PMID- 26200228
OWN - NLM
STAT- MEDLINE
DCOM- 20170417
LR  - 20181113
IS  - 1414-431X (Electronic)
IS  - 0100-879X (Print)
IS  - 0100-879X (Linking)
VI  - 48
IP  - 9
DP  - 2015 Sep
TI  - Antibacterial and anti-inflammatory activities of an extract, fractions, and 
      compounds isolated from Gochnatia pulchra aerial parts.
PG  - 822-30
LID - S0100-879X2015005054410 [pii]
LID - 10.1590/1414-431X20154410 [doi]
AB  - This paper reports on the in vitro antibacterial and in vivo anti-inflammatory 
      properties of a hydroethanolic extract of the aerial parts of Gochnatia pulchra 
      (HEGP). It also describes the antibacterial activity of HEGP fractions and of the 
      isolated compounds genkwanin, scutellarin, apigenin, and 3,5-O-dicaffeoylquinic 
      acid, as evaluated by a broth microdilution method. While HEGP and its fractions 
      did not provide promising results, the isolated compounds exhibited pronounced 
      antibacterial activity. The most sensitive microorganism was Streptococcus 
      pyogenes, with minimum inhibitory concentration (MIC) values of 100, 50 and 25 
      microg/mL for genkwanin and the flavonoids apigenin and scutellarin, respectively. 
      Genkwanin produced an MIC value of 25 microg/mL against Enterococcus faecalis. A paw 
      edema model in rats and a pleurisy inflammation model in mice aided investigation 
      of the anti-inflammatory effects of HEGP. This study also evaluated the ability 
      of HEGP to modulate carrageenan-induced interleukin-1 beta (IL-1beta), tumor 
      necrosis factor alpha (TNF-alpha), and monocyte chemoattractant protein-1 (MCP-1) 
      production. Orally administered HEGP (250 and 500 mg/kg) inhibited 
      carrageenan-induced paw edema. Regarding carrageenan-induced pleurisy, HEGP at 
      50, 100, and 250 mg/kg diminished leukocyte migration by 71.43%, 69.24%, and 
      73.34% (P<0.05), respectively. HEGP suppressed IL-1beta and MCP-1 production by 55% 
      and 50% at 50 mg/kg (P<0.05) and 60% and 25% at 100 mg/kg (P<0.05), respectively. 
      HEGP abated TNF-alpha production by macrophages by 6.6%, 33.3%, and 53.3% at 100, 
      250, and 500 mg/kg (P<0.05), respectively. HEGP probably exerts anti-inflammatory 
      effects by inhibiting production of the pro-inflammatory cytokines TNF-alpha, IL-1beta, 
      and MCP-1.
FAU - Lucarini, R
AU  - Lucarini R
AD  - Nucleo de Pesquisas em Ciencias Exatas e Tecnologicas, Universidade de Franca, 
      Franca, SP, BR.
FAU - Tozatti, M G
AU  - Tozatti MG
AD  - Nucleo de Pesquisas em Ciencias Exatas e Tecnologicas, Universidade de Franca, 
      Franca, SP, BR.
FAU - Silva, M L A
AU  - Silva ML
AD  - Nucleo de Pesquisas em Ciencias Exatas e Tecnologicas, Universidade de Franca, 
      Franca, SP, BR.
FAU - Gimenez, V M M
AU  - Gimenez VM
AD  - Centro Universitario Claretiano, SP, BR.
FAU - Pauletti, P M
AU  - Pauletti PM
AD  - Nucleo de Pesquisas em Ciencias Exatas e Tecnologicas, Universidade de Franca, 
      Franca, SP, BR.
FAU - Groppo, M
AU  - Groppo M
AD  - Faculdade de Filosofia, Ciencias e Letras de Ribeirao Preto, Universidade de Sao 
      Paulo, Ribeirao Preto, SP, BR.
FAU - Turatti, I C C
AU  - Turatti IC
AD  - Departamento de Fisica e Quimica, Faculdade de Ciencias Farmaceuticas de Ribeirao 
      Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, BR.
FAU - Cunha, W R
AU  - Cunha WR
AD  - Nucleo de Pesquisas em Ciencias Exatas e Tecnologicas, Universidade de Franca, 
      Franca, SP, BR.
FAU - Martins, C H G
AU  - Martins CH
AD  - Nucleo de Pesquisas em Ciencias Exatas e Tecnologicas, Universidade de Franca, 
      Franca, SP, BR.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150721
PL  - Brazil
TA  - Braz J Med Biol Res
JT  - Brazilian journal of medical and biological research = Revista brasileira de 
      pesquisas medicas e biologicas
JID - 8112917
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Asteraceae/*chemistry/classification
MH  - Disease Models, Animal
MH  - Edema/*drug therapy
MH  - Gas Chromatography-Mass Spectrometry
MH  - Gram-Negative Bacteria/drug effects
MH  - Gram-Positive Bacteria/drug effects
MH  - Inflammation/*drug therapy
MH  - Male
MH  - Microbial Sensitivity Tests
MH  - Plant Extracts/*pharmacology
MH  - Rats
MH  - Rats, Wistar
PMC - PMC4568810
EDAT- 2015/07/23 06:00
MHDA- 2017/04/18 06:00
PMCR- 2015/07/21
CRDT- 2015/07/23 06:00
PHST- 2014/10/23 00:00 [received]
PHST- 2015/04/23 00:00 [accepted]
PHST- 2015/07/23 06:00 [entrez]
PHST- 2015/07/23 06:00 [pubmed]
PHST- 2017/04/18 06:00 [medline]
PHST- 2015/07/21 00:00 [pmc-release]
AID - S0100-879X2015005054410 [pii]
AID - 10.1590/1414-431X20154410 [doi]
PST - ppublish
SO  - Braz J Med Biol Res. 2015 Sep;48(9):822-30. doi: 10.1590/1414-431X20154410. Epub 
      2015 Jul 21.