AD  - Department of Pathophysiology, Tongji Medical College, Huazhong University of 
      Science and Technology, Wuhan, 430030, China.
FAU - Huang, Yinping
AU  - Huang Y
AD  - Department of Gynecology and Obstetrics, The First Affiliated Hospital of Wenzhou 
      Medical University, Wenzhou, 325000, China. yphuangp@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150722
PL  - United States
TA  - Biol Trace Elem Res
JT  - Biological trace element research
JID - 7911509
RN  - J6K4F9V3BA (Cadmium Chloride)
SB  - IM
MH  - Animals
MH  - Cadmium Chloride/*toxicity
MH  - *DNA Damage
MH  - Female
MH  - *Oxidative Stress
MH  - Placenta/*metabolism
MH  - Pre-Eclampsia/*chemically induced
MH  - Pregnancy
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - Cadmium chloride
OT  - DNA repair
OT  - Hypertension
OT  - Oxidative stress
OT  - Preeclampsia
EDAT- 2015/07/22 06:00
MHDA- 2016/11/01 06:00
CRDT- 2015/07/22 06:00
PHST- 2015/05/21 00:00 [received]
PHST- 2015/07/05 00:00 [accepted]
PHST- 2015/07/22 06:00 [entrez]
PHST- 2015/07/22 06:00 [pubmed]
PHST- 2016/11/01 06:00 [medline]
AID - 10.1007/s12011-015-0438-9 [pii]
AID - 10.1007/s12011-015-0438-9 [doi]
PST - ppublish
SO  - Biol Trace Elem Res. 2016 Mar;170(1):119-27. doi: 10.1007/s12011-015-0438-9. Epub 
      2015 Jul 22.