PMID- 26202884
OWN - NLM
STAT- MEDLINE
DCOM- 20160803
LR  - 20151019
IS  - 1460-9568 (Electronic)
IS  - 0953-816X (Linking)
VI  - 42
IP  - 8
DP  - 2015 Oct
TI  - The N-methyl-D-aspartate receptor's neglected subunit - GluN1 matters under 
      normal and hyperbaric conditions.
PG  - 2577-84
LID - 10.1111/ejn.13022 [doi]
AB  - Professional deep-water divers exposed to hyperbaric pressure (HP) above 1.1 MPa 
      develop high-pressure neurological syndrome, which is associated with central 
      nervous system hyperexcitability. It was previously reported that HP augments 
      N-methyl-D-aspartate receptor (NMDAR) synaptic responses, increases neuronal 
      excitability, and potentially causes irreversible neuronal damage. In addition, 
      we have reported that HP (10.1 MPa) differentially affects ionic currents, 
      measured by the two-electrode voltage-clamp technique, of eight specific NMDAR 
      subtypes generated by the co-expression of GluN1-1a or GluN1-1b with one of the 
      four GluN2(A-D) subunits in Xenopus laevis oocytes. We now report that eight 
      GluN1 splice variants, when co-expressed with GluN2A, mediate different ionic 
      currents at normal and HP (5.1 MPa). These data, in conjunction with our previous 
      results, indicate that both GluN1 and GluN2 subunits play a critical role in 
      determining NMDAR currents under normal and HP conditions. These data, given the 
      differential spatial distribution of the different NMDAR subtypes in the central 
      nervous system, may offer a partial explanation for the mechanism governing the 
      complex signs and symptoms of high-pressure neurological syndrome, and an 
      explanation for the suspected long-term HP health decrement due to repetitive 
      deep dives by professional divers.
CI  - (c) 2015 Federation of European Neuroscience Societies and John Wiley & Sons Ltd.
FAU - Bliznyuk, Alice
AU  - Bliznyuk A
AD  - Department of Physiology and Cell Biology, Faculty of Health Sciences and 
      Zlotowski Center for Neuroscience, Ben Gurion University of the Negev, 
      Beer-Sheva, 84105, Israel.
FAU - Aviner, Ben
AU  - Aviner B
AD  - Department of Physiology and Cell Biology, Faculty of Health Sciences and 
      Zlotowski Center for Neuroscience, Ben Gurion University of the Negev, 
      Beer-Sheva, 84105, Israel.
FAU - Golan, Hava
AU  - Golan H
AD  - Department of Physiology and Cell Biology, Faculty of Health Sciences and 
      Zlotowski Center for Neuroscience, Ben Gurion University of the Negev, 
      Beer-Sheva, 84105, Israel.
FAU - Hollmann, Michael
AU  - Hollmann M
AD  - Department of Biochemistry I, Receptor Biochemistry, Faculty of Chemistry and 
      Biochemistry, Ruhr University Bochum, Bochum, Germany.
FAU - Grossman, Yoram
AU  - Grossman Y
AD  - Department of Physiology and Cell Biology, Faculty of Health Sciences and 
      Zlotowski Center for Neuroscience, Ben Gurion University of the Negev, 
      Beer-Sheva, 84105, Israel.
LA  - eng
SI  - GENBANK/AF001423
SI  - GENBANK/U08261
SI  - GENBANK/U08262
SI  - GENBANK/U08263
SI  - GENBANK/U08264
SI  - GENBANK/U08265
SI  - GENBANK/U08266
SI  - GENBANK/U08267
SI  - GENBANK/U08268
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20150811
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Protein Isoforms)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
SB  - IM
MH  - Animals
MH  - High Pressure Neurological Syndrome/metabolism
MH  - Membrane Potentials/physiology
MH  - Molecular Sequence Data
MH  - Oocytes
MH  - *Pressure/adverse effects
MH  - Protein Isoforms
MH  - Receptors, N-Methyl-D-Aspartate/genetics/*metabolism
MH  - Xenopus laevis
OTO - NOTNLM
OT  - Xenopus laevis
OT  - central nervous system hyperexcitability
OT  - diving physiology
OT  - high-pressure neurological syndrome
OT  - oocyte
OT  - pressure
EDAT- 2015/07/24 06:00
MHDA- 2016/08/04 06:00
CRDT- 2015/07/24 06:00
PHST- 2015/01/15 00:00 [received]
PHST- 2015/07/12 00:00 [revised]
PHST- 2015/07/16 00:00 [accepted]
PHST- 2015/07/24 06:00 [entrez]
PHST- 2015/07/24 06:00 [pubmed]
PHST- 2016/08/04 06:00 [medline]
AID - 10.1111/ejn.13022 [doi]
PST - ppublish
SO  - Eur J Neurosci. 2015 Oct;42(8):2577-84. doi: 10.1111/ejn.13022. Epub 2015 Aug 11.