PMID- 26200952
OWN - NLM
STAT- MEDLINE
DCOM- 20180806
LR  - 20180806
IS  - 1756-185X (Electronic)
IS  - 1756-1841 (Linking)
VI  - 20
IP  - 12
DP  - 2017 Dec
TI  - Do major histocompatibility complex tag single nucleotide polymorphisms 
      accurately identify HLA-B27 in the Turkish population?
PG  - 2035-2039
LID - 10.1111/1756-185X.12719 [doi]
AB  - OBJECTIVE: To evaluate the performance of human leukocyte antigen (HLA)-B27 tag 
      single nucleotide polymorphisms (SNPs) by polymerase chain reaction - restriction 
      fragment length polymorphism (PCR-RFLP). METHODS: We genotyped three SNPs 
      (rs116488202, rs13202464 and rs4349859). The primers were designed using Primer3 
      algorithm via primer-BLAST interface. PCR products were digested by using NlaIII, 
      BmrI and TaqalphaI enzymes. Quality control was performed by DNA sequence analysis. 
      RESULTS: In total, 207 patients with ankylosing spondylitis and 32 healthy 
      controls were included in the study. The sensitivity and specificity of SNPs 
      rs116488202 and rs4349859 in identifying HLA-B27 were identical and adequate at 
      0.946 and 1.000, respectively. On the other hand, the sensitivity and specificity 
      for rs13202464 was 0.878 and 0.934, respectively. The presence of another SNP 
      (rs141774149) in close proximity to rs116488202 complicated the analysis for RFLP 
      and required that we sequence all the T allele carrying samples. CONCLUSION: The 
      SNPs rs116488202 and rs4349859 may have a place in the identification of HLA-B27 
      in the Turkish population; however, methods other than PCR-RFLP should be 
      considered.
CI  - (c) 2015 Asia Pacific League of Associations for Rheumatology and Wiley Publishing 
      Asia Pty Ltd.
FAU - Akar, Servet
AU  - Akar S
AD  - Division of Rheumatology, Department of Internal Medicine, Izmir Katip Celebi 
      University School of Medicine, Izmir, Turkey.
FAU - Igci, Yusuf Z
AU  - Igci YZ
AD  - Department of Medical Biology, School of Medicine, Gaziantep University, 
      Gaziantep, Turkey.
FAU - Sari, Ismail
AU  - Sari I
AD  - Department of Internal Medicine, Division of Rheumatology, Dokuz Eylul University 
      School of Medicine, Izmir, Turkey.
FAU - Pala, Elif
AU  - Pala E
AD  - Department of Medical Biology, School of Medicine, Gaziantep University, 
      Gaziantep, Turkey.
FAU - Geyik, Esra
AU  - Geyik E
AD  - Department of Medical Biology, School of Medicine, Gaziantep University, 
      Gaziantep, Turkey.
FAU - Tas, Mehmet N
AU  - Tas MN
AD  - Department of Internal Medicine, Division of Rheumatology, Dokuz Eylul University 
      School of Medicine, Izmir, Turkey.
FAU - Solmaz, Dilek
AU  - Solmaz D
AD  - Department of Internal Medicine, Division of Rheumatology, Namik Kemal University 
      School of Medicine, Tekirdag, Turkey.
FAU - Cetin, Pinar
AU  - Cetin P
AD  - Department of Internal Medicine, Division of Rheumatology, Dokuz Eylul University 
      School of Medicine, Izmir, Turkey.
FAU - Akkoc, Nurullah
AU  - Akkoc N
AD  - Department of Internal Medicine, Division of Rheumatology, Dokuz Eylul University 
      School of Medicine, Izmir, Turkey.
LA  - eng
PT  - Journal Article
DEP - 20150722
PL  - England
TA  - Int J Rheum Dis
JT  - International journal of rheumatic diseases
JID - 101474930
RN  - 0 (HLA-B27 Antigen)
SB  - IM
MH  - Adult
MH  - Amplified Fragment Length Polymorphism Analysis
MH  - Case-Control Studies
MH  - Female
MH  - Gene Frequency
MH  - HLA-B27 Antigen/*genetics/immunology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polymorphism, Restriction Fragment Length
MH  - *Polymorphism, Single Nucleotide
MH  - Predictive Value of Tests
MH  - Spondylitis, Ankylosing/diagnosis/*genetics/immunology
MH  - Turkey
OTO - NOTNLM
OT  - HLA-B27 antigen
OT  - ankylosing spondylitis
OT  - polymorphism
OT  - restriction fragment length polymorphism
OT  - single nucleotide polymorphism
EDAT- 2015/07/23 06:00
MHDA- 2018/08/07 06:00
CRDT- 2015/07/23 06:00
PHST- 2015/07/23 06:00 [pubmed]
PHST- 2018/08/07 06:00 [medline]
PHST- 2015/07/23 06:00 [entrez]
AID - 10.1111/1756-185X.12719 [doi]
PST - ppublish
SO  - Int J Rheum Dis. 2017 Dec;20(12):2035-2039. doi: 10.1111/1756-185X.12719. Epub 
      2015 Jul 22.