PMID- 26195547
OWN - NLM
STAT- MEDLINE
DCOM- 20160218
LR  - 20181113
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Print)
IS  - 0019-9567 (Linking)
VI  - 83
IP  - 10
DP  - 2015 Oct
TI  - Pseudomonas aeruginosa Quorum Sensing Molecule 
      N-(3-Oxododecanoyl)-L-Homoserine-Lactone Induces HLA-G Expression in Human Immune 
      Cells.
PG  - 3918-25
LID - 10.1128/IAI.00803-15 [doi]
AB  - HLA-G is a nonclassical class I human leukocyte antigen (HLA) involved in 
      mechanisms of immune tolerance. The objective of this study was to determine 
      whether N-(3-oxododecanoyl)-l-homoserine lactone (3O-C12-HSL), a quorum sensing 
      molecule produced by Pseudomonas aeruginosa, could modify HLA-G expression to 
      control the host immune response. We evaluated the ability of 3O-C12-HSL to 
      induce HLA-G expression in primary immune cells, monocytes (U937 and THP1), and 
      T-cell lines (Jurkat) in vitro and analyzed the cellular pathway responsible for 
      HLA-G expression. We studied the HLA-G promoter with a luciferase assay and 
      interleukin-10 (IL-10) and p38/CREB signaling with enzyme-linked immunosorbent 
      assay and immunofluorescence, respectively. We observed that 3O-C12-HSL is able 
      to induce HLA-G expression in human monocytes and T cells. We showed that the 
      induction of HLA-G by 3O-C12-HSL is p38/CREB and IL-10 dependent. 3O-C12-HSL 
      treatment is able to arrest only the U937 cell cycle, possibly due to the 
      peculiar expression of the ILT2 receptor in the U937 cell line. Our observations 
      suggest HLA-G as a mechanism to create a protected niche for the bacterial 
      reservoir, similar to the role of HLA-G molecules during viral infections.
CI  - Copyright (c) 2015, American Society for Microbiology. All Rights Reserved.
FAU - Bortolotti, Daria
AU  - Bortolotti D
AD  - Department of Medical Sciences, Section of Microbiology and Medical Genetics, 
      University of Ferrara, Ferrara, Italy.
FAU - LeMaoult, Joel
AU  - LeMaoult J
AD  - CEA, Institute of Emerging Diseases and Innovative Therapies (iMETI), Research 
      Division in Hematology and Immunology (SRHI), Saint-Louis Hospital, Paris, 
      France.
FAU - Trapella, Claudio
AU  - Trapella C
AD  - Department of Chemical and Pharmaceutical Sciences, University of Ferrara, 
      Ferrara, Italy.
FAU - Di Luca, Dario
AU  - Di Luca D
AD  - Department of Medical Sciences, Section of Microbiology and Medical Genetics, 
      University of Ferrara, Ferrara, Italy.
FAU - Carosella, Edgardo D
AU  - Carosella ED
AD  - CEA, Institute of Emerging Diseases and Innovative Therapies (iMETI), Research 
      Division in Hematology and Immunology (SRHI), Saint-Louis Hospital, Paris, 
      France.
FAU - Rizzo, Roberta
AU  - Rizzo R
AD  - Department of Medical Sciences, Section of Microbiology and Medical Genetics, 
      University of Ferrara, Ferrara, Italy rbr@unife.it.
LA  - eng
PT  - Journal Article
DEP - 20150720
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (HLA-G Antigens)
RN  - 0 (IL10 protein, human)
RN  - 0 (N-(3-oxododecanoyl)homoserine lactone)
RN  - 130068-27-8 (Interleukin-10)
RN  - 6KA95X0IVO (Homoserine)
RN  - OL659KIY4X (4-Butyrolactone)
SB  - IM
EIN - Infect Immun. 2017 Nov 17;85(12 ):. PMID: 29150484
MH  - 4-Butyrolactone/*analogs & derivatives/immunology/metabolism
MH  - HLA-G Antigens/*genetics/immunology
MH  - Homoserine/*analogs & derivatives/immunology/metabolism
MH  - Humans
MH  - Interleukin-10/genetics/immunology
MH  - Monocytes/immunology
MH  - Pseudomonas Infections/genetics/*immunology/microbiology
MH  - Pseudomonas aeruginosa/genetics/immunology/*physiology
MH  - *Quorum Sensing
MH  - T-Lymphocytes/immunology
MH  - U937 Cells
MH  - *Up-Regulation
PMC - PMC4567644
EDAT- 2015/07/22 06:00
MHDA- 2016/02/19 06:00
PMCR- 2016/04/01
CRDT- 2015/07/22 06:00
PHST- 2015/06/18 00:00 [received]
PHST- 2015/07/14 00:00 [accepted]
PHST- 2015/07/22 06:00 [entrez]
PHST- 2015/07/22 06:00 [pubmed]
PHST- 2016/02/19 06:00 [medline]
PHST- 2016/04/01 00:00 [pmc-release]
AID - IAI.00803-15 [pii]
AID - 00803-15 [pii]
AID - 10.1128/IAI.00803-15 [doi]
PST - ppublish
SO  - Infect Immun. 2015 Oct;83(10):3918-25. doi: 10.1128/IAI.00803-15. Epub 2015 Jul 
      20.