PMID- 26205216
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20181202
IS  - 1708-0428 (Electronic)
IS  - 0960-8923 (Linking)
VI  - 26
IP  - 5
DP  - 2016 May
TI  - Duodenal-Jejunal Bypass Surgery Ameliorates Glucose Homeostasis and Reduces 
      Endoplasmic Reticulum Stress in the Liver Tissue in a Diabetic Rat Model.
PG  - 1002-9
LID - 10.1007/s11695-015-1816-2 [doi]
AB  - BACKGROUND: Duodenal-jejunal bypass (DJB) has been shown to be an effective 
      surgical treatment for type 2 diabetes mellitus (T2DM). However, the underlying 
      mechanisms are poorly understood. Recently, accumulating evidences suggest that 
      endoplasmic reticulum (ER) stress plays an important role in the development of 
      insulin resistance in T2DM. The present study was designed to investigate the 
      effect of DJB on glucose homeostasis, the ER stress state in the liver tissue, 
      and the involving signaling independently of weight loss. METHODS: Thirty adult 
      male T2DM Sprague-Dawley (SD) rats induced by high-fat diet and low dose of 
      streptozotocin (STZ) were randomly divided into DJB and sham groups. Ten 
      age-matched male SD rats were assigned as the control group. The parameters of 
      body weight and calorie intake were measured at indicated time points. The 
      glucose tolerance and insulin resistance were detected to evaluate the glucose 
      homeostasis. Serum insulin was determined by enzyme-linked immunosorbent assay 
      (ELISA). The markers of ER stress, the activity of c-Jun N-terminal kinase (JNK) 
      and serine phosphorylation of insulin receptor substrate 1 (IRS-1) in the liver 
      tissue, were determined by Western blotting. RESULTS: DJB induced significant 
      improvements in glucose homeostasis and insulin sensitivity, but without weight 
      loss. DJB improved the ER stress state indicated by decreased protein kinase RNA 
      (PKR)-like ER protein kinase (PERK) and inositol-requiring enzyme 1 (IRE-1) 
      phosphorylation in the liver tissue. The JNK activity and serine phosphorylation 
      of IRS-1 in the liver tissue were significantly reduced after DJB. CONCLUSIONS: 
      DJB ameliorates glucose homeostasis. Meanwhile, our study helps to reveal that 
      the reduced hepatic ER stress and the decreased JNK activity may contribute to 
      the improved glucose homeostasis after DJB.
FAU - Li, Maogang
AU  - Li M
AD  - Department of General Surgery, Qilu Hospital of Shandong University, No. 107, 
      Wenhuaxi Road, Jinan, 250012, Shandong, People's Republic of China.
AD  - Department of General Surgery, Laigang Hospital Affiliated to Taishan Medical 
      University, No. 68, Xinxing Road, Laiwu, 271126, Shandong, People's Republic of 
      China.
FAU - Li, Hengping
AU  - Li H
AD  - Department of General Surgery, Laigang Hospital Affiliated to Taishan Medical 
      University, No. 68, Xinxing Road, Laiwu, 271126, Shandong, People's Republic of 
      China.
FAU - Zhou, Zhigang
AU  - Zhou Z
AD  - Department of General Surgery, Laigang Hospital Affiliated to Taishan Medical 
      University, No. 68, Xinxing Road, Laiwu, 271126, Shandong, People's Republic of 
      China.
FAU - Zhou, Yanggui
AU  - Zhou Y
AD  - Department of Internal Medicine, Laigang Hospital Affiliated to Taishan Medical 
      University, No. 68, Xinxing Road, Laiwu, 271126, Shandong, People's Republic of 
      China.
FAU - Wang, Yanmin
AU  - Wang Y
AD  - Department of General Surgery, Qilu Hospital of Shandong University, No. 107, 
      Wenhuaxi Road, Jinan, 250012, Shandong, People's Republic of China.
FAU - Zhang, Xiang
AU  - Zhang X
AD  - Department of General Surgery, Qilu Hospital of Shandong University, No. 107, 
      Wenhuaxi Road, Jinan, 250012, Shandong, People's Republic of China.
FAU - Liu, Teng
AU  - Liu T
AD  - Department of General Surgery, Qilu Hospital of Shandong University, No. 107, 
      Wenhuaxi Road, Jinan, 250012, Shandong, People's Republic of China.
FAU - Zhong, Mingwei
AU  - Zhong M
AD  - Department of General Surgery, Qilu Hospital of Shandong University, No. 107, 
      Wenhuaxi Road, Jinan, 250012, Shandong, People's Republic of China.
FAU - Han, Haifeng
AU  - Han H
AD  - Department of General Surgery, Qilu Hospital of Shandong University, No. 107, 
      Wenhuaxi Road, Jinan, 250012, Shandong, People's Republic of China.
FAU - Liu, Shaozhuang
AU  - Liu S
AD  - Department of General Surgery, Qilu Hospital of Shandong University, No. 107, 
      Wenhuaxi Road, Jinan, 250012, Shandong, People's Republic of China.
FAU - Hu, Sanyuan
AU  - Hu S
AD  - Department of General Surgery, Qilu Hospital of Shandong University, No. 107, 
      Wenhuaxi Road, Jinan, 250012, Shandong, People's Republic of China. 
      husanyuan1962@hotmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Obes Surg
JT  - Obesity surgery
JID - 9106714
RN  - 5W494URQ81 (Streptozocin)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Diabetes Mellitus, Experimental/metabolism/pathology/*surgery
MH  - Diabetes Mellitus, Type 2/metabolism/pathology/*surgery
MH  - Duodenum/surgery
MH  - *Endoplasmic Reticulum Stress
MH  - Glucose/*metabolism
MH  - Homeostasis
MH  - Insulin Resistance
MH  - *Jejunoileal Bypass/methods
MH  - Jejunum/surgery
MH  - Liver/*metabolism/pathology
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Streptozocin
OTO - NOTNLM
OT  - Duodenal-jejunal bypass
OT  - Endoplasmic reticulum stress
OT  - Glucose homeostasis
OT  - Hepatic insulin resistance
OT  - Liver tissue
OT  - Type 2 diabetes mellitus
OT  - c-Jun N-terminal kinase
EDAT- 2015/07/25 06:00
MHDA- 2016/12/15 06:00
CRDT- 2015/07/25 06:00
PHST- 2015/07/25 06:00 [entrez]
PHST- 2015/07/25 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - 10.1007/s11695-015-1816-2 [pii]
AID - 10.1007/s11695-015-1816-2 [doi]
PST - ppublish
SO  - Obes Surg. 2016 May;26(5):1002-9. doi: 10.1007/s11695-015-1816-2.