PMID- 26204790 OWN - NLM STAT- MEDLINE DCOM- 20160509 LR - 20221109 IS - 1399-0039 (Electronic) IS - 0001-2815 (Linking) VI - 86 IP - 3 DP - 2015 Sep TI - Bronchiolitis obliterans syndrome due to donor-specific HLA-antibodies. PG - 178-85 LID - 10.1111/tan.12626 [doi] AB - Chronic lung allograft dysfunction (CLAD) is a limiting factor for long-term survival in lung transplant recipients. Donor-specific human leukocyte antigen (HLA)-antibodies (DSA) have been suggested as potential risk factors for CLAD. However, their impact on clinical outcome following lung transplantation remains controversial. We performed a single-center study of 120 lung transplant recipients transplanted between 2006 and 2011. Patient sera were investigated before and after transplantation. The sera were screened by means of Luminex((R)) technology (Luminex Inc., Austin, TX, USA) for IgG-HLA-class I and class II antibodies (ab). Using single antigen beads, DSA were identified and correlated retrospectively with clinical parameters. After transplantation 39 out of 120 patients (32.5%) were positive for HLA-ab. The incidence of de novo DSA formation was 27 of 120 patients (22.5%). Eleven of 27 (41%) of de novo DSA-positive patients developed BOS compared to 13 of 93 (14%) DSA-negative patients (p = 0.002). Furthermore, the generation of de novo DSA was independently associated with the development of BOS in multivariable analysis [hazard ration (HR) 2.5, 95% confidence interval (CI) 1.0-6.08; p = 0.046). Our results indicate that de novo DSA are associated with the development of BOS after lung transplantation. Monitoring of HLA-ab after transplantation is useful for identifying high-risk patients and offers an opportunity for early therapeutic intervention. CI - (c) 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. FAU - Kauke, T AU - Kauke T AD - Laboratory for Immunogenetics, University Hospital Grosshadern, Ludwig-Maximilians-University, Munich, Germany. AD - Department of General, Visceral, Transplantation, Vascular and Thoracic Surgery, University Hospital Grosshadern, Ludwig-Maximilians-University, Munich, Germany. FAU - Kneidinger, N AU - Kneidinger N AD - Department of Internal Medicine V, University Hospital Grosshadern, Ludwig-Maximilians-University, and Asklepios Fachkliniken Munchen-Gauting, Comprehensive Pneumology Center Munich (CPC-M), Member of the German Center for Lung Research (DZL), Munich, Germany. FAU - Martin, B AU - Martin B AD - Department of Internal Medicine V, University Hospital Grosshadern, Ludwig-Maximilians-University, and Asklepios Fachkliniken Munchen-Gauting, Comprehensive Pneumology Center Munich (CPC-M), Member of the German Center for Lung Research (DZL), Munich, Germany. FAU - Dick, A AU - Dick A AD - Laboratory for Immunogenetics, University Hospital Grosshadern, Ludwig-Maximilians-University, Munich, Germany. FAU - Schneider, C AU - Schneider C AD - Department of General, Visceral, Transplantation, Vascular and Thoracic Surgery, University Hospital Grosshadern, Ludwig-Maximilians-University, Munich, Germany. FAU - Schramm, R AU - Schramm R AD - Department of Cardiac Surgery, University Hospital Grosshadern, Ludwig-Maximilians-University, Munich, Germany. FAU - Meimarakis, G AU - Meimarakis G AD - Department of General, Visceral, Transplantation, Vascular and Thoracic Surgery, University Hospital Grosshadern, Ludwig-Maximilians-University, Munich, Germany. FAU - Preissler, G AU - Preissler G AD - Department of General, Visceral, Transplantation, Vascular and Thoracic Surgery, University Hospital Grosshadern, Ludwig-Maximilians-University, Munich, Germany. FAU - Eickelberg, O AU - Eickelberg O AD - Comprehensive Pneumology Center Munich (CPC-M), Institute of Lung Biology and Disease, Helmholtz Zentrum Munchen, Member of the German Center for Lung Research (DZL), Munich, Germany. FAU - von Dossow, V AU - von Dossow V AD - Department of Anaesthesiology, University Hospital Grosshadern, Ludwig-Maximilians-University, Munich Lung Transplant Group (MLTP), Munich, Germany. FAU - Behr, J AU - Behr J AD - Department of Internal Medicine V, University Hospital Grosshadern, Ludwig-Maximilians-University, and Asklepios Fachkliniken Munchen-Gauting, Comprehensive Pneumology Center Munich (CPC-M), Member of the German Center for Lung Research (DZL), Munich, Germany. FAU - Hatz, R AU - Hatz R AD - Department of General, Visceral, Transplantation, Vascular and Thoracic Surgery, University Hospital Grosshadern, Ludwig-Maximilians-University, Munich, Germany. FAU - Neurohr, C AU - Neurohr C AD - Department of Internal Medicine V, University Hospital Grosshadern, Ludwig-Maximilians-University, and Asklepios Fachkliniken Munchen-Gauting, Comprehensive Pneumology Center Munich (CPC-M), Member of the German Center for Lung Research (DZL), Munich, Germany. FAU - Winter, H AU - Winter H AD - Department of General, Visceral, Transplantation, Vascular and Thoracic Surgery, University Hospital Grosshadern, Ludwig-Maximilians-University, Munich, Germany. LA - eng PT - Journal Article DEP - 20150723 PL - England TA - Tissue Antigens JT - Tissue antigens JID - 0331072 RN - 0 (Antibodies) RN - 0 (HLA Antigens) SB - IM MH - Adult MH - Antibodies/*immunology MH - Bronchiolitis Obliterans/*immunology MH - Female MH - HLA Antigens/*immunology MH - Humans MH - Lung Transplantation MH - Male MH - Middle Aged MH - Tissue Donors OTO - NOTNLM OT - bronchiolitis obliterans syndrome OT - chronic lung allograft dysfunction OT - human leukocyte antigen-antibodies OT - humoral rejection OT - lung transplantation EDAT- 2015/07/25 06:00 MHDA- 2016/05/10 06:00 CRDT- 2015/07/25 06:00 PHST- 2015/03/14 00:00 [received] PHST- 2015/06/13 00:00 [revised] PHST- 2015/06/27 00:00 [accepted] PHST- 2015/07/25 06:00 [entrez] PHST- 2015/07/25 06:00 [pubmed] PHST- 2016/05/10 06:00 [medline] AID - 10.1111/tan.12626 [doi] PST - ppublish SO - Tissue Antigens. 2015 Sep;86(3):178-85. doi: 10.1111/tan.12626. Epub 2015 Jul 23.