PMID- 26207867
OWN - NLM
STAT- MEDLINE
DCOM- 20160506
LR  - 20150803
IS  - 1532-4311 (Electronic)
IS  - 0882-0139 (Linking)
VI  - 44
IP  - 6
DP  - 2015
TI  - TNF-alpha Production by Peripheral Blood Monocytes in Multiple Sclerosis Patients 
      and Healthy Controls.
PG  - 590-601
LID - 10.3109/08820139.2015.1059851 [doi]
AB  - BACKGROUND: Aberrant immune responses are evident in the pathogenesis of multiple 
      sclerosis (MS) and it has been proposed that the spectrum of cytokines influence 
      disease outcomes. Leptin and lipopolysaccharide (LPS) of Gram-negative bacteria 
      are both potent cellular stimulators for production of pro-inflammatory cytokines 
      such as tumor necrosis factor-alpha (TNF-alpha). The aim of this study was to compare the 
      TNF-alpha production by peripheral blood monocytes from MS patients with healthy 
      controls. METHODS: Peripheral blood samples were stimulated with LPS or leptin. 
      After blocking the Golgi apparatus, intracellular cytokine production was 
      assessed using a monoclonal antibody against human TNF-alpha by the flow cytometry 
      technique. Moreover, plasma level measurement of cytokines was performed using 
      enzyme-linked immunosorbent assay (ELISA). RESULTS: Intracellular levels of TNF-alpha 
      were 16.80 +/- 8.21 and 16.52 +/- 8.23in MS patients and healthy controls which 
      showed no statistically significant difference between them (p = 0.850). 
      Leptin-stimulated and LPS-stimulated TNF-alpha production showed no significant 
      difference between MS patients and the control group (p = 0.263 and p = 0.191, 
      respectively). However, after treatment with leptin, a weak significant 
      difference was shown between cases and control group (p = 0.049). There were 
      significant differences between cases and controls regarding serum levels of IL-6 
      and Toll-like receptor-4 (TLR-4) before and after stimulation with leptin and 
      LPS, separately (p < 0.05). CONCLUSION: Taken together, we cannot definitely 
      conclude that TNF-alpha does not play an important role in pathogenesis of MS. 
      However, other characteristics of monocyte activation such as IL-6 or TLRs can 
      elucidate implication of peripheral blood monocytes in MS pathogenesis.
FAU - Farrokhi, Mehrdad
AU  - Farrokhi M
AD  - Department of Neurology, School of Medicine, Isfahan University of Medical 
      Sciences , Isfahan , Iran .
FAU - Etemadifar, Masoud
AU  - Etemadifar M
FAU - Jafary Alavi, Maryam Sadat
AU  - Jafary Alavi MS
FAU - Zarkesh-Esfahani, Sayyed Hamid
AU  - Zarkesh-Esfahani SH
FAU - Behjati, Mohaddeseh
AU  - Behjati M
FAU - Rezaei, Ali
AU  - Rezaei A
FAU - Amani-Beni, Ali
AU  - Amani-Beni A
LA  - eng
PT  - Journal Article
DEP - 20150724
PL  - England
TA  - Immunol Invest
JT  - Immunological investigations
JID - 8504629
RN  - 0 (IL6 protein, human)
RN  - 0 (Interleukin-6)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adult
MH  - Female
MH  - Humans
MH  - Interleukin-6/blood
MH  - Male
MH  - Monocytes/*immunology
MH  - Multiple Sclerosis/*immunology
MH  - Toll-Like Receptor 4/immunology
MH  - Tumor Necrosis Factor-alpha/blood/*immunology
MH  - Young Adult
OTO - NOTNLM
OT  - Flow cytometry
OT  - leptin
OT  - multiple sclerosis
OT  - tumor necrosis factor-alpha
EDAT- 2015/07/25 06:00
MHDA- 2016/05/07 06:00
CRDT- 2015/07/25 06:00
PHST- 2015/07/25 06:00 [entrez]
PHST- 2015/07/25 06:00 [pubmed]
PHST- 2016/05/07 06:00 [medline]
AID - 10.3109/08820139.2015.1059851 [doi]
PST - ppublish
SO  - Immunol Invest. 2015;44(6):590-601. doi: 10.3109/08820139.2015.1059851. Epub 2015 
      Jul 24.