PMID- 26207150
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150724
LR  - 20181113
IS  - 1868-7075 (Print)
IS  - 1868-7083 (Electronic)
IS  - 1868-7075 (Linking)
VI  - 7
IP  - 1
DP  - 2015
TI  - DNA hydroxymethylation age of human blood determined by capillary 
      hydrophilic-interaction liquid chromatography/mass spectrometry.
PG  - 72
LID - 10.1186/s13148-015-0109-x [doi]
LID - 72
AB  - BACKGROUND: Aging is a complex phenomenon and characterized by a progressive 
      decline in physiology and function of adult tissues. However, it hasn't been well 
      established of the correlation between aging and global DNA methylation and 
      hydroxymethylation that regulate the growth and development of higher organisms. 
      RESULTS: We developed an on-line trapping/capillary hydrophilic-interaction 
      liquid chromatography/electrospray ionization-mass spectrometry method for 
      ultra-sensitive and simultaneous quantification of 5-methylcytosine (5-mC) and 
      5-hydroxymethylcytosine (5-hmC) in genomic DNA from human blood. Limits of 
      detection for 5-mC and 5-hmC were 0.04 and 0.13 fmol, respectively. The 
      imprecision and recovery of the method were determined with the relative standard 
      deviations (RSDs) and relative errors being <11.2 and 14.0 %, respectively. We 
      analyzed the contents of 5-mC and 5-hmC in genomic DNA of blood from 238 healthy 
      people aged from 1 to 82 years. The results showed that 5-hmC content was 
      significantly decreased and highly correlated with aging process, while 5-mC only 
      showed slight correlation with age. We then established a DNA hydroxymethylation 
      age model according to 5-hmC content with a mean absolute deviation (MAD) of 
      approximate 8.9 years. We also calculated the mean relative error (MRE) using the 
      predicted ages based on the age model and the chronological ages. The results 
      showed that the MRE was 18.3 % for samples with ages from 20 to 82 years (95 % 
      confidence interval, N = 190). CONCLUSIONS: The global DNA hydroxymethylation 
      represents a strong and reproducible mark of chronological age, which could be 
      potentially applied in health assessment and prevention of diseases. The 
      identification of biological or environmental factors that influence DNA 
      hydroxymethylation aging rate may permit quantitative assessments of their 
      impacts on health.
FAU - Xiong, Jun
AU  - Xiong J
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      education), Department of Chemistry, Wuhan University, Wuhan, 430072 China.
FAU - Jiang, Han-Peng
AU  - Jiang HP
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      education), Department of Chemistry, Wuhan University, Wuhan, 430072 China.
FAU - Peng, Chun-Yan
AU  - Peng CY
AD  - Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan, 430071 
      China.
FAU - Deng, Qian-Yun
AU  - Deng QY
AD  - Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan, 430071 
      China.
FAU - Lan, Meng-Dan
AU  - Lan MD
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      education), Department of Chemistry, Wuhan University, Wuhan, 430072 China.
FAU - Zeng, Huan
AU  - Zeng H
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      education), Department of Chemistry, Wuhan University, Wuhan, 430072 China.
FAU - Zheng, Fang
AU  - Zheng F
AD  - Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan, 430071 
      China.
FAU - Feng, Yu-Qi
AU  - Feng YQ
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      education), Department of Chemistry, Wuhan University, Wuhan, 430072 China.
FAU - Yuan, Bi-Feng
AU  - Yuan BF
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      education), Department of Chemistry, Wuhan University, Wuhan, 430072 China.
LA  - eng
PT  - Journal Article
DEP - 20150723
PL  - Germany
TA  - Clin Epigenetics
JT  - Clinical epigenetics
JID - 101516977
PMC - PMC4511989
OTO - NOTNLM
OT  - 5-hydroxymethylcytosine
OT  - 5-methylcytosine
OT  - Aging
OT  - Hydrophilic-interaction liquid chromatography
OT  - Mass spectrometry
EDAT- 2015/07/25 06:00
MHDA- 2015/07/25 06:01
PMCR- 2015/07/23
CRDT- 2015/07/25 06:00
PHST- 2015/04/08 00:00 [received]
PHST- 2015/07/03 00:00 [accepted]
PHST- 2015/07/25 06:00 [entrez]
PHST- 2015/07/25 06:00 [pubmed]
PHST- 2015/07/25 06:01 [medline]
PHST- 2015/07/23 00:00 [pmc-release]
AID - 109 [pii]
AID - 10.1186/s13148-015-0109-x [doi]
PST - epublish
SO  - Clin Epigenetics. 2015 Jul 23;7(1):72. doi: 10.1186/s13148-015-0109-x. 
      eCollection 2015.